"2011-04-16+02:00"^^ . "T. pallidum ssp. pallidum" . "The aim of this proposal is to study the genomes of pathogenic spirochetes of the genus Treponema using methods of comparative genomics including the whole genome fingerprinting method (WGF), comparative genome sequencing (CGS) approach and Chromatin Immunoprecipitation method (ChIP-chip). Genomes of five strains are intended to be sequenced using CGS strategy, i.e. T. pallidum ssp. pallidum (Chicago), T. pallidum ssp. pertenue (Samoan D and Gauthier) and T. pallidum ssp. endemicum (Iraq B and Bosnia A). We intend to determine additional transcriptomes of two strains of T. pallidum ssp. pertenue and compared them to the transcriptome of T. pallidum ssp. pallidum (Nichols). The ChIP-chip method will be used for identification of transcription factor binding sites in T. pallidum ssp. pallidum (Nichols). Differences in the genome structure and in the transcriptional profiles will be related to the different pathogenic features of studied organisms. Promoter identifications will be used for elucidation of the transcriptional regulatory network."@en . "Komparativn\u00ED genomov\u00E9 sekvencov\u00E1n\u00ED patogenn\u00EDch treponem a transkriptomov\u00E1 anal\u00FDza T. pallidum ssp. pallidum" . "2015-03-02+01:00"^^ . "2007-01-01+01:00"^^ . . . "Genomics of Treponema: comparative genome sequencing of pathogenic treponemes and transcriptome analysis of T. pallidum ssp. pallidum"@en . "C\u00EDlem tohoto v\u00FDzkumn\u00E9ho projektu je studium genom\u016F patogenn\u00EDch spirochet rodu Treponema s pou\u017Eit\u00EDm metod komparativn\u00ED a funk\u010Dn\u00ED genomiky. Tyto metody zahrnuj\u00ED techniku whole genome fingerprinting (WGF), komparativn\u00ED genomov\u00E9 sekvencov\u00E1n\u00ED (CGS) a metodu chromativ\u00E9 imunoprecipitace na \u010Dipech (ChIP-chip). Pomoc\u00ED CGS pl\u00E1nujeme sekvencovat genomy 5 kmen\u016F, t.j. T. pallidum ssp. pallidum (Chicago), T. pallidum ssp. pertenue (kmeny Samoan D a Gauthier) a T. pallidum ssp. endemicum (kmeny Iraq B a Bosnia A). Pl\u00E1nujeme tak\u00E9 vy\u0161et\u0159it transkriptomy dvou kmen\u016F T. pallidum ssp. pertenue a porovnat je s transkriptomem T. pallidum ssp. pallidum kmene Nichols. Technika\u00A0 ChIP-chip bude pou\u017Eita pro identifikaci vazebn\u00FDch DNA m\u00EDst (promotor\u016F) transkrip\u010Dn\u00EDch faktor\u016F\u00A0 v genomu T. pallidum ssp. pallidum Nichols. Nalezen\u00E9 rozd\u00EDly v genomov\u00E9 struktu\u0159e \u00A0a trankrip\u010Dn\u00EDch profilech budou porovn\u00E1ny s odli\u0161n\u00FDm patogenn\u00EDm potenci\u00E1lem vy\u0161et\u0159en\u00FDch treponem. Identifice promotor\u016F (spolu s transkriptomov\u00FDmi daty) bude pou\u017Eita pro n\u00E1vrh operonov\u00E9 struktury a pro zmapov\u00E1n\u00ED transkrip\u010Dn\u00ED \u00FArovn\u011B regulace genov\u00E9 exprese u treponem." . . . . . . . "34"^^ . "T. pallidum ssp. pallidum, genomov\u00E9 sekvenov\u00E1n\u00ED, profilov\u00E1n\u00ED genov\u00E9 exprese"@en . . "34"^^ . . "GA310/07/0321" . "2011-12-31+01:00"^^ . " genomov\u00E9 sekvenov\u00E1n\u00ED" . . . "http://www.isvav.cz/projectDetail.do?rowId=GA310/07/0321"^^ . . . . . . "\u0158e\u0161en\u00ED projektu prob\u011Bhlo v\u00FDte\u010Dn\u011B, jak z hlediska odborn\u00E9ho tak i z hlediska \u010Derp\u00E1n\u00ED finan\u010Dn\u00EDch prost\u0159edk\u016F."@cs . "0"^^ . "1"^^ . "The project was excellent, both in terms of expertise and in terms of disbursement of funds."@en . "0"^^ . . .