. . . "Induced organization of biomolecules at interfaces by interacting with electrically charged surfaces as a tool for medical diagnostics"@en . . . . . "0"^^ . . . "Byly z\u00EDsk\u00E1ny nov\u00E9 poznatky o struktu\u0159e a uspo\u0159\u00E1d\u00E1n\u00ED nukleov\u00FDch kyselin a oligodeoxynukleotid\u016F na rozhran\u00EDch s elektricky nabit\u00FDmi povrchy. Byly p\u0159ipraveny a charakterizov\u00E1ny nanostrukturovan\u00E9 povrchy, jejich\u017E pou\u017Eit\u00ED umo\u017Enilo zlep\u0161it citlivost a selektivitu n\u011Bkolika typ\u016F biomolekul v\u00FDznamn\u00FDch pro v\u010Dasnou detekci chorob. Bylo publikov\u00E1no 14 prac\u00ED (8 Jimp) v mezin\u00E1rodn\u00EDch \u010Dasopisech a sborn\u00EDc\u00EDch."@cs . . "0"^^ . . "The aim of this project is extended and generalized the knowledge about the structure and organization of nucleic acids (DNA) and model synthetic deoxynucleotides (ODNs) at interfaces induced by interaction of these biomolecules with electrically charged surfaces. We will focus on study of (i) self-assembling of ODNs at electrically charged surfaces by two-dimensional condensation, (ii) conformation changes in the structure of DNA and model ODNs at electrically charged interfaces compared to their structures in bulk solution with respect to their nucleotide sequence and secondary structure, and (iii) relation between the surface treatment of electrode materials and the behavior of biomolecules at these surfaces. The new biophysical methods will be utilized in development of novel DNA sensor and medical sensor monitoring natural metabolites and pharmaceuticals in human blood or urine. We will study the interfacial behavior of blood plasma proteins on titanium surfaces coated by diamond-like carbon layers with the aim to find the optimal conditions of osseointegration of the implanted devices."@en . "1"^^ . "C\u00EDlem projektu je roz\u0161\u00ED\u0159it a zobecnit v\u011Bdomosti o struktu\u0159e a uspo\u0159\u00E1d\u00E1n\u00ED nukleov\u00FDch kyselin (DNA) a modelov\u00FDch syntetick\u00FDch oligodeoxynukleotid\u016F (ODNs) na f\u00E1zov\u00FDch rozhran\u00EDch vyvolan\u00FDch interakcemi t\u011Bchto biomakromolekul s\u00A0elektricky nabit\u00FDmi povrchy. Zam\u011B\u0159\u00EDme se na studium (i) tvorby samouspo\u0159\u00E1dan\u00FDch ODN monovrstev procesem dvoj-dimenzion\u00E1ln\u00ED kondenzace, (ii) konforma\u010Dn\u00EDch zm\u011Bn struktur DNA a modelov\u00FDch ODNs prob\u00EDhaj\u00EDc\u00EDch na elektricky nabit\u00FDch povr\u0161\u00EDch v\u00A0z\u00E1vislosti na jejich nukleotidov\u00E9 sekvenci a sekund\u00E1rn\u00ED struktu\u0159e v\u00A0porovn\u00E1n\u00ED se strukturami t\u011Bchto molekul ve vodn\u00E9m prost\u0159ed\u00ED, a (iii) vztahu mezi chov\u00E1n\u00EDm biomakromolekul na f\u00E1zov\u00FDch rozhran\u00EDch a povrchovou \u00FApravou elektrod. Budou zavedeny nov\u00E9 biofyzik\u00E1ln\u00ED metody umo\u017E\u0148uj\u00EDc\u00ED v\u00FDvoj nov\u00FDch DNA senzor\u016F a senzor\u016F pro l\u00E9ka\u0159stv\u00ED monitoruj\u00EDc\u00ED p\u0159irozen\u00E9 metabolity a l\u00E9\u010Diva v\u00A0krvi a mo\u010Di. Budeme studovat chov\u00E1n\u00ED protein\u016F lidsk\u00E9 plazmy na titanov\u00FDch povr\u0161\u00EDch s\u00A0napa\u0159en\u00FDmi DLC vrstvami s\u00A0c\u00EDlem naleznout optim\u00E1ln\u00ED podm\u00EDnky pro bezprobl\u00E9mov\u00E9 vhojen\u00ED aplikovan\u00FDch implant\u00E1t\u016F." . . "New results about the structure and organization of nucleic acids and oligodeoxynucleotides at interfaces with electrically charged surfaces were obtained. Nanostructured surfaces with improved sensitivity and selectivity for biomolecules, which are important for early disease detection, were prepared and characterized. 14 papers (8 Jimp) were published in international journals."@en . "Organizov\u00E1n\u00ED biomolekul na f\u00E1zov\u00FDch rozhran\u00EDch vyvolan\u00E9 jejich interakcemi s elektricky nabit\u00FDmi povrchy jako n\u00E1stroj pro l\u00E9ka\u0159skou diagnostiku" . "2015-05-22+02:00"^^ . . "http://www.isvav.cz/projectDetail.do?rowId=GAP205/10/2378"^^ . . "2014-03-31+02:00"^^ . . "6"^^ . "2010-01-01+01:00"^^ . . "GAP205/10/2378" . "2014-12-31+01:00"^^ . . "6"^^ . . . . "oligodeoxynucleotides DNA adsorption self-assembling of DNA DNA hybridization DNA interaction with electrically charged surfaces adsorption of proteins detection of metabolites and pharmaceuticals in urine and blood nanomaterials surface structure"@en .