. . "Significant changes in cellular morphology and physiology are connected with differentiation of hematopoietic cells. Namely, changes in amount, structure and localization of cytoskeletal protein vimentin have been described during this process. Fully developed filamentous network of vimentin was found in mature monocytes/macrophages. Besides its intracellular structural role, vimentin is also secreted to extracellular space where it participates in activation of phagocytic function of macrophages. The aim of this project is to explore the role of vimentin in the terminal differentiation of monocyte/macrophage cell lineage using v-myb transformed monoblastic cell line (BM2) as a model. We intend to address following specific aims: (1) to perform exact characterization of amount, structure and localization of vimentin during differentiation of BM2 cells induced by phorbol ester, trichostatin A and retinoic acid, (2) to perform analysis whether changes in expression of vimentin are essential for"@en . "The aim of this project was to investigate the role of vimentin in monocyte/macrophage differentiation pathway using the line of v-myb transformed monoblasts (BM2) as a model. We found that cellular level of vimentin increases in maturing BM2 cells treat"@en . . "10"^^ . "GP301/03/D022" . . . "http://www.isvav.cz/projectDetail.do?rowId=GP301/03/D022"^^ . "10"^^ . . "2007-10-16+02:00"^^ . "1"^^ . "The role of vimentin in the monocyte/macrophage differentiation pathway"@en . . "0"^^ . . . . . . "0"^^ . "Neuvedeno."@en . . . "P\u0159edlo\u017Een\u00FD projekt byl zam\u011B\u0159en na studium \u00FAlohy vimentinu p\u0159i termin\u00E1ln\u00ED diferenciaci bun\u011Bk monocytyck\u00E9/makrof\u00E1gov\u00E9 diferencia\u010Dn\u00ED dr\u00E1hy. K tomuto \u00FA\u010Delu byla jako model pou\u017Eita monoblastov\u00E1 bun\u011B\u010Dn\u00E1 linie transformovan\u00E1 onkogenem v-myb (BM2). Zjistili jsme"@cs . . . "\u00DAloha vimentinu v monocytick\u00E9/makrof\u00E1gov\u00E9 diferencia\u010Dn\u00ED dr\u00E1ze" . . . . "Diferenciace krevn\u00EDch bun\u011Bk je prov\u00E1zena v\u00FDznamn\u00FDmi zm\u011Bnami jejich morfologick\u00FDch a fyziologick\u00FDch vlastnost\u00ED. V jej\u00EDm pr\u016Fb\u011Bhu doch\u00E1z\u00ED nap\u0159. ke zm\u011Bn\u00E1m v mno\u017Estv\u00ED, struktu\u0159e a lokalizaci vimentinov\u00FDch filament, kter\u00E9 tvo\u0159\u00ED d\u016Fle\u017Eitou sou\u010D\u00E1st cytoskeletu. P\u0159edev\u0161\u00EDm zral\u00E9 bu\u0148ky monocyt\u00E1rn\u00ED/makrof\u00E1gov\u00E9 rady jsou charakterizov\u00E1ny pln\u011B rozvinutou s\u00EDt\u00ED vimentinov\u00FDch filament. Krom\u011B t\u00E9to strukturn\u00ED \u00FAlohy uvnit\u0159 bun\u011Bf je vimentin tak\u00E9 sekretov\u00E1n do mimobun\u011B\u010Dn\u00E9ho prostoru, kde se pod\u00EDl\u00ED na aktivaci fagocyt\u00E1rn\u00ED schopnosti makrof\u00E1g\u016F. P\u0159edlo\u017Een\u00FD projekt je zam\u011B\u0159en na studium \u00FAlohy vimentinu p\u0159i termin\u00E1ln\u00ED diferenciaci bun\u011Bk monocytyck\u00E9/makrof\u00E1gov\u00E9 diferencia\u010Dn\u00ED dr\u00E1hy. K tomuto \u00FA\u010Delu pou\u017Eijeme jako model monoblastovou bun\u011B\u010Dnou linii transformovanou onkogenem v-myb(BM2). C\u00EDlem projektu je (1) detailn\u00ED anal\u00FDza mno\u017Estv\u00ED, struktury a lokalizace vimentinu v pr\u016Fb\u011Bhu diferenciace t\u011Bchto bun\u011Bk indukovan\u00E9 forbolov\u00FDm esterem, trichostatinem A a kyselinou retinovou, (2) proveden\u00ED anal\u00FDzy, zda je zm\u011Bna hladiny vimentinu" . .