"http://www.isvav.cz/projectDetail.do?rowId=GA305/99/0695"^^ . "Neuvedeno."@en . . . . . "Affection of DNA conformation by metal-based anticancer drugs. Relations to the development of new cytostatics"@en . "1"^^ . "This project is aimed at complex studies of the mechanism of anticancer activity of the metal-based compounds and is based on recent progress reached in this field in the applicant's laboratory. The goal of these studies is mainly to develop a systematic and complex theoretical background to the synthesis of metal complexes with unique DNA binding activities capable of overcoming the problem of cellular resistance to metal-based cytostatics already used in clinic and of their limited activity against the most common tumors. An important aspect of this approach is to find the DNA adduct of platinum or ruthenium complexes most likely relevant to their antitumor effect and the factors controlling its formation. Another important aspect is to examine and compare the mechanism of action of metal complexes structurally distinct from platinum anticancer drugs used in the clinic. Specific aims of this project are to: 1. Determine the effect of DNA topology on bindin"@en . . . "Ve t\u0159et\u00EDm roce \u0159e\u0161en\u00ED se usilovalo o dokon\u010Den\u00ED termodynamick\u00E9 anal\u00FDzy stability DNA obsahuj\u00EDc\u00ED vybran\u00E9 adukty platinov\u00FDch cytostatik modifikac\u00ED dvou\u0159et\u011Bzov\u00E9 DNA protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDmi komplexy in vitro. \u00DAdaje \u0159e\u0161itele tak, jak je uvedeno v Z\u00E1v\u011Bre\u010Dn\u00E9 kart"@cs . "0"^^ . . "0"^^ . . . . . "Tento projekt je zam\u011B\u0159en na komplexn\u00ED studie mechanismu ptotin\u00E1dorov\u00E9 \u00FA\u010Dinnosti komplex\u016F kov\u016F a je zalo\u017Een na pokroku dosa\u017Een\u00E9m v laborato\u0159i navrhovatele v t\u00E9to v\u011Bdn\u00ED oblasti. C\u00EDlem studi\u00ED popsan\u00FDch v tomto projektu je vyvinout systematick\u00E9 a komplexn\u00ED teoretick\u00E9 z\u00E1zem\u00ED pro synt\u00E9zu komplex\u016F kov\u016F s unik\u00E1tn\u00EDmi vazebn\u00FDmi vlastmostmi v\u016F\u010Di DNA, kter\u00E9 jsou schopn\u00E9 p\u0159ekonat jednak probl\u00E9my z\u00EDskan\u00E9 bun\u011B\u010Dn\u00E9 rezistence k cytostatik\u016Fm odvozen\u00FDm od komplex\u016F kov\u016F a ji\u017E vyu\u017E\u00EDvan\u00FDm v klinick\u00E9 praxi, jednak probl\u00E9my souvisej\u00EDc\u00ED s jejich omezenou aktivitou v nejb\u011B\u017En\u011Bj\u0161\u00EDch n\u00E1dorech (n\u00E1dorech st\u0159ev a prsu) a vedlej\u0161\u00EDmi \u00FA\u010Dinky. D\u016Fel\u017Eit\u00FDm aspektem tohoto v\u00FDzkumu je tak\u00E9 popsat po\u0161kozen\u00ED DNA vyvolan\u00E1 komplexy platiny a rhutenia, kter\u00E1 vedou k protin\u00E1dorov\u00E9mu efektu a faktory ovliv\u0148uj\u00EDc\u00ED jejich vznik. D\u016Fle\u017Eit\u00FD ja tak\u00E9 n\u00E1vrh na zkoum\u00E1n\u00ED a srovn\u00E1n\u00ED mechanismu p\u016Fsoben\u00ED komplex\u016F kov\u016F strukturn\u011B odli\u0161n\u00FDch od platinov\u00FDch cytostatik ji\u017E pou\u017E\u00EDvan\u00FDch v klinick\u00E9 praxi. Specifick\u00E9 c\u00EDle tohoto projektu jsou: l)stanovit efekt topo" . "GA305/99/0695" . "Ovlivn\u011Bn\u00ED konformace DNA protin\u00E1dorov\u011B \u00FA\u010Dinn\u00FDmi komplexy kov\u016F. Vztah k v\u00FDvoji nov\u00FDch cytostatik." . "39"^^ . "2003-04-01+02:00"^^ . . "39"^^ . . . . . .