. . "2010-04-16+02:00"^^ . "0"^^ . "AtTRB proteins are members of the SMH family defined by N-terminal Myb domain, central region with H1/5 domain and coiled-coil domain near C-terminus. SMH family is exclusively plant specific. Members of the SMH family are not only telomeric sequence binding due to their telobox-type Myb domain but they are also likely to regulate promotor sequences in various genes. So far we have characterized three SMH proteins. Although each of them is ds-telomeric repeat binding, some of them are also G-rich strand binding and they further differ in their ability to interact with other proteins, as was determined by the yeast two-hybrid system. The aim of this project is to find interaction partners of the SMH proteins directly in plant system via TAP-tag which allows two-step protein purification under native conditions. This project should also answer the question whether the AtTRB proteins are telomere localized or whether they distinguish interstitial telomeric repeats within promoter regions. The"@en . "2015-03-20+01:00"^^ . . . "protein expression; protein-protein interaction; DNA-protein binding; chromatin immunoprecipitation"@en . "1"^^ . "Proteiny AtTRB pat\u0159\u00ED do skupiny SMH protein\u016F, kter\u00E9 jsou definov\u00E1ny p\u0159\u00EDtomnost\u00ED Myb dom\u00E9ny na sv\u00E9m N-konci, centr\u00E1ln\u00ED H1/5 dom\u00E9nou a C-koncovou coiled-coil dom\u00E9nou. Proteiny typu SMH jsou specifick\u00E9 pouze pro rostlinnou \u0159\u00ED\u0161i a jejich \u010Dlenov\u00E9 se v\u00E1\u017E\u00ED Myb dom\u00E9nou typu telobox nejen na rostlinn\u00E9 telomerick\u00E9 repetice, ale tak\u00E9 mohou m\u00EDt regula\u010Dn\u00ED funkci v promotorech jin\u00FDch gen\u016F. Do sou\u010Dasn\u00E9 doby byly v na\u0161\u00ED laborato\u0159i charakterizov\u00E1ny t\u0159i proteiny z t\u00E9to skupiny. A\u010Dkoliv byla u v\u0161ech protein\u016F prok\u00E1z\u00E1na specifick\u00E1 vazba na ds-telomerick\u00E9 repetice a u n\u011Bkter\u00FDch tak\u00E9 na G-bohat\u00E9 vl\u00E1kno telomerick\u00E9 repetice, v dvojhybridn\u00EDm syst\u00E9mu se jednotliv\u00E9 proteiny v\u00FDznamn\u011B li\u0161\u00ED sv\u00FDmi interak\u010Dn\u00EDmi partnery. C\u00EDlem tohoto projektu je vyhledat interak\u010Dn\u00ED partnery p\u0159\u00EDmo v rostlinn\u00E9m syst\u00E9mu pomoc\u00ED metody TAP-tag, kter\u00E1 umo\u017E\u0148uje \u0161etrnou dvojkrokovou purifikaci protein\u016F. Projekt by m\u011Bl tak\u00E9 s pou\u017Eit\u00EDm ChIP anal\u00FDzy pomoci vy\u0159e\u0161it ot\u00E1zku, zda jsou AtTRB proteiny lokalizov\u00E1ny na telomer\u00E1ch a nebo rozezn\u00E1vaj\u00ED interstici\u00E1ln\u00ED" . "protein expression" . "0"^^ . "Characterisation of functional diversity of the plant-specific SMH family of proteins"@en . . . "17"^^ . "The work on the project \u201CCharacterisation of functional diversity of the plant-specific SMH family of proteins\u201D has brought original results regarding the analysis of the role of the plant AtTRB proteins in vitro or in vivo. The solution of the project has resulted in the following outputs:\u00A0 clarification of the AtTRB binding specificity; design of a binding model of the separate AtTRB domains to"@en . "17"^^ . . . . "Grantov\u00E1 agentura \u010Cesk\u00E9 republiky Grantov\u00E1 agentura \u010Cesk\u00E9 republiky \u0158e\u0161en\u00ED projektu \\%22Charakterizace a funk\u010Dn\u00ED diverzita protein\u016F rodiny SMH, kter\u00E1 je specifick\u00E1 pro rostliny\\%22 p\u0159ineslo p\u016Fvodn\u00ED v\u00FDsledky v oblasti anal\u00FDzy chov\u00E1n\u00ED rostlinn\u00FDch AtTRB protein\u016F in vitro i in vivo. V r\u00E1mci projektu byla zp\u0159esn\u011Bna jejich vazebn\u00E1 specificita, navr\u017Een model vazby jednotliv\u00FDch dom\u00E9n AtTRB protein"@cs . . " protein-protein interaction" . . . "GP521/08/P452" . "2010-12-31+01:00"^^ . . . . . "2008-01-01+01:00"^^ . . . "http://www.isvav.cz/projectDetail.do?rowId=GP521/08/P452"^^ . "Charakterizace a funk\u010Dn\u00ED diverzita protein\u016F rodiny SMH, kter\u00E1 je specifick\u00E1 pro rostliny" . " DNA-protein binding" . .