. "Molekul\u00E1rn\u00ED evoluce a funk\u010Dn\u00ED anal\u00FDza komponent rostlinn\u00FDch telomer a telomer\u00E1z" . . . "2007-05-02+02:00"^^ . . " telomerase" . "50"^^ . . "telomeres" . . . "50"^^ . "\u0158e\u0161en\u00ED projektu p\u0159ineslo p\u016Fvodn\u00ED v\u00FDsledky v oblasti anal\u00FDzy struktury rostlinn\u00FDch telomer a telomer\u00E1z a zejm\u00E9na molekul\u00E1rn\u00EDch p\u0159\u00ED\u010Din jejich evolu\u010Dn\u00EDch zm\u011Bn. Byly izolov\u00E1ny a charakterizov\u00E1ny geny pro katalytickou podjednotku telomer\u00E1z, syntetizuj\u00EDc\u00EDch bu"@cs . . "telomeres; telomerase; telomere-binding proteins; chromatin; plants"@en . . "http://www.isvav.cz/projectDetail.do?rowId=GA521/05/0055"^^ . . . "GA521/05/0055" . . "Molecular evolution and functional analysis of components of plant telomeres and telomerases"@en . . "2007-12-31+01:00"^^ . . "2008-12-16+01:00"^^ . "0"^^ . "Project solution produced results in the field of structural analysis of plant telomeres and telomerases and namely on molecular causes of their evolutionary changes. Novel genes coding for catalytic telomerase subunit were isolated and characterised in"@en . "2005-01-01+01:00"^^ . "0"^^ . . "1"^^ . . "Telomery jsou nukleoproteinov\u00E9 struktury, kter\u00E9 chr\u00E1n\u00ED konce chromosom\u016F p\u0159ed degradac\u00ED a f\u00FAzemi. Mnoho organism\u016F u\u017E\u00EDv\u00E1 telomer\u00E1zu, obsahuj\u00EDc\u00ED telomerovou reversn\u00ED transkript\u00E1zu (TERT) a telomer\u00E1zovou RNA (TR), k synt\u00E9ze konc\u016F chromosom\u00E1ln\u00ED DNA zkr\u00E1cen\u00FDchp\u0159i replikaci. Zjistili jsme, \u017Ee u rostlin \u0159\u00E1du Asparagales je druhov\u00E1 divergence spojen\u00E1 s evoluc\u00ED nov\u00E9ho typu telomer. Krom\u011B skupiny rostlin (i) (nap\u0159. orchideje) s typick\u00FDmi telomerami zalo\u017Een\u00FDmi na motivech [TTTAGGG]n existuje skupina (ii) (nap\u0159. Asparagus), u n\u00ED\u017E v d\u016Fsledku mutace(-\u00ED) doch\u00E1z\u00ED k synt\u00E9ze telomer lidsk\u00E9ho typu [TTAGGG]n. T\u0159et\u00ED skupina rostlin (iii) (nap\u0159. Allium) nesyntetizuje \u017E\u00E1dn\u00E9 zn\u00E1m\u00E9 telomerov\u00E9 repetice a jej\u00ED telomeriza\u010Dn\u00ED mechanismus je zna\u010Dn\u011B odli\u0161n\u00FD. Variace, kter\u00E9 se vyskytuj\u00ED u t\u011Bchto p\u0159\u00EDbuzn\u00FDch rostlin umo\u017E\u0148uj\u00ED \u0159e\u0161it kl\u00ED\u010Dov\u00E9 ot\u00E1zky o evoluci telomer a telomer\u00E1zy a o funkci jednotliv\u00FDch sou\u010D\u00E1st\u00ED telomeriza\u010Dn\u00EDho syst\u00E9mu. C\u00EDle projektu zahrnuj\u00ED 1) izolaci a komparativn\u00ED anal\u00FDzu TR a TERT podjednotek telomer\u00E1z u druh\u016F" . " telomere-binding proteins" . . " chromatin" . . . "Telomeres are nucleoprotein structures protecting chromosome ends from degradation and fusions. Many organisms use telomerase, comprising telomere reverse transcriptase (TERT) and telomerase RNA (TR), to replenish ends of chromosomal DNA lost at replication. We found in Asparagales that species divergence is associated with the evolution of novel types of telomeres. In addition to group (i) plants (e.g. orchids) with typical plant telomeres based on [TTTAGGG]n motifs, the group (ii) plants (e.g.Asparagus) exists in which mutation(s) results in synthesis of human-like [TTAGGG]n telomeres. The last group (iii) plants (e.g. Allium) do not synthesize any known telomeric repeat and their telomerization strategy must be different. The variations thatoccur in these related plants enable us to address key questions about telomere and telomerase evolution and function of components of telomeric machinery. Project objectives include 1) isolation and comparative analysis of TERT and TR telomerase"@en .