" complement" . "http://www.isvav.cz/projectDetail.do?rowId=GAP506/10/2136"^^ . . " Borrelia" . "Vynikaj\u00EDc\u00ED v\u00FDsledky ukazuj\u00ED na dosud m\u00E1lo zn\u00E1m\u00E9 faktory imunitn\u00EDho syst\u00E9mu kl\u00ED\u0161\u0165at (pr\u016Fkaz thioesterov\u00FDch protein\u016F, inhibitor\u016F peptid\u00E1z, konvert\u00E1z; fungov\u00E1n\u00ED primitivn\u00ED komplementov\u00E9 kask\u00E1dy, testov\u00E1n\u00ED funkce protein\u016F v hemocytech pomoc\u00ED RNAi, atd.). Poznatky mohou m\u00EDt z\u00E1sadn\u00ED v\u00FDznam pro pochopen\u00ED p\u0159enosu patogen\u016F. V\u00FDstupem jsou 3 impaktovan\u00E9 publikace a n\u011Bkolik dal\u0161\u00EDch v p\u0159\u00EDprav\u011B."@cs . . . . "6"^^ . . "6"^^ . . . . "2012-03-30+02:00"^^ . "2014-01-30+01:00"^^ . "2012-12-31+01:00"^^ . . "Functional genomics of the complement-like molecules in the tick Ixodes ricinus"@en . . . "0"^^ . "2010-01-01+01:00"^^ . "Current release of the tick Ixodes scapularis genome and feasibility of RNA interference (RNAi) promote ticks to become a full-value model for deeper studies of tick\u00A0immune system and its interaction with transmitted pathogens.\u00A0Previously, we have characterized tick alpha2-macroglobulins (a2M) and fibrinogen-related lectins playing an\u00A0important role in invertebrate immunity. Data mining\u00A0from I. scapularis\u00A0genome further revealed 9 proteins related to a2M, C3 complement component, insect thioester proteins and a homolog of C2/factor B. Given the close similarity of the European tick Ixodes ricinus, we can perform a reasonable functional genomics study based on RNAi silencing of tick complement-like molecules linked with reliable phagocytic assay of model pathogen Chryseobacterium indologenes\u00A0by tick hemocytes. These experiments will disclose which molecules are involved and how they are organized in an ancient complement-like pathway.\u00A0The cellular immune response will be tested also against other bacteria including the Lyme disease spirochetes."@en . . "Sou\u010Dasn\u00E9 zve\u0159ejn\u011Bn\u00ED genomu kl\u00ED\u0161t\u011Bte Ixodes scapularis a mo\u017Enost pou\u017Eit\u00ED RNA interference (RNAi) umo\u017E\u0148uje, aby se kl\u00ED\u0161\u0165ata stala plnohodnotn\u00FDm modelem pro detailn\u00ED zkoum\u00E1n\u00ED interakce jejich imunitn\u00EDho syst\u00E9mu s p\u0159en\u00E1\u0161en\u00FDmi patogeny. Ji\u017E d\u0159\u00EDve jsme charakterizovali kl\u00ED\u0161t\u011Bc\u00ED alfa2-makroglobuliny a fibrinogenu-podobn\u00E9 lektiny, kter\u00E9 hraj\u00ED\u00A0d\u016Fle\u017Eitou \u00FAlohu v imunit\u011B bezobratl\u00FDch. Hled\u00E1n\u00EDm v genomu I. scapularis\u00A0jsme d\u00E1le nalezli 9 dal\u0161\u00EDch\u00A0protein\u016F\u00A0p\u0159\u00EDbuzn\u00FDch a2M, C3 slo\u017Ece komplementu, hmyz\u00EDm thioesterov\u00FDm protein\u016Fm a homolog slo\u017Eky C2/ faktoru B.\u00A0D\u00EDky bl\u00EDzk\u00E9 p\u0159\u00EDbuznosti evropsk\u00E9ho kl\u00ED\u0161t\u011Bte Ixodes ricinus m\u016F\u017Eeme prov\u00E9st relevantn\u00ED funk\u010Dn\u011B genomickou studii zalo\u017Eenou na RNAi uml\u010Den\u00ED komplementu-podobn\u00FDch molekul ve spojen\u00ED se spolehliv\u00FDm testem fagocyt\u00F3zy modelov\u00E9ho patogena Chryseobacterium indologenes\u00A0kl\u00ED\u0161t\u011Bc\u00EDmi hemocyty. Tyto experimenty odhal\u00ED z\u00FA\u010Dastn\u011Bn\u00E9 molekuly a jejich\u00A0funkci v primitvn\u00ED dr\u00E1ze podobn\u00E9 komplementu.\u00A0Tato bun\u011B\u010Dn\u00E1 imunitn\u00ED odpov\u011B\u010F bude studov\u00E1na i proti dal\u0161\u00EDm bakteri\u00EDm v\u010Detn\u011B spirochet Lymsk\u00E9 boreli\u00F3zy." . "GAP506/10/2136" . "1"^^ . "Funk\u010Dn\u00ED genomika molekul podobn\u00FDch komplementu u kl\u00ED\u0161t\u011Bte Ixodes ricinus" . . "0"^^ . . " Ixodes" . . . . . "tick; Ixodes; Borrelia; complement; phagocytosis"@en . "Excellent results characterize poorly known factors of the tick immune system (thioester-containing proteins, peptidase inhibitors, convertases; primitive complement cascade, RNAi-proved function of selected proteins in hemocytes, etc.). These data can substantially influence our understanding of pathogen transmission via ticks. Outputs: 3 papers in international journals and several manuscripts."@en . . "tick" . .