. . . "GA309/08/0292" . . . . . . . . . "Role of oxidative stress in brain injury following status epilepticus induced in immature rats"@en . . . "0"^^ . . . "The aim of the present project, employing the model of status epilepticus induced in immature rats by administration of DL-homocysteic acid (HCA), is to investigate the role of oxidative stress in seizure-induced brain injury, with the specific focus on:(1) free radical production (2) characterization of endogenous antioxidant defense systems and (3) revealing potential protective treatments. Several methodical approaches will be used:\u00A0 aconitase activity as a marker of\u00A0O2-. production, O2-. detection by Amplex Red and by in situ imaging method employing hydroethidium, immunohistochemical detection of nitrotyrosine as a marker of peroxynitrite, mitochondrial function by measurement of respiratory chain complex activities and brain energy status. Activity of superoxide dismutase, catalase, glutathione-peroxidase and glutathione levels will serve to characterize antioxidant systems. Brain injury will be evaluated by Nissl and Fluoro-Jade B staining. In order to reveal the share of seizure activity"@en . . . "2008-01-01+01:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA309/08/0292"^^ . . "Role of oxidative stress and mitochondrial dysfunction in brain damage was studied on the model of seizures induced in immature rats by DL-homocysteic acid. It was demonstrated that a marked decrease of mitochondrial complex I activity persists up to 5 weeks after the end of seizures and it is not due to the changes in its content. It is accompanied by significant increases of oxidative damage ma"@en . "1"^^ . "2010-04-16+02:00"^^ . "C\u00EDlem projektu, zalo\u017Een\u00E9ho na modelu status epilepticus vyvolan\u00E9m u ml\u00E1\u010Fat laboratorn\u00EDho potkana pod\u00E1n\u00EDm kys. homocysteov\u00E9, je studovat \u00FAlohu oxidativn\u00EDho stresu v po\u0161kozen\u00ED mozku vyvolan\u00E9m\u00A0k\u0159e\u010Dovou aktivitou,\u00A0\u00A0se zam\u011B\u0159en\u00EDm na: (1) produkci voln\u00FDch radik\u00E1l\u016F (2) charakterizaci endogenn\u00EDch antioxida\u010Dn\u00EDch obrann\u00FDch syst\u00E9m\u016F a (3) objasn\u011Bn\u00ED mo\u017En\u00FDch protek\u010Dn\u00EDch mechanism\u016F. Bude pou\u017Eito n\u011Bkolika metodick\u00FDch p\u0159\u00EDstup\u016F: sledov\u00E1n\u00ED aktivity akonit\u00E1zy jako ukazatele produkce O2-. radik\u00E1l\u016F, detekce O2-. pomoc\u00ED Amplex Red a pou\u017Eit\u00EDm in situ metody vyu\u017E\u00EDvaj\u00EDc\u00ED hydroethidium, immunohistochemick\u00E1 detekce nitrotyrosinu jako ukazatele peroxynitritu, studium mitochondri\u00E1ln\u00ED funkce m\u011B\u0159en\u00EDm aktivity komplex\u016F respira\u010Dn\u00EDho \u0159et\u011Bzce a energetick\u00E9ho stavu mozku. Aktivity superoxid dismut\u00E1zy, katal\u00E1zy, glutathionperoxid\u00E1zy a hladiny glutathionu budou slou\u017Eit pro charakterizaci antioxida\u010Dn\u00EDch syst\u00E9m\u016F. Po\u0161kozen\u00ED mozku bude hodnoceno barven\u00EDm podle Nissla a Fluoro-Jade B. Budou provedeny rovn\u011B\u017E pokusy na \u0159ezech mozkov\u00E9 tk\u00E1n\u011B" . "\u00DAloha oxidativn\u00EDho stresu a mitochondri\u00E1ln\u00ED dysfunkce v po\u0161kozen\u00ED mozku byla studov\u00E1na na modelu k\u0159e\u010D\u00ED vyvolan\u00FDch u ml\u00E1\u010Fat laboratorn\u00EDho potkana kyselinou homocysteovou. Bylo zji\u0161t\u011Bno, \u017Ee v\u00FDrazn\u00E9 sn\u00ED\u017Een\u00ED aktivity mitochondri\u00E1ln\u00EDho komplexu I p\u0159etrv\u00E1v\u00E1 a\u017E 5 t\u00FDdn\u016F po ukon\u010Den\u00ED k\u0159e\u010D\u00ED a nen\u00ED zp\u016Fsobeno zm\u011Bnami v jeho obsahu. Jedn\u00E1 se z\u0159ejm\u011B o oxidativn\u00ED modifikaci enzymov\u00E9 aktivity. Inhibice"@cs . "2010-12-31+01:00"^^ . "\u00DAloha oxidativn\u00EDho stresu v po\u0161kozen\u00ED mozku n\u00E1sleduj\u00EDc\u00EDho po status epilepticus vyvolan\u00E9m u ml\u00E1\u010Fat laboratorn\u00EDho potkana" . "0"^^ . . "immature rats-homocysteic acid-status epilepticus-oxidative stress-brain damage-protection"@en . . "7"^^ . "2015-02-09+01:00"^^ . "7"^^ .