. "5"^^ . "cytoskeleton microtubules gamma-tubulin,cell cycle cancer cells"@en . "\u00DAloha gama-tubulinov\u00FDch komplex\u016F p\u0159i regulaci bun\u011B\u010Dn\u00E9ho cyklu" . "5"^^ . . "2014-03-31+02:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GAP302/12/1673"^^ . . "γ-Tubulin is the key player in microtubule nucleation. While abnormalities in microtubule organization constitute a common pattern of genetic instability in cancer cells, the molecular mechanisms underlying such changes are unknown. Previously we have described overexpression of γ-tubulin in malignant tumors. Newly we identified its association with C53 tumor suppressor that acts as a regulator of G2/M progression, and with the K0776 protein that regulates C53 functions. The aim of this research project is to clarify the role of novel γ-tubulin complexes in cell cycle progression. To this end we propose to analyze the localization of these complexes in living cells together with their biochemical properties and function(s) during cell cycle progression in normal and transformed cells. We will use qRT-PCR, antibodies, tagged versions of proteins and RNAi techniques in combination with immunofluorescence microscopy and live-cell imaging. These studies should provide better understanding of γ-tubulin function(s) in the regulation of cell cycle progression."@en . . "Role of gamma-tubulin complexes in regulation of cell cycle progression"@en . . "0"^^ . . "2012-01-01+01:00"^^ . . . . "0"^^ . "GAP302/12/1673" . "2015-04-23+02:00"^^ . . . "1"^^ . . . . . "cytoskeleton microtubules gamma-tubulin" . . . "γ-Tubulin je nezbytn\u00FD pro nukleaci a organizaci mikrotubul\u016F. Zat\u00EDmco naru\u0161en\u00ED organizace mikrotubul\u016F p\u0159edstavuje zn\u00E1m\u00FD mechanismus genetick\u00E9 nestability n\u00E1dorov\u00FDch bun\u011Bk, molekul\u00E1rn\u00ED podstata tohoto jevu je nezn\u00E1m\u00E1. D\u0159\u00EDve jsme popsali zv\u00FD\u0161enou expresi γ-tubulinu u malign\u00EDch n\u00E1dor\u016F. Nov\u011B jsme identifikovali asociaci γ-tubulinu jak s proteinem C53, kter\u00FD p\u0159edstavuje n\u00E1dorov\u00FD supresor a reguluje v bu\u0148k\u00E1ch G2/M p\u0159echod, tak i s proteinem K0776, jen\u017E je regul\u00E1torem C53. C\u00EDlem thoto projektu je pochopit \u00FAlohu nov\u00FDch γ-tubulinov\u00FD komplex\u016F p\u0159i regulaci bun\u011B\u010Dn\u00E9ho cyklu. Navrhujeme analyzovat jak lokalizaci komplex\u016F v \u017Eiv\u00FDch bu\u0148k\u00E1ch, tak i jejich biochemick\u00E9 vlastnosti a funkce v pr\u016Fb\u011Bhu bun\u011B\u010Dn\u00E9ho cyklu. Zm\u011Bny v nukleaci a dynamice mikrotubul\u016F budou sledov\u00E1ny u bun\u011Bk se sn\u00ED\u017Eenou expres\u00ED K0776 a C53. Pro tato studia pou\u017Eijeme qRT-PCR, specifick\u00E9 protil\u00E1tky, zna\u010Den\u00E9 a mutovan\u00E9 verze protein\u016F a RNAi techniky v kombinci s pokro\u010Dil\u00FDmi mikroskopick\u00FDmi technikami. Uveden\u00E1 studia p\u0159inesou nov\u00E9 poznatky o \u00FAloze γ-tubulin p\u0159i regulaci bun\u011B\u010Dn\u00E9ho cyklu." . . "2015-12-31+01:00"^^ .