"Glycin je hlavn\u00ED inhibi\u010Dn\u00ED p\u0159ena\u0161e\u010D m\u00EDchy a mozkov\u00E9ho kmene. Ve sluchov\u00E9 dr\u00E1ze se glycinergn\u00ED neurony nach\u00E1z\u00ED p\u0159edev\u0161\u00EDm v syst\u00E9mu olivy superior complex (SOC). Endogenn\u00ED zdroje glycinu v SOC dosud nebyly pln\u011B objasn\u011Bny. Navrhujeme studovat mechanismy ektopick\u00E9ho uvol\u0148ov\u00E1n\u00ED glycinu v medi\u00E1ln\u00EDm j\u00E1d\u0159e trap\u00E9zovit\u00E9ho t\u011Blesa (MNTB) potkana pomoc\u00ED elektrofyziologick\u00FDch, imunohistochemick\u00FDch a molekul\u00E1rn\u011B biologick\u00FDch metod. Budeme studovat somatodendritick\u00E9 uvoln\u011Bn\u00ED glycinu zprost\u0159edkovan\u00E9 reverzn\u00ED \u010Dinnost\u00ED glycinov\u00FDch p\u0159ena\u0161e\u010D\u016F a Ca2+-z\u00E1visl\u00FDm zp\u016Fsobem. D\u00E1le budeme zkoumat fyziologick\u00FD v\u00FDznam somatodendritick\u00E9ho uvoln\u011Bn\u00ED a zjist\u00EDme podm\u00EDnky za nich\u017E je vyvol\u00E1v\u00E1n. Na\u0161e pozornost bude soust\u0159ed\u011Bna na retrogr\u00E1dn\u00ED modulaci glutamatergn\u00EDho synaptick\u00E9ho p\u0159enosu prost\u0159ednictv\u00EDm glycinu poch\u00E1zej\u00EDc\u00EDho z postsynaptick\u00E9 bu\u0148ky. Ur\u010D\u00EDme tak\u00E9 distribuci glycinov\u00FDch p\u0159ena\u0161e\u010D\u016F v bu\u0148k\u00E1ch MNTB pomoc\u00ED konfok\u00E1ln\u00ED a imunoelektronov\u00E9 mikroskopie. V\u00FDsledky na\u0161eho projektu pomohou pochopit mechanismy glycinergn\u00EDho p\u0159enosu a glycinem-zprost\u0159edkovan\u00E9 modulace excita\u010Dn\u00EDho p\u0159enosu na synaps\u00EDch centr\u00E1ln\u00EDho typu." . . . "1"^^ . "GAP303/11/0131" . . "3"^^ . "2015-12-31+01:00"^^ . . . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GAP303/11/0131"^^ . . "3"^^ . . . "Fyziologick\u00FD v\u00FDznam a mechanismus ektopick\u00E9ho uvol\u0148ov\u00E1n\u00ED glycinu" . . "2011-01-01+01:00"^^ . "Ectopic release of glycine - physiological role and mechanism"@en . . . . "synaptic transmission glycine transport"@en . . . . . "Glycineis the major inhibitory transmitter in the spinal cord and the brainstem. In the auditory pathway, glycinergic neurons are prominent in the superior olivary complex (SOC). The endogenous sources of glycine in the SOC were not fully elucidated yet. We propose to study mechanisms of glycine ectopic release inthe rat medial nucleus of trapezoid body (MNTB) using electrophysiology,immunohistochemistry and molecular biology methods. We will study the somatodendritic release of glycine (SDR) mediated by reverse action of glycine transporters and by a Ca-dependent mechanism. We will also investigate the physiological relevance of SDR and the conditions under which it is evoked.An attention will be paid to the modulation of glutamatergic synaptic transmission by glycine originating from SDR. We will determine the subcellular distribution and trafficking of glycine transporters in the MNTB by confocal and immunoelectron microscopy. Our study will help to understand the mechanisms of glycine-mediated signaling and modulation of excitatory transmission at central type synapses."@en . "0"^^ . "2015-04-23+02:00"^^ . . .