. . . . . . "Meiotic and mitotic division: role of the cytoplasm and the nucleus"@en . . "Mit\u00F3za a meioza p\u0159edstavuj\u00ED dva z\u00E1kladn\u00ED zp\u016Fsoby d\u011Blen\u00ED bun\u011Bk eukaryot\u016F. Mechanizmy vedouc\u00ED k\u00A0tomu, \u017Ee d\u011Blen\u00ED prob\u00EDh\u00E1 bu\u010F jako meioza nebo mit\u00F3za a ne naopak, nejsou dosud zn\u00E1my. Existuj\u00ED v\u00A0podstat\u011B dv\u011B teorie: prvn\u00ED p\u0159ikl\u00E1d\u00E1 rozhoduj\u00EDc\u00ED vliv j\u00E1dru (chromozom\u016Fm), druh\u00E1 pak cytoplazm\u011B. P\u0159\u00EDm\u00E9 d\u016Fkazy v\u0161ak dosud neexistuj\u00ED. C\u00EDlem p\u0159edkl\u00E1dan\u00E9ho projektu je zodpov\u011Bd\u011Bt tuto ot\u00E1zku u savc\u016F (my\u0161\u00ED), a to pomoc\u00ED reciprok\u00FDch p\u0159enos\u016F jader mezi mitotick\u00FDmi a meiotick\u00FDmi bu\u0148kami. Jako meiotick\u00E9 bu\u0148ky budou pou\u017Eity oocyty, \u010Dasn\u00E1 embrya budou pou\u017Eita jako zdroj mitotick\u00FDch bun\u011Bk. Hodnoceno bude chov\u00E1n\u00ED meiotick\u00E9ho j\u00E1dra v\u00A0mitotick\u00E9 cytoplazm\u011B a naopak, tzn. rozpad jadern\u00E9 membr\u00E1ny, kondenzace chromozom\u016F a pr\u016Fb\u011Bh d\u011Blen\u00ED. V\u00A0dal\u0161\u00ED \u010D\u00E1sti pokusu se zam\u011B\u0159\u00EDme na \u00FAlohu Wee 1 proteinu. V\u00FDsledky u ni\u017E\u0161\u00EDch \u017Eivo\u010Dich\u016F dokazuj\u00ED, \u017Ee absence Wee 1 proteinu u oocyt\u016F schopn\u00FDch zah\u00E1jit zr\u00E1n\u00ED a v\u00A0pr\u016Fb\u011Bhu prvn\u00EDho meiotick\u00E9ho d\u011Blen\u00ED m\u016F\u017Ee ur\u010Dovat vlastn\u00ED pr\u016Fb\u011Bh meiozy.Vyu\u017Eit\u00ED v\u00FDsledk\u016F p\u0159edpokl\u00E1d\u00E1me v\u00A0asistovan\u00E9 hum\u00E1nn\u00ED" . "GA524/02/0032" . . . . "Neuvedeno."@en . "C\u00EDlem projektu byla anal\u00FDza rozd\u00EDl\u016F mezi meiotick\u00FDm a mitotick\u00FDm bun\u011B\u010Dn\u00FDm cyklem, zejm\u00E9na pak st\u00E1dium p\u0159echodu meiozy na mit\u00F3zu. V\u00FDsledky z\u00EDskan\u00E9 b\u011Bhem \u0159e\u0161en\u00ED projektu dokazuj\u00ED, \u017Ee po oplozen\u00ED doch\u00E1z\u00ED k dramatick\u00FDm zm\u011Bn\u00E1m a transformaci genom\u016F obou pohla"@cs . "0"^^ . . . . "4"^^ . . "Mitotis and meiosis are two basic cell divisions in eukarytic cells. Mechanisms determining that meiosis occurs as meiosis and not mitosis and vice versa are still unknown. Basically there are two theories explaining this problem. First, the nucleus regulates the progress of cell division either as meiosis or mitosis. Second, the specific cytoplasmic factors present either in meiotic or mitotic cells determine the cell cycle progress. The aim of our project is to answer this question in mammals withthe help of reciprocal nuclear transfer between meiotic and mitotic cells and vice versa. Oocytes will be used as meiotic cells. Early embryos will be the source of mitotic cells. In reconstructed cells we will evaluate the nuclear envelope breakdown, chromosome condensation and the progress of meiosis (mitosis). It has been shown in lower animals (amphibians, starfish) that Wee 1 protein may play a key role in the regulation of meiosis. This protein cannot be detected in fully grown oocytes and is"@en . "4"^^ . . . "1"^^ . "1"^^ . "In our project we have analysed the differences between meiotic and mitotic cells, especially we were concentrated on the period of transition from meiosis into mitosis. The results obtained clearly show that after fertilization dramatic changes and tran"@en . . . "http://www.isvav.cz/projectDetail.do?rowId=GA524/02/0032"^^ . . . . "Meiotick\u00E9 a mitotick\u00E9 d\u011Blen\u00ED: \u00FAloha cytoplazmy a j\u00E1dra" . "2008-06-02+02:00"^^ .