"Strukturn\u02C7 studie proteinu p12 Mason-Pfizerova opi\u017A\u02C7ho viru pomoc\u02C7 NMR spektroskopie" . . "1"^^ . "Odborn\u011B p\u00FD\u02C7nos projektu lze spat\u00FDovat v objasn\u0158n\u02C7 p\u00FD\u02C7\u017Ain tvorby uspo\u00FD\u00A0dan\u201A struktury proteinu P12. \u00E9daje \u00FDe\u00E7itele jsou zcela adekv\u00A0tn\u02C7. V\u011Bznam projektu nem\u00A0 p\u00FD\u02C7mou aplikaci, pova\u00A7uji jej v\u00E7ak za p\u00FD\u02C7nos k pozn\u00A0n\u02C7 v\u011Bznamu vy\u00E7\u00E7\u02C7ch b\u02C7lkovinn\u011Bch struktur. V\u011Bs"@cs . . . "0"^^ . "1"^^ . . . "Neuvedeno."@en . . "During the maturation of Mason-Pfizer monkey virus polyprotein Gag precursor is cleaved by protease yielding six proteins: p10 (matrix protein - MA), pp24/18, p12, p27 (capsid protein - CA), p14 (nucleocapsid protein - NC) and p4. The functions of pp24/18, p12 and p4 proteins have not been sufficiently explained yet. The deletion mutation experiments in gene encoding protein p12 proved the hypothesis, that this protein supports the assembly of immature viral capsid. The aim of this project is to solve three-dimensional structure of N-terminal domain of protein p12, that could be utilized for a design of suitable inhibitor or viral capsid assembly. The protein will be expressed in E. coli BL21(DE3). Multidimensional NMR spectroscopy on isotopically labeled (15N or 15N,13C) samples together with computational methods will be employed for the solution of three-dimensional structure of this protein."@en . "GP203/02/P098" . . . . . . "2009-06-15+02:00"^^ . "Polyproteinov\u011B prekurzor Gag Mason-Pfizerova opi\u017A\u02C7ho viru je b\u0158hem maturace roz\u00E7t\u0158pen proteasou na \u00E7est protein\u2026: p10 (matrixov\u011B protein - MA), pp24/18, p12, p27 (kapsidov\u011B protein - CA), p14 (nukleokapsidov\u011B protein - NC) a p4. Funkce protein\u2026 pp24/18, p12 a p4 dosud nebyla uspokojiv\u0158 vysv\u0158tlena. Metodou dele\u017An\u02C7ch mutagenez\u02C7 v genu k\u02D8duj\u02C7c\u02C7m protein p12 bylo prok\u00A0z\u00A0no, \u00A7e tento protein napom\u00A0h\u00A0 skl\u00A0d\u00A0n\u02C7 nezral\u201A virov\u201A kapsidy. C\u02C7lem tohoto projektu je vy\u00FDe\u00E7it trojdimenzion\u00A0ln\u02C7 strukturu N-termin\u00A0ln\u02C7 dom\u201Any proteinu p12, na jej\u02C7m\u00A7 z\u00A0klad\u0158 bude mo\u00A7n\u201A navrhnout vhodn\u011B inhibitor skl\u00A0d\u00A0n\u02C7 virov\u201A kapsidy. Pro p\u00FD\u02C7pravu proteinu bude pou\u00A7ita metoda exprese v E.coli BL21(DE3). K \u00FDe\u00E7en\u02C7 prostorov\u201A struktury proteinu p12 bude vyu\u00A7ita v\u02C7cedimenzion\u00A0ln\u02C7 NMR spektroskopie izotopov\u0158 obohacen\u011Bch (15N nebo 15N, 13C) vzork\u2026 spolu s v\u011Bpo\u017Aetn\u02C7mi metodami." . . . "http://www.isvav.cz/projectDetail.do?rowId=GP203/02/P098"^^ . "0"^^ . . . . "Structural study of protein p12 from Mason-Pfizer monkey virus using NMR spectroscopy"@en . "1"^^ .