"2015-03-02+01:00"^^ . "0"^^ . . . . "1"^^ . . . . "3"^^ . "0"^^ . "3"^^ . " konjugace hormon\u016F" . . "\u0158e\u0161en\u00ED projektu prob\u011Bhlo podle pl\u00E1nu jak z hlediska odborn\u00E9ho tak i z hlediska \u010Derp\u00E1n\u00ED finan\u010Dn\u00EDch prost\u0159edk\u016F."@cs . " ektopick\u00E1 exprese" . . . . . "\u00DAloha vnitrobun\u011B\u010Dn\u00E9 kompartmentace reverzibiln\u00ED O-glukosylace v regulaci hladin aktivn\u00EDch forem cytokinin\u016F" . "GP204/09/P289" . "The project gone according to plan both in terms of expertise and in terms of disbursement of funds."@en . . . "2011-12-31+01:00"^^ . . . . " metabolismus cytokinin" . "2009-01-01+01:00"^^ . . . "2011-04-16+02:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GP204/09/P289"^^ . . "Charakterizace subcellul\u00E1rn\u00EDch variant Zm-p60.1 a jejich odpov\u011Bdi na cytokininy. Testov\u00E1n\u00ED odpov\u011Bdi v\u0161ech variant na ostatn\u00ED fytohormony, komponenty \u017Eivn\u00E9ho m\u00E9dia a fotoperiodu. P\u0159\u00EDprava a charakterizace transgenn\u00ED Arabidopsis nadprodukuj\u00EDc\u00ED Zm-p60.1 pod konstitutivn\u00EDm a inducibiln\u00EDm promotorem." . "beta-glukosid\u00E1za" . . "Subcellular compartmentation of reversible O-glucosylation in the homeostasis of active cytokinin levels"@en . "Subcellular compartmentation of the cytokinin (CK) metabolic network is crucial in determining levels of active hormone. Over-expression of Zm-p60.1, a maize b-glucosidase capable of releasing active CK from O-glucosides, leads to a disruption of zeatin metabolism. This protein can be used as a tool to understand subcellular compartmentation of reversible conjugation. Analysis of the phenotypes of transgenic tobacco plants over-expressing different subcellular variants of Zm-p60.1 is likely to yield crucial information regarding the mechanisms of homeostasis - at the cellular level - of active CK levels. Crosses between transgenic plants over-expressing Zm-p60.1 and those over-expressing the Phaseolus zeatin-O-glucosyltransferase ZOG1, and other crosses we have available will be analyzed with the objective of elucidating the processes regulating reversible CK conjugation. To overcome the problems associated with constitutive overexpression, and lack of suitable markers, transgenic Arabidopsis with inducible over-expression will be constructed and their phenotypes analyzed."@en . "beta-glukosid\u00E1za; metabolismus cytokinin; ektopick\u00E1 exprese; konjugace hormon\u016F; vnitrobun\u011B\u010Dn\u00E1 kompar"@en .