. . . . "The grant project is devoted to the study on the impact of chemical modifications on structural and hybridization properties of synthetic analogues of nucleic acids. Main attention will be paid to finding of an optimal modification leading to thestabilization of double and triple helical structures by means of inter-strand interaction. This post-doc project is attached to the main project within the framework of which the enzymatic stable phosphonate nucleotide analogues will be prepared.Nucleic acids with improved hybridization and enzymatic stability properties are interesting from the point of view of potential chemotherapeutic utilization by means of %22antisense%22 mechanism (modified nucleic acid strand bind to a specific sequence ofnucleotides in its mRNA target to inhibit production of the specific protein). The theoretical approach will be based on molecular dynamics (MD) technique, which allows simulations at non-zero temperature (typically 300 K), with atomic representation of"@en . . "http://www.isvav.cz/projectDetail.do?rowId=GP202/02/D114"^^ . . "Molecular Dynamics Simulations of Modified Nucleic Acids - Potential Chemoterapeutics"@en . . "Projekt je zam\u011B\u0159en na studium vlivu chemick\u00E9 modifikace na strukturn\u00ED a hybridiza\u010Dn\u00ED vlastnosti syntetick\u00FDch analog nukleov\u00FDch kyselin. Hlavn\u00ED pozornost bude v\u011Bnov\u00E1na nalezen\u00ED optim\u00E1ln\u00ED modifikace vedouc\u00ED ke stabilizaci dvoj\u0161roubovicovit\u00FDch atroj\u0161roubovicovit\u00FDch struktur prost\u0159ednictv\u00EDm intervl\u00E1knov\u00E9 interakce. Tento postdoktorandsk\u00FD projekt je p\u0159idru\u017Een k nosn\u00E9mu projektu v jeho\u017E r\u00E1mci jsou p\u0159ipravov\u00E1na enzymaticky stabiln\u00ED fosfon\u00E1tov\u00E1 analoga nukleov\u00FDch kyselin. Nukleov\u00E9 kyseliny sezv\u00FD\u0161en\u00FDmi schopnostmi hybridizace a stability v\u016F\u010Di nukle\u00E1z\u00E1m jsou zaj\u00EDmav\u00E9 z hlediska jejich vyu\u017Eit\u00ED jako potenci\u00E1ln\u00EDch chemoterapeutik funguj\u00EDc\u00EDch prost\u0159ednictv\u00EDm %22antisense%22 mechanismu (modifikovan\u00E9 vl\u00E1kno nukleov\u00E9 kyseliny se v\u00E1\u017Ee ke specifick\u00E9sekvenci nukleotid\u016F v mRNAa inhibuje tak tvorbu n\u011Bjak\u00E9ho specifick\u00E9ho proteinu). Metoda \u0159e\u0161en\u00ED bude zalo\u017Eena na molekul\u00E1rn\u011B-dynamick\u00FDch simulac\u00EDch, kter\u00E9 dovoluj\u00ED simulace p\u0159i nenulov\u00E9 teplot\u011B (typicky 300 K) s atom\u00E1rn\u00ED reprezentac\u00ED roztoku a iont\u016F." . . . . . . . "7"^^ . "2008-05-19+02:00"^^ . "7"^^ . . "Molekul\u00E1rn\u011B-dynamick\u00E9 simulace modifikovan\u00FDch nukleov\u00FDch kyselin - potenci\u00E1ln\u00EDch chemoterapeutik" . . "1"^^ . . "Molecular dynamics simulations answered the question how the positive charges stabilize the duplex and triplex structures consisting of natural or a-anomeric strands. This revealed that amino- / guanidium-alkyl tethers are able to bridge nucleic acids gr"@en . . . "GP202/02/D114" . "Molekul\u00E1rn\u011B-dynamick\u00E9 simulace zodpov\u011Bd\u011Bly ot\u00E1zku, jak\u00FDm zp\u016Fsobem mohou skupiny atom\u016F s\u00A0kladn\u00FDm n\u00E1bojem stabilizovat dvoj\u0161roubovicovit\u00E9 a troj\u0161roubovicovit\u00E9 struktury nukleov\u00FDch kyselin sest\u00E1vaj\u00EDc\u00ED z\u00A0p\u0159irozen\u00FDch \u010Di a-anomerick\u00FDch vl\u00E1ken nukleov\u00FDch kyseli"@cs . . "0"^^ . . . "Neuvedeno."@en . "0"^^ .