"http://www.isvav.cz/projectDetail.do?rowId=GP13-32339P"^^ . "Lon proteases are the most flexible ATP-dependent proteases, which are both in prokaryotic and eukaryotic cells responsible for protein degradation, regulate levels of transcription factors and supposedly participate in apoptosis. Malfunctions of these proteases are linked with dystrophies, neurodegenerative diseases and cancer. A deeper insight into their mechanisms of action requires detailed knowledge of their native three-dimensional (3D) structure in the course of its main functional cycle, the ATP-ase cycle. 3D structure of any flexible Lon protease complex has not been determined so far, however, human mitochondrial Lon protease is the most stable Lon protease purified so far, and therefore we will employ conventional and cryo-electron microscopy in connection with the single particle analysis method to determine the 3D structure of the wild-type of human Lon in the course of its ATP-ase cycle, i.e. in interactions with Mg2+ cations, ATP, ADP and protein substrate, as well as of a few of its available mutant forms, with expected resolutions of ~1nm."@en . . "2015-02-09+01:00"^^ . "Lon prote\u00E1zy jsou strukturn\u011B nejflexibiln\u011Bj\u0161\u00ED ATP-z\u00E1visl\u00E9 prote\u00E1zy, je\u017E jsou v bu\u0148k\u00E1ch prokaryot i eukaryot zodpov\u011Bdn\u00E9 za degradaci po\u0161kozen\u00FDch protein\u016F, udr\u017Euj\u00ED hladinu kr\u00E1tkodob\u011B existuj\u00EDc\u00EDch b\u00EDlkovin a p\u0159edpokl\u00E1d\u00E1 se jejich \u00FA\u010Dast v apopt\u00F3ze. Poruchy ve funkci t\u011Bchto prote\u00E1z jsou spojov\u00E1ny s dystrofiemi, neurodgenerativn\u00EDmi onemocn\u011Bn\u00EDmi a rakovinou. Hlub\u0161\u00ED porozum\u011Bn\u00ED jejich funk\u010Dn\u00EDm mechanism\u016Fm vy\u017Eaduje znalost jejich nativn\u00ED trojrozm\u011Brn\u00E9 (3D) struktury v pr\u016Fb\u011Bhu jejich hlavn\u00EDho funk\u010Dn\u00EDho cyklu, tzv. ATP-cyklu. 3D strukturu \u017E\u00E1dn\u00E9 Lon prote\u00E1zy se v\u0161ak zat\u00EDm nepoda\u0159ilo ur\u010Dit, lidsk\u00E1 mitochondri\u00E1ln\u00ED Lon prote\u00E1za je v\u0161ak dosud nejstabiln\u011Bj\u0161\u00ED izolovanou Lon prote\u00E1zou, a proto konven\u010Dn\u00ED a kryo-elektronovou mikroskopi\u00ED ve spojen\u00ED s metodami single-particle anal\u00FDzy (SPA) ur\u010D\u00EDme strukturu wild-type Lon v pr\u016Fb\u011Bhu jej\u00EDho ATP-cyklu, tj. p\u0159i interakci s kationty Mg2+, ATP, ADP a proteinov\u00FDm substr\u00E1tem, a strukturu n\u011Bkolika mutantn\u00EDch forem s p\u0159edpokl\u00E1dan\u00FDm rozli\u0161en\u00EDm ~1 nm." . . " mitochondria" . . "0"^^ . . . " (cryo-) transmission electron microscopy" . "2014-04-07+02:00"^^ . "GP13-32339P" . "Human mitochondrial Lon protease" . "Pohled do dynamiky ATP-dependentn\u00ED lidsk\u00E9 mitochondri\u00E1ln\u00ED Lon prote\u00E1zy trojrozm\u011Brnou elektronov\u011B mikroskopickou strukturn\u00ED anal\u00FDzou" . "Insights into the dynamics of the ATP-dependent human mitochondrial Lon protease by its three-dimensional electron microscopic structural analysis"@en . . " single particle analysis" . . . "2015-12-31+01:00"^^ . . . "3"^^ . . "0"^^ . . . . "3"^^ . . . "1"^^ . . "Human mitochondrial Lon protease, mitochondria, (cryo-) transmission electron microscopy, single particle analysis, 3D reconstruction"@en . "2013-02-01+01:00"^^ . .