" (nrDNA)" . " nuclear" . "2012-03-30+02:00"^^ . "Glomus; community; inoculation; nuclear; ribosomal; DNA; (nrDNA); mitochondrial; DNA; (mtDNA); real-time; PCR"@en . . " real-time" . . "Projekt p\u0159isp\u011Bl k porozum\u011Bn\u00ED ekologie mykorrhizn\u00EDch rostlin a na jeho v\u00FDstupy m\u016F\u017Ee nav\u00E1zat aplikovan\u00FD v\u00FDzkum. V\u00FDsledky byly publikov\u00E1ny v jedn\u00E9 velmi kvalitn\u00ED (Applied and Environmental Microbiology IF 3.8) a jedn\u00E9 kvalitn\u00ED publikaci, z nich\u017E prvn\u00ED je dedikov\u00E1na pouze projektu; dal\u0161\u00ED dva manuskripty jsou p\u0159ilo\u017Eeny. Projekt p\u0159isp\u011Bl k v\u00FDchov\u011B student\u016F, nedostatky v \u010Derp\u00E1n\u00ED financ\u00ED nebyly."@cs . "Glomus" . . . "GA526/09/0838" . " ribosomal" . "0"^^ . "3"^^ . "3"^^ . "Little attention has been paid to the development of inoculated arbuscular mycorrhizal fungi (AMF) in competition with the native AMF in soil. The aim of the proposed project is to quantitatively describe the coexistence of the inoculant and the native AMF in plant roots. This will be explored in microcosm experiments with strictly defined model conditions. Inoculum will be introduced into a soil colonised with a simulated community of three native AMF or into the same soil with its complete AMF community. Inoculation will be performed either with the native AMF or with non-native isolates of Glomus intraradices. Quantitative real-time PCR with species-specific primers will be adopted to selectively quantify the DNA of the native AMF. A quantitative PCR approach for the selective quantification of the native and the introduced isolates of G. intraradices will be developed. The results of the project will contribute to the fundamental knowledge on the development of AMF communities in plant roots and indicate how successful establishment of inoculant fungi could be achieved."@en . "1"^^ . " DNA" . . "Coexistence of native and inoculated arbuscular mycorrhizal fungi in the roots of host plants"@en . "http://www.isvav.cz/projectDetail.do?rowId=GA526/09/0838"^^ . "1"^^ . . . "2012-12-31+01:00"^^ . "2014-01-30+01:00"^^ . . . . . . . . . " mitochondrial" . . " community" . . " (mtDNA)" . "Vliv inokulace arbuskul\u00E1rn\u011B mykorhizn\u00EDmi houbami (AMH) na r\u016Fst rostlin je p\u0159edm\u011Btem mnoha studi\u00ED, rozvoji inokulovan\u00FDch hub v kompetici s nativn\u00EDmi houbami dan\u00E9 p\u016Fdy v\u0161ak zat\u00EDm byla v\u011Bnov\u00E1na jen mal\u00E1 pozornost. C\u00EDlem navrhovan\u00E9ho projektu je kvantitativn\u011B popsat koexistenci inokulovan\u00FDch a nativn\u00EDch AMH v ko\u0159enech rostlin na z\u00E1klad\u011B mikrokosmov\u00FDch pokus\u016F s p\u0159esn\u011B definovan\u00FDmi modelov\u00FDmi podm\u00EDnkami. Inokulum bude vn\u00E1\u0161eno do p\u016Fdy os\u00EDdlen\u00E9 simulovan\u00FDm spole\u010Denstvem t\u0159\u00ED nativn\u00EDch AMH nebo do stejn\u00E9 p\u016Fdy os\u00EDdlen\u00E9 jej\u00EDm kompletn\u00EDm spole\u010Denstvem AMH. K inokulaci budou pou\u017Eity bu\u010F nativn\u00ED houby nebo izol\u00E1ty druhu Glomus intraradices z jin\u00FDch lokalit. Pro stanoven\u00ED mno\u017Estv\u00ED DNA jednotliv\u00FDch nativn\u00EDch hub v ko\u0159enech rostlin bude p\u0159evzata metoda kvantitativn\u00ED real-time PCR s druhov\u011B specifick\u00FDmi primery. Postup pro selektivn\u00ED kvantifikaci DNA nativn\u00EDho a vn\u00E1\u0161en\u00FDch izol\u00E1t\u016F G. intraradices bude vyvinut v r\u00E1mci projektu. Projekt poskytne z\u00E1kladn\u00ED poznatky o rozvoji spole\u010Denstev AMH v ko\u0159enech rostlin a nazna\u010D\u00ED jak dos\u00E1hnout, aby se inokulovan\u00E9 AMH prosadily na pozad\u00ED nativn\u00EDho spole\u010Denstva." . " inoculation" . "The project contributed to the understanding of ecology of mycorrhizal fungi and may be used for further applied research. The results were published in one high quality (Applied and Environ. Microbiol. IF 3.8) and one Q2 IF journal, the former with a single dedication; two manuscripts are enclosed. The project contributed to student education, there were no problems in finance use."@en . . "2009-01-01+01:00"^^ . "Koexistence nativn\u00EDch a inokulovan\u00FDch arbuskul\u00E1rn\u011B mykorhizn\u00EDch hub v ko\u0159enech hostitelsk\u00FDch rostlin" . .