"\u00DAloha Akt kin\u00E1zy pro regulaci G2/M f\u00E1ze bun\u011B\u010Dn\u00E9ho cyklu a z\u00EDsk\u00E1n\u00ED v\u00FDvojov\u00E9 kompetence oocyt\u016F" . . "3"^^ . "0"^^ . . . "3"^^ . . . "1"^^ . "In the proposed project regulation of G2/M checkpoint of meiotic cell cycle will be addressed in order to assess the relevance of these events for improvement of oocyte quality and developmental potency of early embryos. The data issued from this studywill also contribute to the understanding of cell cycle regulation. For this purpose the role of Akt kinase, an upstream regulator of p34cdc2 kinase, will be analysed during meiosis progression. To achieve this goal the experimental model of in vitromaturation of mouse, porcine and cattle oocytes will be used . Detailed analysis of expression, activation and localisation of Akt kinase and downstream Wee1 kinase family during meiosis progression will be performed. Dynamics of p34cdc2 kinaseactivation will be documented as well. Subsequently, specific inhibitors of Akt kinase activity will be used during in vitro culture in order to affect cytoplasmic maturation and to improve quality of oocytes. The impact of inhibition of Akt kinase upon"@en . "GA523/03/0857" . . . . "Navrhovan\u00FD projekt se zab\u00FDv\u00E1 regulac\u00ED G2/M f\u00E1ze meiotick\u00E9ho bun\u011B\u010Dn\u00E9ho cyklu s\u00A0c\u00EDlem vyu\u017E\u00EDt tyto poznatky pro zv\u00FD\u0161en\u00ED kvality zral\u00FDch oocyt\u016F a v\u00FDvojov\u00E9ho potenci\u00E1lu \u010Dasn\u00FDch embry\u00ED. \u00DAdaje z\u00EDskan\u00E9 p\u0159i \u0159e\u0161en\u00ED tohoto projektu tak\u00E9 p\u0159isp\u011Bj\u00ED k\u00A0pozn\u00E1n\u00ED regulacebun\u011B\u010Dn\u00E9ho cyklu. Za t\u00EDmto \u00FA\u010Delem bude v\u00A0pr\u016Fb\u011Bhu meiotick\u00E9ho zr\u00E1n\u00ED analyzov\u00E1na \u00FAloha kin\u00E1zy Akt (protein kin\u00E1za B), kter\u00E1 reguluje aktivitu p34cdc2 kin\u00E1zy. Pro \u0159e\u0161en\u00ED tohoto \u00FAkolu bude pou\u017E\u00EDv\u00E1n in vitro model meiotick\u00E9ho zr\u00E1n\u00ED oocyt\u016F my\u0161i, prasete askotu. V\u00A0z\u00E1vislosti na jednotliv\u00FDch st\u00E1di\u00EDch mei\u00F3zy bude provedena podrobn\u00E1 anal\u00FDza exprese, aktivace a lokalizace Akt kin\u00E1zy a j\u00ED pod\u0159\u00EDzen\u00FDch Wee1 kin\u00E1z, jako\u017E i bude podrobn\u011B dokumentov\u00E1na dynamika aktivity p34cdc2 kin\u00E1zy. V\u00A0n\u00E1vazn\u00FDch experimentechbudou p\u0159i kultivaci oocyt\u016F pou\u017Eity specifick\u00E9 inhibitory Akt kin\u00E1zy s\u00A0c\u00EDlem ovlivnit cytoplasmatick\u00E9 zr\u00E1n\u00ED a zv\u00FD\u0161it kvalitu oocyt\u016F. Bude provedena morfologick\u00E1 a biochemick\u00E1 anal\u00FDza \u00FA\u010Dinku inhibice kin\u00E1zy Akt v\u00A0pr\u016Fb\u011Bhu meiotick\u00E9ho zr\u00E1n\u00ED oocyt\u016F my\u0161i," . . "http://www.isvav.cz/projectDetail.do?rowId=GA523/03/0857"^^ . . "Byla provedena studie fosforylace a aktivace kin\u00E1zy Akt (alternativn\u00ED n\u00E1zev protein kin\u00E1za B - PKB) v pr\u016Fb\u011Bhu meiotick\u00E9ho zr\u00E1n\u00ED oocyt\u016F my\u0161\u00ED a prasete. U oocyt\u016F my\u0161i bylo zji\u0161t\u011Bno, \u017Ee b\u011Bhem znovuzah\u00E1jen\u00ED mei\u00F3zy v pr\u016Fb\u011Bhu rozpadu z\u00E1rode\u010Dn\u00E9ho v\u00E1\u010Dku (tj. j\u00E1d"@cs . . "0"^^ . . . "2009-01-15+01:00"^^ . "Neuvedeno."@en . . . . "Phosphorylation and activation of protein kinase Akt (protein kinase B) was evaluated during meiotic\u00A0maturation of mouse and porcine oocytes. At germinal vesicle breakdown (GVBD) of mouse oocytes the phosphorylation and activity of Akt/PKB was increased"@en . . . "The role of Akt kinase in the regulation of G2/M cell cycle transition and in the acquisition of developmental potency of oocytes"@en . . . .