. . . "Pozi\u010Dn\u00ED klonov\u00E1n\u00ED v obrovsk\u00E9m allopolyploidn\u00EDm genomu p\u0161enice set\u00E9 bylo doned\u00E1vna pova\u017Eov\u00E1no za nemo\u017En\u00E9 kv\u016Fli p\u0159\u00EDtomnosti t\u0159\u00ED homeologn\u00EDch genom\u016F a zna\u010Dn\u00E9mu mno\u017Estv\u00ED repetitivn\u00EDch sekvenc\u00ED. Jedna ze strategi\u00ED vedouc\u00EDch k dosa\u017Een\u00ED tohoto c\u00EDle je zalo\u017Eena na vyu\u017Eit\u00ED genomick\u00FDch materi\u00E1l\u016F vytvo\u0159en\u00FDch pouze z men\u0161\u00EDch \u010D\u00E1st\u00ED genomu. Navrhujeme p\u0159ipravit dv\u011B subgenomov\u00E9 knihovny klonovan\u00E9 ve vektoru BAC, specifick\u00E9 pro kr\u00E1tk\u00E9 a dlouh\u00E9 rameno chromoz\u00F3mu 7D p\u0161enice. Tyto knihovny budou neoceniteln\u00FDm zdrojem p\u0159i tvorb\u011B fyzick\u00FDch map chromoz\u00F3mu 7D a pozi\u010Dn\u00EDm klonov\u00E1n\u00ED n\u011Bkolika agronomicky v\u00FDznamn\u00FDch gen\u016F z tohoto chromoz\u00F3mu, mimo jin\u00E9 gen\u016F pro rezistence ke \u0161k\u016Fdc\u016Fm Schizaphis graminum a Diuraphis noxia a patogen\u016Fm Blumeria graminis, Puccinia triticina, P. striiformis, P. graminis f. sp. tritici nebo Mycosphaerella graminicola. Projekt bude zam\u011B\u0159en na pozi\u010Dn\u00ED klonov\u00E1n\u00ED genu Dnx pro rezistenci k m\u0161ici D. noxia. Sou\u010D\u00E1st\u00ED bude vytvo\u0159en\u00ED hust\u00E9 genetick\u00E9 mapy pro oblast genu Dnx a sestaven\u00ED kontigu BAC klon\u016F" . . "."@en . "http://www.isvav.cz/projectDetail.do?rowId=GA521/07/1573"^^ . . "GA521/07/1573" . "P\u0159\u00EDprava materi\u00E1l\u016F pro genomiku p\u0161enice a pozi\u010Dn\u00ED klonov\u00E1n\u00ED gen\u016F pro rezistence na chromoz\u00F3mu 7D" . "2007-01-01+01:00"^^ . . . . . . . "0"^^ . . "1"^^ . . . . "Triticum aestivum" . "2015-01-22+01:00"^^ . "0"^^ . . . "V\u00FDsledky uveden\u00E9 v z\u00E1v\u011Bre\u010Dn\u00E9 zpr\u00E1v\u011B ukazuj\u00ED na spln\u011Bn\u00ED c\u00EDl\u016F projektu. V\u00FDstupem je jedna kapitola v knize a jeden \u010Dl\u00E1nek v tisku a 3 rukopisy."@cs . "2009-12-31+01:00"^^ . "Positional cloning in a huge allopolyploid genome of bread wheat was considered infeasible, mainly due to presence of three homoeologous genomes and large amounts of repetitive sequences. One of strategies enabling to reach this target is based on the use of genomic resources derived from parts of the hexaploid genome. Here we propose construction of two subgenomic BAC libraries specific for short and long arm of chromosome 7D. They will be invaluable resource for creation of physical maps of chromosome 7D and map-based cloning of several agronomically important genes from chromosome 7D of wheat, including genes conferring resistance to green bug, powdery mildew, stem rust, leaf rust, stripe rust, Septoria tritici blotch and Russian wheat aphid. The project will focus on positional cloning of gene Dnx for resistance to Russian wheat aphid (RWA) by creating high-density genetic map for the Dnx region and BAC contig spanning this region. Further, a pooled genomic BAC library will be"@en . " positional cloning" . "Creation of resources for wheat genomics and map-based cloning of resistance genes from chromosome 7D"@en . " chromosome-specific libraries" . "6"^^ . "Triticum aestivum; BAC; chromosome-specific libraries; positional cloning; Dnx"@en . "6"^^ . . " BAC" . . . "2009-04-22+02:00"^^ .