. "1"^^ . . . . . "The effects of genetic and DNA damaging factors upon developmental potent of germ and embryonic cells"@en . "http://www.isvav.cz/projectDetail.do?rowId=GA505/95/0357"^^ . . . "0"^^ . "GA505/95/0357" . . . . "Sou\u010Dasn\u00E9 poznatky o mechanismech reakce na po\u0161kozen\u00ED DNA jsou omezeny p\u0159ev\u00E1\u017En\u011B na bu\u0148ky prokaryot, ni\u017E\u0161\u00EDch eukaryot a somatic\u00E9 bu\u0148ky odvozen\u00E9 z vy\u0161\u0161\u00EDch eukaryot. V navrhovan\u00E9m projektu budeme studovat tyto mechanismy u vyv\u00EDjej\u00EDc\u00EDch se sav\u010D\u00EDch oocyt\u016F a u bun\u011Bk \u010Dasn\u00FDch embry\u00ED. Bude testov\u00E1na hypot\u00E9za, \u017Ee produkt genu p53 hraje roli ve v\u00FDvoji my\u0161\u00EDch oocyt\u016F a embryon\u00E1ln\u00EDch kmenov\u00FDch bun\u011Bk za norm\u00E1ln\u00EDch i genom destabilizuj\u00EDc\u00EDch podm\u00EDnek (chromosom\u00E1ln\u00ED translokace, ionizuj\u00EDc\u00ED a UV-z\u00E1\u0159en\u00ED). Bude pou\u017Eit jedinen\u00FD experiment\u00E1ln\u00ED model my\u0161i nesouc\u00ED null mutaci v p53 genu. Budou porovn\u00E1ny parametry v\u00FDvoje a bun\u011B\u010Dn\u00E9ho cyklu u norm\u00E1ln\u00EDch my\u0161\u00ED a jejich geneticky identick\u00FDch prot\u011Bj\u0161k\u016F, postr\u00E1daj\u00EDc\u00EDch funk\u010Dn\u00ED p53 gen. D\u00E1le budou identifikov\u00E1ny geny, kter\u00E9 mohou m\u00EDt fnk\u010Dn\u00ED vztah k p53. K tomu budou pou\u017Eity metody reverzn\u00ED polymer\u00E1zov\u00E9 \u0159et\u011Bzov\u00E9 reakce (RT PCR) s n\u00E1hodn\u00FDmi primery a konstrukce cDNA knihoven k odhalen\u00ED rozd\u00EDl\u016F v expresi genomu." . . "Vliv genetick\u00FDch a DNA po\u0161kozuj\u00EDc\u00EDch faktor\u016F na v\u00FDvojovou schopnost z\u00E1rode\u010Dn\u00FDch a embryon\u00E1ln\u00EDch bun\u011Bk" . . "2"^^ . . "2"^^ . "1"^^ .