. . "N-methyl-D-aspart\u00E1tov\u00E9 (NMDA) receptory zprost\u0159edkov\u00E1vaj\u00ED rychl\u00FD excita\u010Dn\u00ED synaptick\u00FD p\u0159enos v centr\u00E1ln\u00ED nervov\u00E9 soustav\u011B savc\u016F. Jejich aktivace je d\u016Fle\u017Eit\u00E1 pro rozvoj mozku, synaptickou plasticitu a u\u010Den\u00ED, ale z\u00E1rove\u0148 m\u016F\u017Ee b\u00FDt v\u00FDznamn\u00FDm p\u0159isp\u011Bvatelem p\u0159i rozvoji neurodegenerativn\u00EDch proces\u016F. C\u00EDlem tohoto projektu je objasnit mechanizmy podmi\u0148uj\u00EDc\u00ED transport NMDA receptor\u016F na bun\u011B\u010Dn\u00FD povrch. Pomoc\u00ED metod molekul\u00E1rn\u00ED biologie, biochemie, imunocytochemie a elektrofyziologie i) identifikujeme podjednotkov\u011B-v\u00E1zan\u00E9 mechanizmy reguluj\u00EDc\u00ED transport receptor\u016F a ii) objasn\u00EDme zda je uvoln\u011Bn\u00ED receptoru z endoplazmatick\u00E9ho retikula v\u00E1z\u00E1no na konkr\u00E9tn\u00ED funk\u010Dn\u00ED stav. \u0158e\u0161en\u00ED tohoto projektu p\u0159inese nov\u00E9 poznatky o molekul\u00E1rn\u00EDch a funk\u010Dn\u00EDch vlastnostech NMDA receptor\u016F, ketr\u00E9 mohou b\u00FDt d\u00E1le vyu\u017Eity p\u0159i hled\u00E1n\u00ED nov\u00FDch mo\u017Enost\u00ED l\u00E9\u010Dby neurodegenerativn\u00EDch onemocn\u011Bn\u00ED." . . " ion channel" . " surface expression" . . . "GP14-09220P" . . . "NMDA receptor, ion channel, endoplasmic reticulum, transport, surface expression, kinetics"@en . "2015-02-09+01:00"^^ . . " endoplasmic reticulum" . "NMDA receptor" . "2014-01-01+01:00"^^ . "N-methyl-D-aspartate (NMDA) receptors mediate fast excitatory synaptic transmission in the mammalian central nervous system. Their activation plays a key role in brain development, synaptic plasticity, memory formation, but it is also major contributor to excitotoxic neurodegeneration. The main objective of this project is to elucidate mechanisms determining trafficking of NMDA receptors. Using a multidisciplinary approach that includes molecular biology, biochemistry, immunocytochemistry and electrophysiology methods we will i) identify subunit-specific mechanisms that regulate intracellular processing and surface delivery of NMDA receptors and ii) elucidate whether any functional state of the NMDA receptor is preferentially released from the endoplasmic reticulum. The results of this proposal will bring new findings about molecular and functional properties of NMDA receptors that could be in future utilized for new strategies in the treatment of neurodegeneration."@en . . . "0"^^ . . . . "2016-12-31+01:00"^^ . "1"^^ . "Structural and functional determinants of NMDA receptor trafficking"@en . "0"^^ . "2"^^ . "2"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=GP14-09220P"^^ . . "2014-04-07+02:00"^^ . . . . "Strukturn\u00ED a funk\u010Dn\u00ED determinanty transportu NMDA receptor\u016F" . " transport" .