"2010-01-01+01:00"^^ . . "2015-04-23+02:00"^^ . "Roles of the cysteine-rich and the sericin-type proteins in the silk fiber formation"@en . . . . . . "1"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GAP502/10/2382"^^ . . . "0"^^ . . "4"^^ . "Basic silk components fibroins and sericins are stored in the silk gland lumen as separate gels that are dehydrated and polymerize into solid materials during spinning. Centrally located fibroins condense rapidly into a filament, while sericins solidify with a delay. They seal the pair of filaments from the left and right glands into a single fiber and provide sticky fiber coating. Molecular interactions are partly known for some fibroin polymers but the mechanism of polymerization and the control of its rate are unknown for any silk protein. Proposed research targets two goals: (1) Verification of the posible role of proteins with cysteine-rich repeats in the silk filament polymerisation under water; (2) Identification of possible correlations between the primary structure, glycosylation, speed of polymerization, and the function of sericins. The conclusions based on the genomic and proteomic analyses of natural materials will be verified with the recombinant Cys-rich and sericin-like protein."@en . . "4"^^ . . "silk sericins proteinaceous polymers natural fibers recombinant silk proteins"@en . "0"^^ . "Z\u00E1kladn\u00ED slo\u017Eky hedv\u00E1b\u00ED fibroiny a sericiny jsou skladov\u00E1ny v dutin\u011B snovac\u00EDch \u017El\u00E1z jako odd\u011Blen\u00E9 gely, kter\u00E9 b\u011Bhem p\u0159eden\u00ED za ztr\u00E1ty vody polymeruj\u00ED v pevn\u00E9 materi\u00E1ly. St\u0159edov\u011B ulo\u017Een\u00E9 fibroiny rychle kondenzuj\u00ED ve filament, zat\u00EDmco sericiny, kter\u00E9 spojuj\u00ED p\u00E1r filament\u016F (z prav\u00E9 a lev\u00E9 \u017El\u00E1zy) v jedno vl\u00E1kno s lepiv\u00FDm povrchem, tuhnou se zpo\u017Ed\u011Bn\u00EDm. U n\u011Bkter\u00FDch fibroin\u016F jsou \u010D\u00E1ste\u010Dn\u011B zn\u00E1m\u00E9 interakce molekul v\u00A0polym\u00E9ru, av\u0161ak o \u0159\u00EDzen\u00ED rychlosti a mechanismu polymerizace nejsou \u017E\u00E1dn\u00E9 \u00FAdaje. Navrhovan\u00FD v\u00FDzkum je zam\u011B\u0159en na dva c\u00EDle: (1) Prov\u011B\u0159it, jakou roli hraj\u00ED p\u0159i\u00A0 polymerizaci hedv\u00E1b\u00ED pod vodou b\u00EDlkoviny s repeticemi bohat\u00FDmi na cystein;(2) Naj\u00EDt korelace mezi prim\u00E1rn\u00EDmi sekvencemi, glykosylac\u00ED, rychlost\u00ED polymerizace a funkc\u00ED sericin\u016F. Z\u00E1v\u011Bry genomick\u00E9ho a proteomick\u00E9ho v\u00FDzkumu p\u0159\u00EDrodn\u00EDch materi\u00E1l\u016F budou ov\u011B\u0159eny na rekombinantn\u00EDch b\u00EDlkovin\u00E1ch bohat\u00FDch na cystein nebo podobn\u00FDch sericin\u016Fm." . . . "2015-12-31+01:00"^^ . "GAP502/10/2382" . . . . . . "\u00DAloha protein\u016F bohat\u00FDch cysteinem a sericin\u016F v tvorb\u011B hedv\u00E1bn\u00E9ho vl\u00E1kna" . .