. . "0"^^ . "Neuvedeno."@en . "V\u00A0pr\u016Fb\u011Bhu tohoto grantov\u00E9ho projektu byly studov\u00E1ny zejm\u00E9na mechanismy vzniku patologick\u00FDch bolestiv\u00FDch stav\u016F hyperalgesie a allodynie na m\u00ED\u0161n\u00ED \u00FArovni. V\u00A0pr\u016Fb\u011Bhu \u0159e\u0161en\u00ED byly vyu\u017E\u00EDv\u00E1ny behavior\u00E1ln\u00ED, immunohistologick\u00E9, optick\u00E9 a elektrofyziologick\u00E9 metody"@cs . . . "Cellular and molecular mechanisms of nociception at spinal cord level."@en . . . "1"^^ . . . "Spinothalamick\u00FD tract je pova\u017Eov\u00E1n za\u00A0hlavn\u00ED vzestupnou dr\u00E1hu zapojenou do p\u0159enosu akutn\u00ED a chronick\u00E9 bolesti. V\u00FDsledky z posledn\u00EDch let ukazuj\u00ED, \u017Ee jako jeden z hlavn\u00EDch mechanism\u016F allodynie, hyperalgesie a jin\u00FDch chronick\u00FDch bolestiv\u00FDch stav\u016F m\u016F\u017Eeslou\u017Eit sensitizace spinothalamick\u00FDch neuron\u016F. V sou\u010Dastnosti je ale jen velmi m\u00E1lo zn\u00E1mo o vlastnostech synaptick\u00E9ho p\u0159enosu na t\u011Bchto neuronech. V tomto grantov\u00E9m projektu navrhujeme studovat vlastnosti glutam\u00E1tov\u00FDch receptor\u016F a mechanismy sensitizacespinothalamick\u00FDch neuron\u016F in vitro. Technikou ter\u010D\u00EDkov\u00E9ho z\u00E1mku budou studov\u00E1ny excita\u010Dn\u00ED postsynaptick\u00E9 proudy a vlastnosti jednotliv\u00FDch glutam\u00E1tov\u00FDch kan\u00E1l\u016F na retrogr\u00E1dn\u011B ozna\u010Den\u00FDch spinothalamick\u00FDch neuronech pod p\u0159\u00EDmou vizu\u00E1ln\u00ED kontrolou v m\u00ED\u0161n\u00EDch\u0159\u00EDzc\u00EDch. Vlastnosti glutam\u00E1tov\u00FDch receptor\u016F budou porovn\u00E1v\u00E1ny za kontroln\u00EDch podm\u00EDnek a po navozen\u00ED sensitizace. Studium mechanism\u016F sensitizace se bude soust\u0159edit zejm\u00E9na na \u00FAlohu fosforylace protein\u016F, kter\u00E1 hraje kl\u00ED\u010Dovou \u00FAlohu p\u0159i synaptick\u00E9 modulaci" . "GA309/03/0752" . . "Spinothalamic tract (STT) is considered to be one of the major ascending pathways involved in the transmission of acute and chronic pain. Sensitization of STT neurons is thought to be one of the important mechanisms underlying changes in pain perceptionsuch as allodynia, hyperalgesia and possibly various chronic pain syndromes. However, very little is known about the properties of synaptic transmission on these neurons. In this grant, we propose to study the properties of glutamate receptors in STTneurons and the mechanisms of STT neuronal sensitization. Postsynaptic currents and single channel properties of glutamate receptors will be studied using patch clamp technique in STT neurons retrogradelly labeled and visually identified in spinal cordslices. The properties of the glutamate receptors will be characterized under control conditions and after induction of sensitization. We will focus on the role of protein phosphorylation in this process, as it was shown to be a key factor in long-term"@en . . . . "0"^^ . "Bun\u011B\u010Dn\u00E9 a molekul\u00E1rn\u00ED mechanizmy p\u0159enosu bolestiv\u00FDch sign\u00E1l\u016F na m\u00ED\u0161n\u00ED \u00FArovni." . . "6"^^ . . "6"^^ . "In this grant project\u00A0 mechanisms of pathological pain states characterized by hyperlagesia and allodynia were studied. Behavioral, immunohistological, optical and electrophysiological methods were used to study the mechanisms of nociception under contro"@en . . . . . . "2009-01-15+01:00"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA309/03/0752"^^ . .