"2008-01-01+01:00"^^ . . "C\u00EDlem projektu bylo: syntetizovat a charakterizovat dithiokarbam\u00E1tov\u00E9 komplexy platnat\u00E9 a zlatit\u00E9. Ur\u010Dit toxicitu t\u011Bchto l\u00E1tek pro jatern\u00ED n\u00E1dorov\u00E9 linie HepG2 a SK-HEP-1 a d\u00E1le zjistit, zda inhibuj\u00ED jadern\u00FD faktor-kappaB (NF-\u03BAB) a proteazom v obou zm\u00EDn\u011Bn\u00FDch bun\u011B\u010Dn\u00FDch modelech. V\u011Bt\u0161inu t\u011Bchto c\u00EDl\u016F se poda\u0159ilo splnit, a to s neo\u010Dek\u00E1van\u00FDmi zji\u0161t\u011Bn\u00EDmi. Komplexy byly syntetizov\u00E1ny celkem 4, toti"@cs . . "2010-12-31+01:00"^^ . . " metal dithiocarbamates" . "2010-04-16+02:00"^^ . "Vliv dithiokarbam\u00E1tov\u00FDch komplex\u016F platnat\u00FDch a zlatit\u00FDch na NF-?B dr\u00E1hu v lidsk\u00FDch n\u00E1dorov\u00FDch lini\u00EDch HepG2 a SK-HEP-1" . . "Vliv dithiokarbam\u00E1tov\u00FDch komplex\u016F platnat\u00FDch a zlatit\u00FDch na NF-?B dr\u00E1hu v lidsk\u00FDch n\u00E1dorov\u00FDch lini\u00EDch HepG2 a SK-HEP-1\u00A0Jadern\u00FD faktor-?B (NF-?B) je st\u011B\u017Eejn\u00ED prvek v syst\u00E9mu regulace mnoha proz\u00E1n\u011Btliv\u00FDch gen\u016F, d\u00EDky \u010Demu\u017E je zaj\u00EDmav\u00FDm c\u00EDlem pro l\u00E9\u010Debn\u00FD z\u00E1sah. Jeho aktivace m\u016F\u017Ee tak\u00E9 \u0159\u00EDdit bujen\u00ED n\u00E1doru, zvy\u0161ovat schopnost jeho p\u0159e\u017Eit\u00ED a podporovat angiogenezi \u010Di vznik metast\u00E1z\u00ED. Dithiokarbam\u00E1ty a jejich komplexy s kovy jsou dlouho zn\u00E1my jako \u00FA\u010Dinn\u00E9 inhibitory NF-?B dr\u00E1hy, a\u010Dkoli dodnes neeexistuje na tomto poli systematick\u00FD v\u00FDzkum. P\u0159edkl\u00E1dan\u00FD projekt se zam\u011B\u0159uje na synt\u00E9zu a charakterizaci\u00A0platnat\u00FDch a zlatit\u00FDch\u00A0dithiokarbam\u00E1tov\u00FDch koordina\u010Dn\u00EDch slou\u010Denin, o nich\u017E se p\u0159edpokl\u00E1d\u00E1, \u017Ee potla\u010Duj\u00ED rakovinn\u00E9 bujen\u00ED, a zejm\u00E9na na studium jejich aktivity v\u016F\u010Di NF-?B a proteasomu.\u00A0L\u00E1tky budou testov\u00E1ny na dvou lidsk\u00FDch n\u00E1dorov\u00FDch lini\u00EDch (HepG2 a SK-HEP-1) s c\u00EDlem naj\u00EDt vhodn\u00E9 kandid\u00E1ty pro dal\u0161\u00ED preklinick\u00E9 studie." . . "Influence of dithiocarbamate complexes with Pt(II) and Au(III) on NF-?B pathway in human tumor lines HepG2 and SK-HEP-1"@en . . "http://www.isvav.cz/projectDetail.do?rowId=GP303/08/P137"^^ . . "Influence of dithiocarbamate complexes with Pt(II) and Au(III) on NF-?B pathway in human tumor lines HepG2 and SK-HEP-1\u00A0\u00A0\u00A0\u00A0\u00A0\u00A0\u00A0\u00A0\u00A0\u00A0\u00A0 Nuclear factor-?B (NF-?B) plays a pivotal in regulating expression of a number of proinflammatory genes makes it an attractive target for therapeutic intervention. Activation of NF-?B can also contribute to the oncogenic state in several ways: by driving proliferation, by ehancing cell survival, or by promoting angiogenesis or metastasis. Dithiocarbamates and their complexes with metals are long known and potent inhibitors of NF-?B pathway. However, there is no systematic research in this area up to this day. The present project focuses on synthesis and characterization of\u00A0Pt(II) and Au(III)\u00A0dithiocarbamate coordination compounds proposed to suppress cancer development and especially on study of their activity towards NF-?B and proteasome. Compounds will be tested in two human tumor lines (HepG2 and SK-HEP-1) to find suitable candidates for further"@en . "10"^^ . . . "10"^^ . . "1"^^ . "GP303/08/P137" . "NF-?B" . "2015-03-20+01:00"^^ . "0"^^ . "0"^^ . . . "NF-?B; metal dithiocarbamates; proteasome; inhibitors of proteasome; antitumor therapy"@en . . " inhibitors of proteasome" . . " proteasome" . . . "The aims of the project were: 1. to synthesize and characterize dithiocarbamate complexes with Pt(II) and Au(III); 2. to assess their toxicity in HepG2 and SK-HEP-1 cell lines; 3. to show if they are able to inhibit nuclear factor-kappaB (NF-\u03BAB) and proteasome in both cell lines. Most of the aims were achieved with unsuspected results. We prepared 4 complexes, 2 of them with diethy"@en . . . . .