"0"^^ . . "1"^^ . . . "1"^^ . "0"^^ . "1"^^ . . . "Anal\u00FDza diversity metabolicky aktivn\u00EDch bakteri\u00ED v kontaminovan\u00E9 zemin\u011B s vyu\u017Eit\u00EDm isotopov\u00E9ho zna\u010Den\u00ED a metagenomiky" . "microbial diversity" . . . . "GP13-20414P" . . "2013-02-01+01:00"^^ . . "microbial diversity, metabolic activity, bioremediation, stable isotope probing, metagenomics"@en . . " bioremediation" . . " stable isotope probing" . . "P\u0159esn\u00E9 posouzen\u00ED mikrobi\u00E1ln\u00ED diversity a aktivity a jej\u00EDch reakc\u00ED na p\u016Fsoben\u00ED r\u016Fzn\u00FDch faktor\u016F je jedn\u00EDm z hlavn\u00EDch \u00FAkol\u016F mikrobi\u00E1ln\u00ED ekologie. V r\u00E1mci p\u0159edkl\u00E1dan\u00E9ho projektu budou pomoc\u00ED metod isotopov\u00E9ho zna\u010Den\u00ED DNA a metagenomiky studov\u00E1ny mikrobi\u00E1ln\u00ED komunity a jejich funk\u010Dn\u00ED geny v zemin\u011B kontaminovan\u00E9 polychlorovan\u00FDmi bifenyly (PCB). Hlavn\u00EDm c\u00EDlem je popsat, jak zm\u011Bny ve struktu\u0159e mikrobi\u00E1ln\u00EDch komunit (v p\u0159\u00EDtomnosti halofytn\u00EDch rostlin, v p\u0159ebytku soli a po bioaugmentaci) koreluj\u00ED s metabolickou aktivitou a pota\u017Emo s odbour\u00E1v\u00E1n\u00EDm PCB ze zeminy. Vedle diversity fylogenetick\u00E9 bude analyzov\u00E1na i funk\u010Dn\u00ED diversita dioxygenas, kter\u00E9 se pod\u00EDlej\u00ED na metabolismu bifenylu a PCB. Experiment umo\u017E\u0148uje t\u00E9\u017E sledovat v\u00FDvoj diversity t\u011Bchto dioxygenas bezprost\u0159edn\u011B po kontaminaci zeminy PCB za \u00FA\u010Delem lep\u0161\u00EDho porozum\u011Bn\u00ED vtah\u016Fm mezi prim\u00E1rn\u00ED strukturou a substr\u00E1tovou specificitou t\u011Bchto enzym\u016F." . . . . . "Accurate assessment of microbial diversity and activity and their responses to different stimuli is one of the central goals of microbial ecology. This project aims to employ DNA-stable isotope probing (SIP) and metagenomics to extensively study microbial communities and their functional genes in polychlorinated biphenyl (PCB)-contaminated soil. The overall aim is to discover how shifts in microbial community structure (upon the presence of halophytic plant species, excess of salt, and bioaugmentation) correlate with changes in metabolic activity and hence the removal of PCBs from the soil. In addition to phylogenetic diversity, functional diversity will be analyzed of the genes encoding for dioxygenases involved in the biodegradation of biphenyl and PCBs. The planned experimental design also has an advantage that it will monitor the diversity and abundance of these dioxygenases in response to contamination of a pristine soil. This will help further relate the primary structure of these enzymes with the affinity towards PCBs."@en . "Diversity analysis of metabolically active bacteria in contaminated soil using stable isotope probing and metagenomics"@en . " metabolic activity" . "2015-02-09+01:00"^^ . . "2014-04-07+02:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=GP13-20414P"^^ . "2015-12-31+01:00"^^ .