"Neuvedeno."@en . "3"^^ . "Virus vr\u00E1s\u010Ditosti kmene jablon\u011B (apple stem pitting virus-ASPV) (typov\u00FD z\u00E1stupce skupiny Foveavirus) a jeho kmene viru \u017Eloutnut\u00ED \u017Eilek hru\u0161n\u011B (pear vein yellowing virus- PVYV) pat\u0159\u00ED k ekonomicky nejv\u00FDznamn\u011Bj\u0161\u00EDm vir\u016Fm jablon\u011B a hru\u0161n\u011B. Virus ASPV (PVYV) zp\u016Fsobuje onemocn\u011Bn\u00ED vr\u00E1s\u010Ditosti kmene resp. \u017Eloutnut\u00ED \u017Eilek list\u016F a vyskytuje se nej\u010Dast\u011Bji u komer\u010Dn\u00EDch odr\u016Fd. V \u010CR nebyla dosud vypracov\u00E1na expeditivn\u00ED diagnostick\u00E1 metoda ASPV, chyb\u00ED p\u0159edev\u0161\u00EDm SRS (Statn\u00ED rostlinolek\u00E1\u0159sk\u00E1 spr\u00E1va) pro testov\u00E1n\u00ED \u0161kolka\u0159sk\u00FDch aj. materi\u00E1l\u016F dov\u00E1\u017Een\u00FDch ze zahrani\u010D\u00ED i \u00DAKZ\u00DAZ (\u00DAst\u0159edn\u00ED kontroln\u00ED zku\u0161ebn\u00ED \u00FAstav zem\u011Bd\u011Blsk\u00FD) a SRS pro certifikaci \u0161kolka\u0159sk\u00FDch v\u00FDp\u011Bstk\u016F jablon\u00ED a hru\u0161n\u00ED. V zahrani\u010D\u00ED byly v posledn\u00EDch letech vypracov\u00E1ny spolehliv\u00E9 a p\u0159esn\u00E9 metody diagn\u00F3zy ASPV a PVYV pomoc\u00ED RT-PCR. V navrhovan\u00E9m projektu bude v prvn\u00ED f\u00E1zi studov\u00E1na detekce ASPV (PVYV) pomoc\u00ED bylinn\u00FDch a d\u0159evinn\u00FDch indik\u00E1torov\u00FDch rostlin. P\u0159\u00EDtomnost viru bude potvrzena elektronov\u00FDm mikroskopem, bude optimalizov\u00E1na metoda RT-PCR pro detekci" . . . . "0"^^ . . "3"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA522/00/D025"^^ . . "Apple stem pitting virus (ASPV) (type member of the genus Foveavirus) and its strain pear vein yellowing virus (PVYV) belongs to economically most significant virus in apple and pear production. ASPV (PVYV) cause the stem pitting and vein yellows diseasegenerally in commercial cultivars. The rapid diagnostics method of ASPV has not yet been elaborated in the Czech Republic. The absence of the method unables SRS (State Phytosanitary Administration) to test imported plant nursery materials, and \u00DAKZ\u00DAZ (Central Institute for Agricultural Supervision and Testing) & SRS to certify nursery apple and pear plants. Recently, RT-PCR was successfully applied for the diagnosis of ASPV (PVYV) in some foreign laboratories. The proposed project will be studied in the first stage: detection of ASPV (PVYV) by the indicator plants and the presence of the virus will be proved by electron microscopy. RT-PCR method will be elaborated for detection of ASPV (PVYV) in apple and pear orchards and a Czech isolate of ASPV an"@en . "Molekul\u00E1rn\u00ED a biologick\u00E1 diagnostika viru vr\u00E1s\u010Ditosti kmene jablon\u011B v \u010Cesk\u00E9 republice" . "1"^^ . "GA522/00/D025" . . . "Projekt %22Molekul\u00E1rn\u00ED a biologick\u00E1 diagnostika viru vr\u00E1s\u010Ditosti kmene jablon\u011B v \u010Cesk\u00E9 republice si kladl za c\u00EDl vypracovat a ov\u011B\u0159it expertn\u00ED diagnostickou metodu ASPV vyu\u017Eitelnou ve fytosanit\u00E1rn\u00ED praxi (SRS, diagnostick\u00E9 laborato\u0159e). K v\u00FDznamn\u00FDm d\u00EDl\u010D\u00EDm v\u00FD"@cs . "Molecular and biological diagnosis of apple stem pitting virus (ASPV) in the Czech Republic"@en . . . . . . . . . "0"^^ . "2008-05-19+02:00"^^ . .