"7"^^ . "7"^^ . "Hlavn\u00EDm c\u00EDlem tohoto projektu je porozum\u011Bn\u00ED strukturn\u00ED podstaty regulace aktivity enzymu neutr\u00E1ln\u00ED trehalasy (NTH1) prost\u0159ednictv\u00EDm vazby BMH protein\u016F. Hlavn\u00EDmi metodami budou c\u00EDlen\u00E1 mutageneze, stacion\u00E1rn\u00ED a \u010Dasov\u011B rozli\u0161en\u00E1 fluorescen\u010Dn\u00ED spektroskopie, proteinov\u00E1 krystalografie, analytick\u00E1 ultracentrifugace a studium enzymov\u00E9 kinetiky. NTH1 je zodpov\u011Bdn\u00E1 za degradaci trehalosy a je vy\u017Eadov\u00E1na v \u0159ad\u011B stresov\u00FDch situac\u00ED. Ned\u00E1vno bylo zji\u0161t\u011Bno, \u017Ee aktivita enzymu NTH1 je v kvasink\u00E1ch regulov\u00E1na vazbou BMH protein\u016F. \u00DAloha kvasni\u010Dn\u00FDch BMH protein\u016F v regulaci NTH1 se zd\u00E1 b\u00FDt zvy\u0161ov\u00E1n\u00ED enzymov\u00E9 aktivity NTH1 enzymu. Av\u0161ak detaily t\u00FDkaj\u00EDc\u00ED se interakce mezi BMH proteiny a NTH1 st\u00E1le z\u016Fst\u00E1vaj\u00ED neobjasn\u011Bny. Pon\u011Bvad\u017E metabolismus trehalosy je z\u00E1kladn\u00ED slo\u017Ekou odpov\u011Bdi na stres v bu\u0148k\u00E1ch kvasinek, objasn\u011Bn\u00ED mechanism\u016F regulace aktivity NTH1, kter\u00E9 je st\u00E1le nevy\u0159e\u0161eno, n\u00E1m poskytne velmi d\u016Fle\u017Eitou informaci t\u00FDkaj\u00EDc\u00ED se mechanismu jak funkce 14-3-3 proteinu, tak i regulace aktivity neutr\u00E1ln\u00ED trehalasy v kvasink\u00E1ch." . . . . . . . . . "2011-01-01+01:00"^^ . . . "2014-03-31+02:00"^^ . "Role of BMH proteins in the regulation of yeast enzyme neutral trehalase"@en . "The overall goal of this project is tounderstand the structural basis of BMH\u2013dependent regulation of the activity ofthe enzyme Neutral trehalase (NTH1). Site-directed mutagenesis, steady-statefluorescence spectroscopy, time-resolved fluorescence spectroscopy, X-raycrystallography, analytical ultracentrifugation and enzyme kineticsmeasurements will be the principal tools. NTH1 is responsible for trehalosedegradation and is required in a variety stress conditions. Activity of theNTH1 enzyme was just recently found to be mediated by BMH1 and BMH2 binding inyeast. The role of yeast BMH proteins in the regulation of NTH1 seems toincreasethe enzymatic activity of the NTH1 enzyme. However details concerninginteraction between the BMH proteins and NTH1 remain still unknown. Sincetrehalose metabolism is an essential component of the stress response inyeastcells, thus, the elucidation of the mechanisms of NTH1 protein regulation,which is still unresolved, would provide us with important informationconcerning mechanisms of both the 14-3-3 protein function and the regulation ofthe neutral trehalase activity in yeast."@en . "0"^^ . "14-3-3 protein neutral trehalase fluorescence activity assay protein crystallography"@en . . . "http://www.isvav.cz/projectDetail.do?rowId=GAP207/11/0455"^^ . "2015-04-23+02:00"^^ . . . "0"^^ . "2015-12-31+01:00"^^ . . . "1"^^ . "\u00DAloha BMH protein\u016F v regulaci kvasni\u010Dn\u00E9ho enzymu neutr\u00E1ln\u00ED trehal\u00E1sy" . . "GAP207/11/0455" . .