"N-Acetyl-D-mannosamin (ManNAc) a n\u011Bkter\u00E9 sacharidy obsahuj\u00EDc\u00ED ManNAc (nap\u0159., GlcNAc-\u03B2-(1\u21924)ManNAc) maj\u00ED siln\u00E9 imunomodula\u010Dn\u00ED vlastnosti. Chemick\u00E1 synt\u00E9za takov\u00FDchto slou\u010Denin je velice komplikovan\u00E1. Enzymov\u00E1 hydrol\u00FDza ani transfer N-acetyl-D-mannosaminid\u016F nebyl dosud v\u00A0literatu\u0159e pops\u00E1n. Tento projekt se zab\u00FDv\u00E1 jak hled\u00E1n\u00EDm \u03B2-N-acetylmannosidasy (mikrobi\u00E1ln\u00EDho, rostlinn\u00E9ho i \u017Eivo\u010Di\u0161n\u00E9ho p\u016Fvodu) -tak p\u0159\u00EDpravou mutantn\u00ED \u03B2-mannosidasy se schopnost\u00ED \u0161t\u011Bpit a pop\u0159\u00EDpad\u011B i syntetizovat sacharidy obsahuj\u00EDc\u00ED \u03B2-ManNAc na neredukuj\u00EDc\u00EDm konci cukern\u00E9ho \u0159et\u011Bzce." . "2013-06-12+02:00"^^ . "N-acetyl-D-mannosamine beta-mannosidase"@en . "The search for \u03B2-N-acetylmannosidase cleaving/synthesizing \u03B2-ManNAc-saccharides was not successful. However, the authors prepared \u03B2-mannosidase, including its mutants and crystal structure. The results are important for the field. The authors published 7 good quality papers and one chapter. Management of the funds was correct. The project can be evaluated as fulfilled."@en . . "2013-12-31+01:00"^^ . "Enzymy metabolismu mannosid\u016F v p\u0159\u00EDprav\u011B N-acetyl-mannosaminov\u00FDch struktur" . "0"^^ . . . . . "GAP207/10/0321" . "2010-01-01+01:00"^^ . . "The ways to N-acetyl-mannosamine structures via mannoside metabolizing enzymes"@en . . "1"^^ . "1"^^ . . "Projekt se zab\u00FDval hled\u00E1n\u00EDm \u03B2-N-acetylmannosidasy \u0161t\u011Bp\u00EDc\u00ED/syntetizuj\u00EDc\u00ED sacharidy obsahuj\u00EDc\u00ED \u03B2-ManNAc co\u017E se p\u0159es ve\u0161ker\u00E9 \u00FAsil\u00ED nepoda\u0159ilo. Nicm\u00E9n\u011B byla p\u0159ipravena \u03B2-mannosidasa v\u010Detn\u011B mutant\u016F a krystalov\u00E9 struktury. V\u00FDsledky jsou pro obor d\u016Fle\u017Eit\u00E9. Auto\u0159i publikovali kapitolu v knize a 7 \u010Dl\u00E1nk\u016F v kvalitn\u00EDch \u010Dasopisech. Hospoda\u0159en\u00ED bylo v po\u0159\u00E1dku. Projekt je mo\u017En\u00E9 pova\u017Eovat za spln\u011Bn\u00FD."@cs . "2014-07-01+02:00"^^ . . . . . . "N-Acetyl-D-mannosamine (ManNAc), and some ManNAc-containing saccharides (e.g., GlcNAc-\u03B2-(1-4)ManNAc) were found to be strong immunostimulants. A series of chemical syntheses of ManNAc-containing compounds were recently published, but these syntheses are very complicated. To the best of our knowledge, \u03B2-N-acetylmannosidases or \u03B2-N-acetylmannosyltransferases, which could catalyze the cleavages/enzymatic synthesis of \u03B2-ManNAc-containing structures, have not yet been found. This project is focused on searching for enzymes with \u03B2-N-acetylmannosidase activities, and in parallel on the construction of a mutant \u03B2-mannosidase with altered biocatalytic abilities, aiming for \u03B2-N-acetylmannosidase activity."@en . "17"^^ . . . . "17"^^ . . . "http://www.isvav.cz/projectDetail.do?rowId=GAP207/10/0321"^^ .