. "\u0158e\u0161en\u00ED projektu prob\u011Bhlo podle pl\u00E1nu jak z hlediska odborn\u00E9ho tak i z hlediska \u010Derp\u00E1n\u00ED finan\u010Dn\u00EDch prost\u0159edk\u016F."@cs . . . . "2011-12-31+01:00"^^ . . . "Hlavn\u00EDmi c\u00EDli projektu je izolovat, sekven\u010Dn\u011B charakterizovat a funk\u010Dn\u011B analyzovat v heterologn\u00EDch syst\u00E9mech soubor transkrip\u010Dn\u00EDch regula\u010Dn\u00EDch faktor\u016F chmelu zejm\u00E9na z genov\u00FDch rodin Myb, bZIP a bHLH maj\u00EDc\u00EDch vztah k biosynt\u00E9ze lupulinu." . "chmel; H. lupulus; transkrip\u010Dn\u00ED faktory; prenylflavonoidy; ho\u0159k\u00E9 kyseliny; lupulin; transgeny"@en . . . " transkrip\u010Dn\u00ED faktory" . " prenylflavonoidy" . . "22"^^ . "22"^^ . . . " H. lupulus" . . . "2008-01-01+01:00"^^ . " ho\u0159k\u00E9 kyseliny" . "2011-04-16+02:00"^^ . "1"^^ . "1"^^ . "GA521/08/0740" . . "Molecular analysis of transcription factors of hop (H. lupulus L.) in relation to lupulin biosynthesis"@en . "chmel" . " lupulin" . "The project gone according to plan both in terms of expertise and in terms of disbursement of funds."@en . "Molekul\u00E1rn\u00ED anal\u00FDza transkrip\u010Dn\u00EDch faktor\u016F chmelu (H. lupulus L.) ve vztahu k biosynt\u00E9ze lupulinu" . "0"^^ . . . . "Previously we characterized an oligofamily chalconsynthase genes chs_H1 from hop, expressed in glandular tissue and\u00A0 involved in production of %22medicinal%22 prenylflavonoids and bitter acids important in brewing idustry. We also initialized an analysis of regulatory genes and characterized first Myb regulatory factors from hop using sequence homology approach. In the project proposed we aim to study a complex set of transcription factors (TFs) from R2R3Myb, bHLH and bZIP families, selected according to chs_H1 promoter binding sites.TFs will be isolated from cDNA libraries using tissue-specific probes and by means of promoter binding and protein:protein interaction systems. TFs interactions in vitro and in planta effects on metabolome will be analyzed in heterologous transgenotes and after transient expression in agroinfiltrated leaves. Quantification of TFs expression and sequence diversity analyses will be performed to compare high, and low-alpha hop genotypes. It is expected that the project contribute to analysis of hop metabolome regulation and TF-biotechnology."@en . "2015-03-02+01:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA521/08/0740"^^ . . . .