"St\u00E1le je\u0161t\u011B nen\u00ED zn\u00E1m\u00E9, jak\u00FDm mechanismem n\u011Bkter\u00E9 l\u00E1tky \u017Eivotn\u00EDho prost\u0159ed\u00ED tzv. endokrinn\u00ED disruptory (EDs) ovliv\u0148uj\u00ED reprodukci \u017Eiv\u00FDch organism\u016F. V tomto projektu budeme na sav\u010D\u00EDm modelu, my\u0161i (inbredn\u00ED, outbredn\u00ED) a ryb\u00EDm modelu zji\u0161\u0165ovat epigenetick\u00E9 zm\u011Bny v reproduk\u010Dn\u00EDch org\u00E1nech po vystaven\u00ED EDs v podm\u00EDnk\u00E1ch in vivo. My\u0161i a ryby budou vystaveny b\u011Bhem embryon\u00E1ln\u00EDho v\u00FDvoje p\u016Fsoben\u00ED EDs v r\u016Fzn\u00FDch d\u00E1vk\u00E1ch a bude sledov\u00E1n p\u0159enos epigenetick\u00FDch poruch v gon\u00E1d\u00E1ch a z\u00E1rode\u010Dn\u00FDch bu\u0148k\u00E1ch v dal\u0161\u00EDch generac\u00EDch. Anal\u00FDza bude zam\u011B\u0159ena na zm\u011Bny DNA methylace and histonov\u00E9 posttransla\u010Dn\u00ED modifikace. Anal\u00FDza zm\u011Bn na \u00FArovni molekul a bun\u011Bk bude prov\u00E1d\u011Bna na spermi\u00EDch, spermatogoni\u00EDch a z\u00E1rode\u010Dn\u00FDch bu\u0148k\u00E1ch. Na \u00FArovni fenotypu bude hodnocena histopatologie sam\u010D\u00EDch gon\u00E1d a funk\u010Dn\u00ED anal\u00FDza spermi\u00ED. C\u00EDlem tohoto projektu bude zjistit a ov\u011B\u0159it biomarkery, kter\u00E9 zprost\u0159edkov\u00E1vaj\u00ED mezigenera\u010Dn\u00ED p\u0159enos sam\u010D\u00ED gonad\u00E1ln\u00ED dysfunkce jako d\u016Fsledek expozice vybran\u00FDch polutant\u016F u obou druh\u016F \u017Eivo\u010Dich\u016F." . "22"^^ . "0"^^ . . . "22"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GAP503/12/1834"^^ . . . "2014-04-28+02:00"^^ . . . "1"^^ . . "1"^^ . "2015-04-23+02:00"^^ . . . "Endocrine disruptors DNA-methylation histone post-transcriptional modifications microRNA expression Primordial Germ Cells spermatids spermatozoa histopathology"@en . "2015-12-31+01:00"^^ . . "GAP503/12/1834" . "The mechanism by which some environmental substances called endocrine disruptors (Eds) affect the reproduction of living organisms is still unknown. In this project we will identify the epigenetic changes in reproductive organs after exposure to EDs in vivo and in vitro in the mouse (inbred, outbred) and fish model. Mice and fish will be exposed during embryonic development to EDs at different doses and the transfer of epigenetic defects in the gonads and germ cells in future generations will be monitored. The analysis will focus on changes in DNA methylation and histone posttranslational modification. Analysis of changes in the level of molecules and cells will be carried out on spermatozoa and spermatogonial stem cells. At the level of phenotype changes will be assessed by histopathology of male gonads and sperm analysis. The aim of this project will be to identify and validate biomarkers that mediate inter-generational transfer of male gonadal dysfunction as a result of exposure to selected contaminants in both species."@en . . . . . . "2012-01-01+01:00"^^ . . . "Identification of epigenetic biomarkers of male germ cell disorders linked to adverse environmental factors"@en . "Identifikace epigenetick\u00FDch biomarker\u016F sam\u010D\u00EDch z\u00E1rode\u010Dn\u00FDch bun\u011Bk po\u0161kozen\u00FDch nep\u0159\u00EDzniv\u00FDmi vlivy \u017Eivotn\u00EDho prost\u0159ed\u00ED" . . .