"Neuvedeno."@en . . "0"^^ . "GP204/03/D031" . "Apopt\u00F3za je d\u016Fle\u017Eit\u00FD fyziologick\u00FD proces, kter\u00FD p\u0159edstavuje p\u0159irozenou cestu z\u00E1niku bu\u0148ky. P\u0159i procesu permeabilizace mitochondri\u00E1ln\u00ED membr\u00E1ny b\u011Bhem apopt\u00F3zy se vn\u011Bj\u0161\u00ED mitochondri\u00E1ln\u00ED membr\u00E1na st\u00E1v\u00E1 propustnou pro proteiny v\u010Detn\u011B cytochromu c a apopt\u00F3zu vyvol\u00E1vaj\u00EDc\u00EDho faktoru (apoptosis-inducing factor, AIF). Po stimulaci apopt\u00F3zy se AIF dost\u00E1v\u00E1 do cytoplazmy a pot\u00E9 do j\u00E1dra, kde zp\u016Fsobuje kondenzaci chromatinu a hrubou (~50 kbp) DNA fragmentaci. D\u016Fsledek p\u016Fsoben\u00ED AIF na celkovou strukturu jadern\u00E9ho chromatinu a chromozomov\u00FDch teritori\u00ED nen\u00ED dosud zn\u00E1m a vhodn\u011B zvolen\u00E9 experimenty mohou p\u0159in\u00E9st nov\u00E9 poznatky o povaze tohoto p\u016Fsoben\u00ED. Pozornost bude tak\u00E9 v\u011Bnov\u00E1na studiu uvol\u0148ov\u00E1n\u00ED AIF, cytochromu c a zm\u011Bn\u00E1m v mitochondri\u00E1ln\u00EDm membr\u00E1nov\u00E9m potenci\u00E1lu, kter\u00E9 proces uvol\u0148ov\u00E1n\u00ED doprov\u00E1zej\u00ED. Z\u00EDskan\u00E9 poznatky roz\u0161\u00ED\u0159\u00ED nejen pochopen\u00ED funkce a regulace AIF, ale tak\u00E9 cel\u00E9ho apoptotick\u00E9ho procesu a mohou tak pomoci v\u00FDvoji nov\u00FDch terapeutick\u00FDch postup\u016F pro l\u00E9\u010Dbu z\u00E1va\u017En\u00FDch chorob, jak\u00FDmi jsou autoimunitn\u00ED," . . . "Apopt\u00F3zu vyvol\u00E1vaj\u00EDc\u00ED faktor (AIF): Jeho uvoln\u011Bn\u00ED z mitochondrie a zm\u011Bny, kter\u00E9 vyvol\u00E1v\u00E1 ve struktu\u0159e jadern\u00E9ho chromatinu" . "0"^^ . . "2007-10-16+02:00"^^ . . . . . . . . . "We studied the 3D structure of chromatin, which undergoes degradation during the apoptosis. We successfully tested the liposomal method to transport small particles into the cell and we used the precise video microscopy of their random motion to study th"@en . . . . "http://www.isvav.cz/projectDetail.do?rowId=GP204/03/D031"^^ . "Apoptosis is an important physiological process, that represents a natural way of the cell death. During the permeabilization of mitochondrial membrane in apoptosis, the outer mitochondrial membrane becomes soluble to proteins including cytochrome c and apoptosis-inducing factor, AIF. After a stimulation of apoptosis, AIF translocates into the cytoplasm and then into the cell nucleus, where it causes the chromatine condensation and large (-50 kbp) DNA fragmentation. The consequences of AIF action on thestructure of cell nucleus and on the structure of chromosomal territories are still unknown. Our innovative experiments should lead to new results further defining AIF action in cell nucleus. We will also study the mechanism of translocation of AIF and cytochrome c from mitochondria and connection of this mechanism with changes in mitochondrial membrane potential during apoptosis. Obtained results will widen not only our understanding of AIF action and regulation, but also our understanding of whole"@en . "10"^^ . . . . "Apoptosis-inducing factor (AIF): its translocation from mitochondria and its action on nuclear chromatin"@en . "Zab\u00FDvali jsme se studiem 3D struktury chromatinu, kter\u00FD se degraduje b\u011Bhem apopt\u00F3zy. Osv\u011Bd\u010Dila se metoda lipozom\u00E1ln\u00EDho transportu mal\u00FDch \u010D\u00E1stic do bu\u0148ky a n\u00E1sledn\u00E1 p\u0159esn\u00E1 videomikroskopie jejich samovoln\u00E9ho pohybu. Studovali jsme tak\u00E9 vlastnosti protein\u016F"@cs . . "1"^^ . . "10"^^ . .