. "V\u00FDsledky byly prezentov\u00E1ny na t\u0159ech konferenc\u00EDch a byly p\u0159ipraveny 2 publikace, z nich\u017E jedna do impaktovan\u00E9ho \u010Dasopisu. Vzhledem k tomu, \u017Ee z\u00EDskan\u00E9 v\u00FDsledky spl\u0148uj\u00ED vyty\u010Den\u00E9 c\u00EDle projektu, doporu\u010Dovali bychom, p\u0159esto \u017Ee u impaktovan\u00E9 publikace nebylo do"@cs . . "2003-04-01+02:00"^^ . "Micromanipulation techniques have an increasing importance within the range of methods of the contemporary cell biology and reproductive technologies. The aim of the project is a detailed study of the behaviour of cytoskeletal structures in cells in vitro subjected to of mechanical damage by micromanipulations. Various types of diploid and established human cell lines bearing different morphological markers will be used as model objects. The dynamic changes of cytoskeletal components (microtubules, microfilaments and intermediate filaments) will be followed as well as the influence of mechanical damage on mitotic divisions and intracellular transport. In addition, the role of the cytoskeleton in reparative processes after micromanipulation invasionwill be studied. Immunofluorescence detection of the cytoskeletal proteins using of the monoclonal antibodies, transmission and scanning electron microscopy will be the main experimental approaches. The results obtained will extend our understanding the"@en . . . . "Mikromanipula\u010Dn\u00ED techniky zauj\u00EDmaj\u00ED st\u00E1le d\u016Fle\u017Eit\u011Bj\u0161\u00ED m\u00EDsto v metod\u00E1ch sou\u010Dasn\u00E9 bun\u011B\u010Dn\u00E9 biologie a reproduk\u010Dn\u00EDch biotechnologi\u00ED. Tento projekt si klade za c\u00EDl detailn\u00ED studium reakc\u00ED jednotliv\u00FDch cytoskelet\u00E1ln\u00EDch komponent lidsk\u00FDch bun\u011Bk in vitro na mechanick\u00E9 po\u0161kozen\u00ED, vyvolan\u00E9 mikromanipula\u010Dn\u00EDmi z\u00E1sahy. Modelov\u00FDm objektem budou diploidn\u00ED i stabilizovan\u00E9 linie r\u016Fzn\u00FDch typ\u016F lidsk\u00FDch bun\u011Bk, kter\u00E9 se budou li\u0161it jednak morfologicky, jednak p\u0159\u00EDtomnost\u00ED odli\u0161n\u00FDch cytoskelet\u00E1ln\u00EDch a asociovan\u00FDch protein\u016F. Sledov\u00E1na bude dynamika zm\u011Bn z\u00E1kladn\u00EDch slo\u017Eek cytoskeletu (mikrotubuly, mikrofilamenta, intermedi\u00E1rn\u00ED filamenta), vlivy na mitotick\u00E9 d\u011Blen\u00ED, vnitrobun\u011B\u010Dn\u00FD transport a d\u00E1le obraz cytoskeletu a jeho role v repara\u010Dn\u00EDch procesech po mikromanipulac\u00EDch. Metodicky bude hlavn\u00ED t\u011B\u017Ei\u0161t\u011B spo\u010D\u00EDvat na imunofluorescen\u010Dn\u00ED detekci cytoskelet\u00E1ln\u00EDch protein\u016F pomoc\u00ED monoklon\u00E1ln\u00EDch protil\u00E1tek, d\u00E1le na vyu\u017Eit\u00ED transmisn\u00ED i skanovac\u00ED elektronov\u00E9 mikroskopie. Projekt by m\u011Bl p\u0159isp\u011Bt k detailn\u00EDmu pozn\u00E1n" . . "5"^^ . "Influence of micromanipulations on the cytoskeleton of human cells in vitro"@en . "5"^^ . . "0"^^ . . "1"^^ . "GA304/98/P222" . . . . . "Vliv mikromanipulac\u00ED na cytoskelet lidsk\u00FDch bun\u011Bk in vitro" . . "http://www.isvav.cz/projectDetail.do?rowId=GA304/98/P222"^^ . "0"^^ . . "Neuvedeno."@en . .