. "The project results were published in two original articles and one review in impacted journals and two posters in international conferences. The goals declared in the projects were fulfilled; modifications of the work lead to additional results with respect to the original goals. A collaborator will use some of the results for a joint project with a leading Swiss proteomic laboratory."@en . "Anal\u00FDza fosfoproteomu leukemick\u00FDch bun\u011B\u010Dn\u00FDch lini\u00ED po oz\u00E1\u0159en\u00ED." . "V\u00FDsledky projektu byly publikov\u00E1ny ve dvou origin\u00E1ln\u00EDch prac\u00EDch a jedn\u00E9 re\u0161er\u0161i v impaktovan\u00FDch \u010Dasopisech a jako dva postery na zahrani\u010Dn\u00EDch konferenc\u00EDch. C\u00EDle deklarovan\u00E9 v projektu byly spln\u011Bny; \u00FApravy \u0159e\u0161en\u00ED vedly k z\u00EDsk\u00E1n\u00ED v\u00FDsledk\u016F nad r\u00E1mec p\u016Fvodn\u00EDch c\u00EDl\u016F. Na n\u011Bkter\u00E9 z v\u00FDsledk\u016F nav\u00E1\u017Ee spolupracovnice v r\u00E1mci spole\u010Dn\u00E9ho projektu s p\u0159edn\u00ED \u0161v\u00FDcarskou proteomickou laborato\u0159\u00ED."@cs . . . . . "Phosphorylation is one of the most important post-translational modifications, which affects protein structure, function, and localization, regulating majority of biochemical processes. In our previous work we were engaged with molecular mechanisms of response of leukaemic cells MOLT-4 (p53-positive) and HL-60 (p53-negative)to ionising radiation. Submitted project proposes to characterize phosphoproteome of the mentioned cells, i.e. to identify phosphoproteins which are involved in radiation-induced signaling using tandem mass spectrometry, and to propose the potential biodosimetric markers. To date, no study has been published in the presented area mainly due to a very low abundance of phosphoproteins in the cell and due to insufficient ionization during MS analysis (acidic and hydrophilic character) compared to non-modified proteins. Therefore we will employ the newest methods for selective enrichment of phosphopeptides (bioaffinity chromatography) and recent knowledge to increase enrichment efficiency (methylation, pH adjustment etc.)."@en . . "http://www.isvav.cz/projectDetail.do?rowId=GPP206/12/P338"^^ . "Fosforylace je jednou z nejd\u016Fle\u017Eit\u011Bj\u0161\u00EDch posttransla\u010Dn\u00EDch modifikac\u00ED, kter\u00E1 ovliv\u0148uje funkci, strukturu a lokalizaci proteinu a pomoc\u00ED n\u00ED\u017E je regulov\u00E1na v\u011Bt\u0161ina biochemick\u00FDch proces\u016F. V na\u0161\u00ED p\u0159edchoz\u00ED pr\u00E1ci jsme se zab\u00FDvali molekul\u00E1rn\u00EDmi mechanismy odpov\u011Bdi leukemick\u00FDch bun\u011Bk MOLT-4 (p53-positivn\u00ED) a HL-60 (p53-negativn\u00ED) na oz\u00E1\u0159en\u00ED. P\u0159edkl\u00E1dan\u00FD projekt si klade za c\u00EDl charakterizovat fosfoproteom t\u011Bchto bun\u011Bk, tj. identifikovat fosfoproteiny, kter\u00E9 jsou sou\u010D\u00E1st\u00ED sign\u00E1ln\u00EDch drah spou\u0161t\u011Bn\u00FDch po expozici ionizuj\u00EDc\u00EDmu z\u00E1\u0159en\u00ED, d\u00E1le ur\u010Dit p\u0159esn\u00E1 m\u00EDsta fosforylace pomoc\u00ED tandemov\u00E9 hmotnostn\u00ED spektrometrie a navrhnout potenci\u00E1ln\u00ED biodosimetrick\u00E9 markery. Doposud v tomto sm\u011Bru nebyla publikov\u00E1na \u017E\u00E1dn\u00E1 pr\u00E1ce a to zejm\u00E9na proto, \u017Ee absolutn\u00ED mno\u017Estv\u00ED fosfoprotein\u016F v bu\u0148ce je velmi mal\u00E9 a vzhledem ke kysel\u00E9mu a hydrofiln\u00EDmu charakteru je \u00FA\u010Dinnost jejich ionizace p\u0159i MS anal\u00FDz\u00E1ch podstatn\u011B ni\u017E\u0161\u00ED ne\u017E u nemodifikovan\u00FDch peptid\u016F. Proto hodl\u00E1me zapojit nejnov\u011Bj\u0161\u00ED metody pro selektivn\u00ED obohacen\u00ED fosfopeptid\u016F (bioafinitn\u00ED chromatografie) a vyu\u017E\u00EDt nov\u00FDch poznatk\u016F vedouc\u00EDch k optimalizaci dan\u00E9 metodiky." . "2014-12-31+01:00"^^ . "Phosphoproteomic analysis of leukaemic cells after irradiation."@en . . . "0"^^ . . . . . . . "GPP206/12/P338" . "1"^^ . . "2015-05-22+02:00"^^ . "2012-01-01+01:00"^^ . . . "2014-02-28+01:00"^^ . "3"^^ . "0"^^ . "phosphoproteins mass spectrometry leukaemia ionising radiation"@en . "3"^^ . .