"GAP305/10/0433" . . . . . . . . "formins FH2 proteins actin evolution cell morphogenesis"@en . "Characterization of selected representatives of novel plant Class II and Class III formin families"@en . "2010-01-01+01:00"^^ . "2014-07-01+02:00"^^ . "2013-06-12+02:00"^^ . . "Formins, or FH2 proteins, are an evolutionarily conserved family of actin-organizing proteins exhibiting a great diversity of domain combinations across eukaryotes. Only a small fraction of this variety has been experimentally studied so far. Plants have three groups of formins. For two of them - Class II (where we previously discovered a novel domain related to the PTEN antioncogene), and Class III (recently described by us, found only in lower plants and characterized by the presence of a RhoGAP-like domain) -\u00A0 no experimental report has been published yet. We plan to characterize selected representatives of Class II and Class III formins from Arabidopsis thaliana and Physcomitrella patens, respectively, with respect to their cellular localization, protein-protein interactions, and biological function. The results should contribute to the understanding of the mechanisms governing plant cytoskeletal organization, as well as to grasping the more general problem how combination of conserved domains contributes to the vast diversity of form and function found in eukaryotes."@en . . "7"^^ . . "7"^^ . "The project lead to substantial insights into relationships between plant formins and dynamics of the actin cytoskeleton. The results were published in 3 original papers, 3 reviews and 1 methodological paper, project can thus be evaluated as accomplished. One master and 1 PhD theses were defended. No shortcomings regarding adherence to grant administration rules were found."@en . "2013-12-31+01:00"^^ . . "1"^^ . "1"^^ . . . . . "Forminy (FH2 proteiny) jsou skupina evolu\u010Dn\u011B konzervovan\u00FDch aktin-organizuj\u00EDc\u00EDch protein\u016F, kter\u00E9 se vyzna\u010Duj\u00ED velikou rozmanitost\u00ED dom\u00E9nov\u00E9 stavby u r\u016Fzn\u00FDch eukaryot. Jen mal\u00E1 \u010D\u00E1st t\u00E9to diversity byla dosud uchopena experiment\u00E1ln\u011B. Rostliny maj\u00ED t\u0159i skupiny formin\u016F. Pro dv\u011B z nich - t\u0159\u00EDdu II (v n\u00ED\u017E jsme d\u0159\u00EDve popsali novou dom\u00E9nu p\u0159\u00EDbuznou antionkogenu PTEN) a t\u0159\u00EDdu III (kterou jsme ned\u00E1vno objevili u ni\u017E\u0161\u00EDch rostlin a pro ni\u017E je typick\u00E1 dom\u00E9na p\u0159\u00EDbuzn\u00E1 RhoGAP) - dosud nebyla zve\u0159ejn\u011Bna \u017E\u00E1dn\u00E1 experiment\u00E1ln\u00ED data.Na\u0161\u00EDm c\u00EDlem je charakterizovat vybran\u00E9 z\u00E1stupce formin\u016F t\u0159\u00EDdy II a III z modelov\u00FDch rostlin Arabidopsis thaliana a Physcomitrella patens s ohledem na bun\u011B\u010Dnou lokalizaci, protein-proteinov\u00E9 interakce a biologickou funkci.V\u00FDsledky p\u0159isp\u011Bj\u00ED k pochopen\u00ED mechanism\u016F \u0159\u00EDd\u00EDc\u00EDch uspo\u0159\u00E1d\u00E1n\u00ED rostlinn\u00E9ho cytoskeletu i k pochopen\u00ED obecn\u011Bj\u0161\u00ED ot\u00E1zky, jak m\u016F\u017Ee kombinace evolu\u010Dn\u011B star\u00FDch dom\u00E9n p\u0159isp\u00EDvat k rozmanitosti struktur i funkc\u00ED eukaryotn\u00EDch bun\u011Bk." . "http://www.isvav.cz/projectDetail.do?rowId=GAP305/10/0433"^^ . . "0"^^ . . . . "Charakterizace vybran\u00FDch p\u0159\u00EDslu\u0161n\u00EDk\u016F nov\u00FDch skupin rostlinn\u00FDch formin\u016F - t\u0159\u00EDdy II a t\u0159\u00EDdy III." . . "\u00DAsp\u011B\u0161n\u00E9 \u0159e\u0161en\u00ED grantu vedlo k z\u00EDsk\u00E1n\u00ED v\u00FDznamn\u00FDch poznatk\u016F o souvislostech mezi rostlinn\u00FDmi forminy a dynamikou aktinov\u00E9ho cytoskeletu. V\u00FDsledky byly publikov\u00E1ny v 3 p\u016Fvodn\u00EDch a 3 p\u0159ehledn\u00FDch sd\u011Blen\u00EDch, d\u00E1le v metodick\u00E9 pr\u00E1ci, projekt lze tedy hodnotit jako spln\u011Bn\u00FD. Byla obh\u00E1jena diplomov\u00E1 a diserta\u010Dn\u00ED pr\u00E1ce. Nebyly shled\u00E1ny nedostatky stran dodr\u017Eov\u00E1n\u00ED grantov\u00FDch pravidel."@cs .