"2009-01-01+01:00"^^ . . "neuroregenerace; Sonic hedgehog; Wnt-7a; proliferace; differenciace; astrocyty; NG2-glie; neur\u00E1ln\u00ED kmenov\u00E9"@en . . "Indukce neuro- a gliogeneze po ischemick\u00E9m po\u0161kozen\u00ED mozku potkana-\u00FAloha morfogen\u016F a r\u016Fstov\u00FDch faktor\u016F v pr\u016Fb\u011Bhu regenerace nervov\u00E9 tk\u00E1n\u011B" . . " Wnt-7a" . . "8"^^ . "8"^^ . . " Sonic hedgehog" . . . "0"^^ . "Sonic hedgehog (Shh) and Wnt signalling are both required for the maintenance of stem cell niches in the postnatal subventricular zone and for the proliferation of neural stem/progenitor cells in the mature hippocampus. The aim of this project is to\u00A0elicidate the effect of two morphogenes, Shh and Wnt-7a, on neuro- and gliogenesis using neural stem/progenitor cells that express either Shh or Wnt-7a. Their role in neural stem/progenitor proliferation and differentiation will be studied in vitro and after cell transplantation into the site of ischemic lesions in the rat brain. In addition to endogenous neural stem cells, also astrocytes and NG2-glia play an important role in neuro- and gliogenesis during ischemia/reperfusion. Their proliferation, migration as well as the proliferation and differentiation of GFP-labeled endogenous neural stem cells, will be investigated in response to the stereotactic delivery of morphogenes and growth factors into the lateral ventricles, using immunohistochemical and electrophysiological techniques. Our data will provide information about the role of morphogenes in brain regeneration after ischemia/ reperfusion in connection with the proliferation and differentiation of neural stem/progenitor cells as well as the proliferation and migration of astrocytes and NG2-glia. This study should also contribute to the development of new therapeutic approaches to the treatment of cerebral ischemia."@en . "1"^^ . "2015-03-02+01:00"^^ . "Induction of neuro- and gliogenesis in rat brain after ischemia - the role of morphogenes and growth factors in the nervous tissue regeneration."@en . . " astrocyty" . . . "0"^^ . " differenciace" . "2011-12-31+01:00"^^ . . . . "C\u00EDlem projektu je studovat regeneraci ischemicky po\u0161kozen\u00E9 nervov\u00E9 tk\u00E1n\u011B, se zam\u011B\u0159en\u00EDm na vliv morfogen\u016F (Sonic Hedgehog a Wnt-7a) a r\u016Fstov\u00FDch faktor\u016F na proliferaci, migraci a differenciaci endogenn\u00EDch neur\u00E1ln\u00EDch kmenov\u00FDch bun\u011Bk, a \u00FAlohu NG2-gli\u00ED a reaktivn\u00EDch astrocyt\u016F v neurogenezi a gliogenezi." . . . "\u0158e\u0161en\u00ED projektu prob\u011Bhlo v\u00FDte\u010Dn\u011B, jak z hlediska odborn\u00E9ho tak i z hlediska \u010Derp\u00E1n\u00ED finan\u010Dn\u00EDch prost\u0159edk\u016F."@cs . . "neuroregenerace" . "2011-04-16+02:00"^^ . . "GA305/09/0717" . . " NG2-glie" . " proliferace" . . "http://www.isvav.cz/projectDetail.do?rowId=GA305/09/0717"^^ . "The project was excellent, both in terms of expertise and in terms of disbursement of funds."@en .