"Pospiviroidn\u00ED patogeneze jako regula\u010Dn\u00ED porucha zprost\u0159edkovan\u00E1 mal\u00FDmi viroid-specifick\u00FDmi RNA." . . . "2014-01-27+01:00"^^ . "V\u00A0projektu budeme analyzovat mechanismy patogeneze dvou viroid\u016F (mal\u00FDch infek\u010Dn\u00EDch, jedno\u0159et\u011Bzcov\u00FDch, cirkul\u00E1rn\u00EDch, nek\u00F3duj\u00EDc\u00EDch a autonom\u011B se replikukj\u00EDc\u00EDch molekul RNA) z\u00A0\u010Deledi Pospiviroidae: viroidu v\u0159etenovitosti hl\u00EDz bramboru (PSTVd)-choroby \u0159ady plodin, druh\u016F zeleniny i okrasn\u00FDch rostlin a expanzivn\u00EDho viroidu zakrslosti chmele (HSVd), nebezpe\u010Dn\u00E9ho zejm\u00E9na\u00A0 pro p\u011Bstov\u00E1n\u00ED chmele v\u00A0Evrop\u011B. Na z\u00E1klad\u011B na\u0161eho p\u0159ede\u0161l\u00E9ho studia chceme d\u00E1le ov\u011B\u0159it hypot\u00E9zu, \u017Ee viroidn\u00ED patogeneze zahrnuje tvorbu mal\u00FDch viroid-specifick\u00FDch (vs) RNA, jejich\u017E interakce vedou k\u00A0poruch\u00E1m regulace tzv. mikro RNA a transkrip\u010Dn\u00EDch regula\u010Dn\u00EDch faktor\u016F (TF) hostitele. Pro anal\u00FDzu t\u011Bchto mechanism\u016F provedeme 1. tzv. \u201Ehlubok\u00E9\u201C sekvenv\u00E1n\u00ED mal\u00FDch molekul RNA; 2. predickci c\u00EDlov\u00FDch molekul pro vsRNA in silico zejm\u00E9na d\u00EDky srovn\u00E1n\u00ED s\u00A0v\u00FDsledky dostupn\u00E9ho celogenomov\u00E9ho sekvenov\u00E1n\u00ED genotypu raj\u010Dete citliv\u00E9ho k\u00A0PSTVd; 3. ov\u011B\u0159en\u00ED\u00A0\u00A0molekul\u00E1rn\u00EDch c\u00EDl\u016F vsRNA kvantitativn\u00EDmi metodami anl\u00FDzy exprese RNA, TF, promotorov\u00E9 aktivity a technologi\u00ED viry indukovan\u00E9 suprese gen\u016F (VIGS) u druhu N. benthamiana, raj\u010Dete a chmelu.\u00A0\u00A0\u00A0" . . " gene" . . . "0"^^ . " H." . "Final report is carefully written and contains all required information. The project was executed at high level and lead to novel results on pathogenic effects of viroids and a multitargeting model of pathogenesis was confirmed that leads to aberrant function of regulatory networks. The results were published in 3 scientific journals with impact factor and on several scientific conferences."@en . "Z\u00E1v\u011Bre\u010Dn\u00E1 zpr\u00E1va je pe\u010Dliv\u011B vypracov\u00E1na a obsahuje v\u0161echny n\u00E1le\u017Eitosti. Projekt byl \u0159e\u0161en na v\u00FDborn\u00E9 \u00FArovni a byly z\u00EDsk\u00E1ny p\u016Fvodn\u00ED poznatky o patogenn\u00EDm \u00FA\u010Dinku viroid\u016F a byla potvrzena hypot\u00E9za o mnoho\u010Detn\u00E9m c\u00EDlen\u00ED molekul, kter\u00E9 vede k poruch\u00E1m regula\u010Dn\u00EDch s\u00EDt\u00ED. V\u00FDsledky projektu byly zve\u0159ejn\u011Bny ve 3 v\u011Bdeck\u00FDch \u010Dasopisech s impakt faktorem a na \u0159ad\u011B v\u011Bdeck\u00FDch konferenc\u00ED."@cs . . . "1"^^ . . "Pospiviroid pathogenesis as \u201Cregulatory disorder\u201C mediated by viroid-specific small RNAs."@en . . " silencing" . "0"^^ . " factors" . "viroid; viroid; disease; gene; silencing; miRNA; transcription; factors; H.; lupulus; S.; lycopersicum"@en . "2012-03-30+02:00"^^ . " miRNA" . . . . " lupulus" . "GCP501/10/J018" . "9"^^ . . "9"^^ . . . . "viroid" . . . "\u00A0\u00A0 We will analyze mechanisms of pathogenesis of two viroids (small, infectious, single-stranded, circular, non-coding and autonomously replicating RNAs) from Pospiviroidae family: potato spindle tuber viroid (PSTVd) infecting various crops, vegetables and ornamentals and highly expansive hop stunt viroid (HSVd) that, in particular, represents a danger to hops in Europe. Based on our previous study, we aim to verify the hypothesis that pathogenesis caused by pospiviroids involve viroid-specific (vs) small RNAs, mis-regulation of host-encoded microRNAs and plant transcription factors (TFs).\u00A0To analyze the mechanism(s) of viroid pathogenesis we perform: 1. \u201Cdeep\u201D sequencing of small RNAs; 2. prediction of vsRNA targets in silico including the whole genome data of\u00A0 PSTVd-sensitive tomato recently available; 3. verification of vsRNA molecular targets, components of silencing mechanisms and TFs involved in pospiviroid pathogenesis by quantitative RNA expression assays, promoter activity assays and by virus-induced gene silencing technology (VIGS) using N. benthamiana, tomato and hop."@en . . " S." . . " transcription" . " viroid" . "2010-01-01+01:00"^^ . " disease" . "http://www.isvav.cz/projectDetail.do?rowId=GCP501/10/J018"^^ . "2012-12-31+01:00"^^ .