. . . "0"^^ . . "Mitochondrie nejsou sou\u010D\u00E1st\u00ED sekre\u010Dn\u00ED dr\u00E1hy a jako takov\u00E9 p\u0159\u00EDj\u00EDmaj\u00ED proteiny pomoc\u00ED specifick\u00FDch membr\u00E1nov\u00FDch translok\u00E1z. Velikost mitochondri\u00E1ln\u00ED proteom\u016F se dramaticky m\u011Bn\u00ED nap\u0159\u00ED\u010D jednotliv\u00FDmi druhy eukaryot. V p\u0159\u00EDrod\u011B tak nalezneme sav\u010D\u00ED mitochondrie s v\u00EDce jak t\u00EDs\u00EDcem r\u016Fzn\u00FDch protein\u016F, ale i mitochondrie prvok\u016F, kter\u00E9 obsahuj\u00ED jen n\u011Bkolik enzym\u016F. My se zab\u00FDv\u00E1me v\u00FDzkumem biogeneze t\u011Bchto velice redukovan\u00FDch organel, kter\u00E9 ztratily sv\u00E9 mitochondrialn\u00ED genomy a n\u011Bkter\u00E9 dokonce i metabolick\u00E9 dr\u00E1hy pro tvorbu ATP. P\u0159esto jsou v membr\u00E1n\u00E1ch t\u011Bchto organel p\u0159\u00EDtomny zakladn\u00ED stavebn\u00ED kameny mitochondri\u00E1ln\u00EDch translok\u00E1z. Studiem t\u011Bchto minimalistick\u00FDch transportn\u00EDch komplex\u016F jsme objevili z\u00E1sadn\u00ED funk\u010Dn\u00ED rozd\u00EDly, kter\u00E9 n\u00E1m mohou pomoci objasnit obecn\u00E9 procesy prob\u00EDhaj\u00EDc\u00ED u v\u0161ech mitochondri\u00ED. Specifick\u00E9 c\u00EDle tohoto projektu jsou (i) objasn\u011Bn\u00ED importu a uspo\u0159\u00E1d\u00E1n\u00ED multimern\u00EDch mitochondri\u00E1ln\u00EDch chaperon\u016F v mezimembr\u00E1novem prostoru (ii) objasn\u011Bn\u00ED transportu protein\u016F, kter\u00FD je nez\u00E1visl\u00FD na kl\u00ED\u010Dov\u00E9 mitochondrialn\u00ED TIM translok\u00E1ze a (iii) zp\u016Fsob integrace mitochondri\u00ED do sekre\u010Dn\u00ED dr\u00E1hy." . "Mitochondria stand outside the secretory pathway and as such they contain a set of molecular machines in their membranes, by which they transport proteins translated on the cytosolic ribosomes. Across the eukaryotic diversity mitochondrial proteoms can range from over thousand proteins in mammalian cells to just a handful in some unicellular eukaryotes. We study the biogenesis of the simplest mitochondria found yet. While these organelles lost their own genome and even, in some cases, the ATP generation pathway, they still use the core components of the molecular machines transporting mitochondrial proteins. By studying these minimalist complexes we have found unprecedented functional differences in these simple mitochondria, which can help us to understand molecular processes occurring in all mitochondria. Particularly, in this project we will examine (i) the protein import and assembly of mitochondrial small Tim chaperones, (ii) the functional replacement of key mitochondrial TIM translocase and (iii) and integration of mitochondria into secretory pathway."@en . . "2014-12-31+01:00"^^ . "1"^^ . . . "mitochondria protein import mitosome hydrogenosome vesicular transport"@en . "V r\u00E1mci projektu se n\u00E1m poda\u0159ilo publikovat celkem 6 publikac\u00ED v impaktovan\u00FDch \u010Dasopisech. Z\u00EDskan\u00E9 v\u00FDsledky v\u00FDznamn\u011B posunuly na\u0161e v\u011Bdomosti o biogenezi redukovan\u00FDch mitochondri\u00ED a o celkov\u00E9 evoluci n\u011Bkter\u00FDch aspekt\u016F mitochondri\u00E1ln\u00EDho importu protein\u016F."@cs . . "The evolution of mitochondrial biogenesis."@en . "0"^^ . "2015-05-22+02:00"^^ . . "During the project six publications with the grant dedication were published in the impacted journals. The overall results greatly enriched our understanding not only of the biogenesis of the reduced mitochondria but also of the evolution of several general aspects of the mitochondrial protein import."@en . . "2014-03-31+02:00"^^ . "5"^^ . . "2010-01-01+01:00"^^ . "5"^^ . "Evoluce mitochondri\u00E1ln\u00ED biogeneze." . . . "GAP305/10/0651" . . . . . . "http://www.isvav.cz/projectDetail.do?rowId=GAP305/10/0651"^^ .