"0"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=GAP207/10/1040"^^ . . "0"^^ . . "\u0158e\u0161en\u00ED projektu odpov\u00EDdalo z\u00E1m\u011Br\u016Fm. V\u00FDsledkem je p\u011Bt publikac\u00ED. Projekt byl ale po t\u0159ech letech \u0159e\u0161en\u00ED panelem p\u0159ed\u010Dasn\u011B ukon\u010Den. D\u016Fvodem byla \u00FA\u010Dast Prof. Bezou\u0161ky usv\u011Bd\u010Den\u00E9ho ze \u201Escientific misconduct\u201C a jeho pod\u00EDl na publikac\u00EDch vze\u0161l\u00FDch z projektu a zab\u00FDvaj\u00EDc\u00EDch se NKR receptory, kter\u00FDch se neetick\u00E9 po\u010D\u00EDn\u00E1n\u00ED Prof. Bezou\u0161ky t\u00FDkalo. Proto je mo\u017En\u00E9 ohodnotit projekt pouze jako spln\u011Bn\u00FD s v\u00FDhradou."@cs . . . . "Strukturn\u00ED anal\u00FDza proteinov\u00FDch komplex\u016F p\u0159irozen\u00FDch zab\u00EDje\u010D\u016F pomoc\u00ED chemick\u00E9ho zes\u00EDt\u011Bn\u00ED, isotopov\u00E9 v\u00FDm\u011Bny, hmotnostn\u00ED spektrometrie a NMR." . . . "The grant project followed the aims and resulted in five publications. After three years, the project was aborted by the committee by reason of the participation of Prof. Bezou\u0161ka who was found guilty of a scientific misconduct due to the unethical manipulations with NKR receptors and who co-authorized four project publications. The final evaluation of the project is \u201Cfulfilled with objections\u201D."@en . "Natural killer cells do not express at their surface a single dominant activation receptor similar to the T or B cell receptor. Therefore, the immune effector functions of these cells are regulated by a large number of receptors conferring either inhibitory or activation signals as a part of the large receptor zipper. In order to understand the mechanisms details beyond the recognition of tumor or virally infected cells by natural kille cells, it is essential to study the molecular details of receptor \u2013 ligand interactions using highly purified soluble\u00A0 receptors. In the present project we suggest to produce at least four highly soluble recombinant receptors of natural killer cells and their potential ligands, namely mNKR-P1A, mNKR-P1C, mNKR-P1F, and mClr-g, and to study their interactions and the structure of the receptor \u2013 ligand complexes using chemical cross-linking, H \u2013 D exchange, mass spectrometry, and protein NMR."@en . . "Analysis of protein complexes of killer cell receptors using chemical cross-linking, surface labeling, isotope exchange, mass spectrometry and NMR."@en . "GAP207/10/1040" . "2010-01-01+01:00"^^ . . . "protein complexes chemical cross-linking isotope exchange mass spectrometry nuclear magnetic resonance molecular dynamic"@en . . "P\u0159irozen\u00E9 zab\u00EDje\u010Dsk\u00E9 bu\u0148ky neexprimuj\u00ED na sv\u00E9m povrchu jedin\u00FD dominantn\u00ED aktiva\u010Dn\u00ED receptor, ale jejich efektorov\u00E9 funkce jsou regulov\u00E1ny celou \u0159adou receptor - ligandov\u00FDch interakc\u00ED tvo\u0159\u00EDc\u00EDch tzv. receptorov\u00FD zip. Pro lep\u0161\u00ED pochopen\u00ED molekul\u00E1rn\u00EDch mechanism\u016F rozpozn\u00E1n\u00ED n\u00E1dorov\u00FDch a virov\u011B infikovan\u00FDch bun\u011Bk p\u0159irozen\u00FDmi zab\u00EDje\u010Dsk\u00FDmi bu\u0148kami je nezbytn\u00E9 studovat tyto interakce receptor\u016F s c\u00EDlov\u00FDmi ligandy jednotliv\u011B s pou\u017Eit\u00EDm vysoce purifikovan\u00FDch receptor\u016F. V p\u0159edkl\u00E1dan\u00E9m projektu navrhujeme produkovat nejm\u00E9n\u011B 4 vysoce rozpustn\u00E9 receptory p\u0159irozen\u00FDch zab\u00EDje\u010Dsk\u00FDch bun\u011Bk a jejich ligandy, a pot\u00E9 studovat komplexy receptor - ligand pomoc\u00ED chemick\u00FDch s\u00ED\u0165ovac\u00EDch \u010Dinidel, isotopov\u00E9 v\u00FDm\u011Bny, hmotnostn\u00ED spektrometrie a proteinov\u00E9 NMR." . . . "2014-07-01+02:00"^^ . "9"^^ . . . "9"^^ . . "Na \u017E\u00E1dost \u017Eadatele nebo GA\u010CR (nen\u00ED v syst\u00E9mu GA\u010CR evidov\u00E1no)"@cs . . . "2013-02-28+01:00"^^ . . "At the request of the applicant or GACR (not registered in the GACR system)"@en . "1"^^ .