. "2012-01-01+01:00"^^ . "2015-04-23+02:00"^^ . . "Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering"@en . "1"^^ . "Antropogenn\u00ED slou\u010Denina 1,2,3-trichlorpropan (TCP) je uvol\u0148ov\u00E1na do \u017Eivotn\u00EDho prost\u0159ed\u00ED v d\u016Fsledku v\u00FDroby a pou\u017Eit\u00ED jako rozpou\u0161t\u011Bdlo a extrak\u010Dn\u00ED \u010Dinidlo. Polo\u010Das rozpadu m\u016F\u017Ee v podzemn\u00EDch podm\u00EDnk\u00E1ch dosahovat a\u017E 100 let a TCP p\u0159etrv\u00E1v\u00E1 v prost\u0159ed\u00ED podzemn\u00EDch vod dlouhou dobu. TCP je velmi odoln\u00FD v\u016F\u010Di biodegradaci v d\u016Fsledku absence dehalogen\u00E1zy efektivn\u011B p\u0159em\u011B\u0148uj\u00EDc\u00ED TCP na dichlorpropanol. Proteinov\u00FDm in\u017Een\u00FDrstv\u00EDm a \u0159\u00EDzenou evoluc\u00ED jsme ned\u00E1vno zkonstruovali dehalogen\u00E1zu s v\u00FDznamn\u011B lep\u0161\u00ED katal\u00FDzou tohoto biochemick\u00E9ho kroku (Pavlov\u00E1 et al. 2009, Nat. Chem. Biol. 5: 727-733). C\u00EDlem tohoto projektu je sestavit kompletn\u00ED biochemickou dr\u00E1hu klonov\u00E1n\u00EDm gen\u016F enzym\u016F optimalizovan\u00FDch proteinov\u00FDm in\u017Een\u00FDrstv\u00EDm do p\u0159\u00EDrodn\u00EDch bakteri\u00ED, vedouc\u00ED ke konstrukci bakterie degraduj\u00EDc\u00ED TCP. Tato bakterie bude r\u016Fst na TCP jako jedin\u00E9m zdroji uhl\u00EDku a energie. Podle na\u0161ich informac\u00ED bude tato bakterie prvn\u00EDm mikroorganismem zkonstruovan\u00FDm proteinov\u00FDm a matabolick\u00FDm in\u017Een\u00FDrstv\u00EDm, pou\u017Eiteln\u00FDm k \u010Di\u0161t\u011Bn\u00ED vod kontaminovan\u00FDch antropogenn\u00ED slou\u010Deninou v uzav\u0159en\u00FDch pr\u016Fto\u010Dn\u00FDch syst\u00E9mech." . . . "2" . . "0"^^ . "2016-12-31+01:00"^^ . "Konstrukce syntetick\u00E9 metabolick\u00E9 dr\u00E1hy pro degradaci d\u016Fle\u017Eit\u00E9ho environment\u00E1ln\u00EDho polutantu proteinov\u00FDm a metabolick\u00FDm in\u017Een\u00FDrstv\u00EDm" . . "2014-03-19+01:00"^^ . . . "0"^^ . . . . . . "biodegradation environmental pollutant protein engineering synthetic biology 1" . . "http://www.isvav.cz/projectDetail.do?rowId=GAP503/12/0572"^^ . . . "Anthropogenic compound 1,2,3-trichloropropane (TCP) is released into the environment as a result of its manufacture and use as a solvent. Since its half-life under groundwater conditions is estimated to be up to 100 years, TCP is likely to persist for long period of time in the groundwater environment. TCP is very resistant to biodegradation, which can be attributed to a single biochemical step, lacking dehalogenase efficiently converting TCP to dichloropropanol. We have recently constructed dehalogenase with improved catalysis of this biochemical step (Pavlov\u00E1 et al. 2009, Nat. Chem. Biol. 5: 727-733). The objective of this project is to assemble entire biochemical pathway by cloning the genes optimized by protein engineering to native bacteria, leading to construction of TCP-degrading bacterium. This bacterium will grow on TCP as a sole carbon and energy source. To best of our knowledge, this bacterium will be the first example of a microorganism constructed by protein and matabolic engineering, applicable for the cleanup of contaminated water in the closed flow systems."@en . "biodegradation environmental pollutant protein engineering synthetic biology 1,2,3-trichloropropane"@en . "17"^^ . . "GAP503/12/0572" . "17"^^ . . .