. . "Stres a signaling u rostlin pomoc\u00ED mitogen aktivovan\u00FDch proteinkinas: od z\u00E1kladn\u00EDho v\u00FDzkumu na Arabidopsis k biotechnologick\u00FDm aplikac\u00EDm na plodin\u00E1ch" . . . "2015-04-23+02:00"^^ . "Mitogeny aktivovan\u00E9 proteinkinasy (MAPKs) p\u0159edstavuj\u00ED kl\u00ED\u010Dov\u00E9 sign\u00E1ln\u00ED a regula\u010Dn\u00ED enzymy, kter\u00E9 se pod\u00EDl\u00ED na v\u00FDvoji rostlin a jejich reakci na abiotick\u00FD a biotick\u00FD stres. Hlavn\u00EDm c\u00EDlem tohoto projektu je studium MAPKs v pr\u016Fb\u011Bhu percepce a p\u0159enosu stresov\u00E9ho sign\u00E1lu na bun\u011B\u010Dn\u00E9 a org\u00E1nov\u00E9 \u00FArovni a \u00FArovni cel\u00E9ho oranismu u t\u0159\u00ED rostlinn\u00FDch druh\u016F Arabidopsis thaliana, Medicago sativa a Zea mays. V projektu budou pou\u017Eity genetick\u00E9 n\u00E1stroje jako nadexprese, gain of function, loss of function, uml\u010Dov\u00E1n\u00ED a deregulace gen\u016F se zam\u011B\u0159en\u00EDm na manipulaci a vylep\u0161en\u00ED obsahu a aktivity MAPKs p\u0159\u00EDmo v rostlin\u00E1ch. Nav\u00EDc budou aplikov\u00E1ny bun\u011B\u010Dn\u011B biologick\u00E9, biochemick\u00E9 a proteomick\u00E9 metody ke studiu mechanismu \u00FA\u010Dinku MAPKs b\u011Bhem stresu. Kombinovan\u00E9 funk\u010Dn\u011B genetick\u00E9, genomick\u00E9 a bun\u011B\u010Dn\u011B biologick\u00E9 p\u0159\u00EDstupy budou vyu\u017Eity na produkci nov\u00FDch dob\u0159e charakterizovan\u00FDch transgenn\u00EDch lini\u00ED rostlin s lep\u0161\u00ED toleranc\u00ED/rezistenci v\u016F\u010Di biotick\u00E9mu nebo abiotick\u00E9mu stresu. Tyto linie mohou b\u00FDt v bl\u00EDzk\u00E9 budoucnosti pou\u017Eity v biotechnologick\u00FDch a \u0161lechtitelsk\u00FDch programech." . "2015-12-31+01:00"^^ . . . "16"^^ . . "Mitogen-activated protein kinases (MAPKs) are key signalling and regulatory phosphorylation enzymes involved in plant development and reaction to abiotic and biotic stress. The main aim of this project is to study the role of MAPKs during stress perception, transduction and adaptation at cellular, organ and organismal levels in three model plant species Arabidopsis thaliana, Medicago sativa and Zea mays. Genetic tools (gene overexpression, gain of function, loss of function, silencing and down- or upregulation) will be developed to manipulate/improve MAPK content and activity in planta. Moreover, cell biological, biochemical and proteomic tools will be applied to study the mechanism of MAPKs involvement in plant stress. Combined functional genetic, genomic and cell biological approaches should provide new and well characterized transgenic lines of above mentioned plant species showing better tolerance/resistance against biotic and/or abiotic stress. These lines will be used in biotechnological and breeding programs in the near future."@en . . "2014-03-31+02:00"^^ . "16"^^ . . . "http://www.isvav.cz/projectDetail.do?rowId=GAP501/11/1764"^^ . "0"^^ . "Mitogen-activated protein kinase stress signalling in plants: from basic research in Arabidopsis towards biotechnological applications in crops."@en . "stress tolerance resistance plant signalling mitogen-activated protein kinase cytoskeleton"@en . "GAP501/11/1764" . . . "1"^^ . . . "2011-01-01+01:00"^^ . . . "0"^^ . . . .