. . . "The aim of the project is the evaluation of the influence of the polymorphism of the gene for endothelin l (EDN1) from the point of view of the origin and development of the essential hypertension in man. Subjects will be charakterised regarding to the presence or absence of usual (= on the gene polymorphism participating) alleles of EDN l locus. Associations between the phenotype (the parameters of the 24hrs measured blood pressure) and the corresponding individual genotypes will be tested by means of multivariational statistical methods (CSS). Two Taq1 polymorphic places in EDN1 gene will be examinated in position 8000 (intron 4) and 9587 (exon 5). PCR (polymerase chain reaction) methods will be developed to a given problem for replacing the current RFLP (restriction fragments lenght polymorphism) technic. Further a short tandem repeat polymorphism OT EDN1 will be evaluated. The phenotype (blood pressure) will be measured in the connection with the study of genetics by the 24hrs non-invasive"@en . . "0"^^ . . . "17"^^ . . "C\u00EDlem projektu je zhodnocen\u00ED polymorfismu genu endotelin 1 (EDN1) z hlediska vzniku a rozvoje esenci\u00E1ln\u00ED hypertenze u \u010Dlov\u011Bka. Pokusn\u00E9 osoby budou charakterizov\u00E1ny vzhledem k p\u0159\u00EDtomnosti nebo nep\u0159\u00EDtomnosti \u010Dast\u00FDch (tedy na polymorfismu genu se \u00FA\u010Dastn\u00EDc\u00EDch) alel lokusu pro EDN1. Prost\u0159ednictv\u00EDm multivaria\u010Dn\u00EDch statistick\u00FDch metod budou vyhodnoceny asociace mezi fenotypem (parametry 24 hod. m\u011B\u0159en\u00E9ho krevn\u00EDho tlaku) a odpov\u00EDdaj\u00EDc\u00EDmi individu\u00E1ln\u00EDmi genotypy. Budou vy\u0161et\u0159ov\u00E1na dv\u011B polymorfn\u00ED m\u00EDsta pro restrikt\u00E1zu Taq1 v pozici 8000 (intron 4) a 9587 (exon 5) genu pro preproendotelin 1. Bude vyvinuta metoda PCR (polymer\u00E1zov\u00E1 \u0159et\u011Bzov\u00E1 reakce), kter\u00E1 nahrad\u00ED pro dan\u00FD polymorfismus dosavadn\u00ED metodu RFLP (polymorfismus d\u00E9lky restrik\u010Dn\u00EDch fragment\u016F). D\u00E1le se budehodnotit polymorfismus kr\u00E1tk\u00FDch tandemov\u00FDch repetic\u00ED endotelinov\u00E9ho genu. Fenotyp (krevn\u00ED tlak) se bude m\u011B\u0159it paraleln\u011B s genetickou studi\u00ED pomoc\u00ED 24 hodinov\u00E9ho monitoringu neinvasivn\u00ED metodou: lze o\u010Dek\u00E1vat, \u017Ee toto m\u011B\u0159en\u00ED poskytne mnohem detailn\u011Bj\u0161\u00ED in" . "17"^^ . "Polymorfismus genu pro endotelin - 1 (EDN 1) u esenci\u00E1ln\u00ED hypertense" . . . . "Z\u00E1m\u011Bry anotace projektu byly spln\u011Bny. Zavedenou metodou sledov\u00E1n v\u00FDskyt polymorfismu pro ET1 u esenci\u00E1ln\u00ED hypertenze. \u0158e\u0161itel\u00E9 prok\u00E1zali signifikantn\u00ED rozd\u00EDly v alelick\u00FDch d\u00E9lk\u00E1ch CA repeatu v Z-oblasti genu pro ET1 mezi normotoniky a hypertoniky s \u010Dast\u011B"@cs . . "http://www.isvav.cz/projectDetail.do?rowId=GA306/96/0099"^^ . . . "0"^^ . . . "Polymorphism of endothelin - 1 gene (EDN 1) in essential hypertension"@en . "1"^^ . "GA306/96/0099" .