"6"^^ . . "Dendritic cells (DC) represent potent antigen presenting cells crucial in generation and regulation of immunity as well as tolerance, therefore it is of a particular interest to search for substances with adjuvant properties to instruct DC to modulate T cell-mediated immune responses. The adjuvant nature of cAMP signalling generated by cholera toxin is well documented. Here, we propose to investigate adjuvant activity of B. pertussis adenylate cyclase toxin (CyaA) to induce DC maturation and modulate the ability of DC to prime antigen specific CD4+ and CD8+ T cells in respect to their potential use in immunotherapy. Furthermore, the generation of antigen-induced T regulatory cells by CyaA-treated DC shall be examined together with their potential to prevent the onset of experimental autoimmune encephalomyelitis in mouse model. Also the possibility of expansion of CD4+CD25+Fox3p+ Treg by CyaA-treated DC will be tested. Ex vivo CyaA-treated DC might present an elegant strategy how to use adjuvant properties of CyaA-mediated cAMP signalling and bypass its possible toxic effects in vivo.\u00A0"@en . . "0"^^ . "Adjuvant and immunotherapeutic properties of Bordetella pertussis adenylate cyclase toxin"@en . "1"^^ . . "\u0158e\u0161en\u00ED projektu prob\u011Bhlo na vynikaj\u00EDc\u00ED \u00FArovni, a to jak z hlediska odborn\u00E9ho tak i z hlediska \u010Derp\u00E1n\u00ED finan\u010Dn\u00EDch prost\u0159edk\u016F."@cs . " cAMP" . . "0"^^ . . . . . . "dendritick\u00E9 bu\u0148ky; CyaA; cAMP; adjuvans,T cells"@en . . "dendritick\u00E9 bu\u0148ky" . "2009-01-01+01:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=GP310/09/P582"^^ . " adjuvans" . . "C\u00EDlem projektu je charakterizovat vliv p\u016Fsoben\u00ED adenyl\u00E1t-cykl\u00E1zov\u00E9ho toxinu na maturaci dendritick\u00FDch bun\u011Bk a na jejich schopnost indukova specifick\u00E9 T bun\u011B\u010Dn\u00E9 odpov\u011Bdi, v\u010Detn\u011B expanze T regula\u010Dn\u00EDch bun\u011Bk s imunoterapeutick\u00FDm potenci\u00E1lem." . "The project was excellent, both in terms of expertise and in terms of disbursement of funds."@en . "2011-04-16+02:00"^^ . . . "2011-12-31+01:00"^^ . . . . "2015-03-02+01:00"^^ . "GP310/09/P582" . "Adjuvantn\u00ED a imunoterapeutick\u00E9 vlastnosti adenyl\u00E1t-cykl\u00E1zov\u00E9ho toxinu z Bordetella pertussis" . . "6"^^ . " CyaA" .