. "2014-05-16+02:00"^^ . "2015-04-23+02:00"^^ . "GAP506/11/1320" . "0"^^ . . "Projekt je zam\u011B\u0159en na v\u00FDzkum euglen - vhodnou skupinu pro studium vzniku sekund\u00E1rn\u00EDho plastidu. Budeme p\u00E1trat po zelen\u00E9 \u0159ase, kter\u00E1 je je\u0161t\u011B p\u0159\u00EDbuzn\u011Bj\u0161\u00ED p\u0159edku euglenid\u00EDho plastidu ne\u017E dosud zn\u00E1m\u00FD druh Pyramimonas parkeae. Metodou 454 osekvenujeme transkriptom (cDNA) t\u00E9to zelen\u00E9 \u0159asy nebo P. parkeae a t\u0159\u00ED druh\u016F euglen Eutreptiella gymnastica, Phacus caudatus a nefotosyntetick\u00E9 eugleny Rhabdomonas sp. U fotosyntetick\u00FDch druh\u016F euglen a u zelen\u00E9 \u0159asy osekvenujeme tak\u00E9 plastidov\u00FD genom. Ze z\u00EDskan\u00FDch dat se pokus\u00EDme rekonstruovat proteomy plastid\u016F a provedeme srovn\u00E1vac\u00ED anal\u00FDzu plastidov\u00FDch genom\u016F a proteom\u016F (v\u010Detn\u011B studovan\u00E9ho plastidu E. gracilis). Budeme si v\u0161\u00EDmat, jak podobn\u00E9 \u010Di odli\u0161n\u00E9 jsou plastidy, kter\u00E9 se etablovaly ve t\u0159ech lini\u00EDch euglen. Budeme analyzovat tak\u00E9 fylogenetick\u00FD p\u016Fvod protein\u016F v plastidu i cytosolu u jednotliv\u00FDch euglen a zaj\u00EDmat n\u00E1s bude p\u0159edev\u0161\u00EDm p\u0159\u00EDsp\u011Bvek gen\u016F poch\u00E1zej\u00EDc\u00EDch ze sinice (p\u0159edka prim\u00E1rn\u00EDho plastidu) a zelen\u00E9 \u0159asy (endosymbionta). Srovn\u00E1me p\u0159\u00EDsp\u011Bvky endosymbi\u00F3zy do proteomu r\u016Fzn\u00FDch lini\u00ED euglenid\u016F." . . "Establishment of the secondary plastid in euglenids"@en . . . "1"^^ . . . "1"^^ . . . "2015-12-31+01:00"^^ . . "4"^^ . "2011-01-01+01:00"^^ . "Vznik sekund\u00E1rn\u00EDho plastidu u euglenid\u016F" . "4"^^ . . . . . . . . "secondary endosymbiosis endosymbiotic gene transfer euglenids plastid"@en . "http://www.isvav.cz/projectDetail.do?rowId=GAP506/11/1320"^^ . . "The project is focused on the secondary plastids of euglenids. We will perform environmental survey in order to reveal a green alga related to the ancestor of the euglenid plastid closer than the so far known Pyramimonas parkeae. Using 454 technique we will sequence the transcriptome (cDNA) of this alga or P. parkeae and three species of euglenids -\u00A0 Eutreptiella gymnastica, Phacus caudatus a non-photosynthetic Rhabdomonas sp. Furthermore we will sequence the plastid genome of the alga and photosynthetic euglenids.\u00A0 From the data we will reconstruct plastid proteomes and perform a comparative analysis including the well studied plastid of E. gracilis. We will notice the similarities and differences between genomes and proteomes of plastid established in three lineages of euglenids. We will be interested in the phylogenetic origin of proteins of euglenids, particularly in the contribution of proteins originating from the cyanobacteria (ancestor of the primary plastid) and the green alga (endosymbiot). The contributions will be compared between the euglenid lineages."@en .