. "GA304/98/0604" . . "0"^^ . . . . . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA304/98/0604"^^ . . . "Anal\u00FDza histonov\u00E9ho modelu cystick\u00FDch dilatac\u00ED nefron\u016F z\u00E1rodku ku\u0159ete" . . . . "Odborn\u00FD p\u0159\u00EDnos projektu spo\u010D\u00EDv\u00E1 zejm\u00E9na v mechanismu cystick\u00E9 dilatace nefronu u z\u00E1rodku ku\u0159ete. Byla demonstrov\u00E1na vazba histonu na bu\u0148ky dut\u00E9ho syst\u00E9mu nefronu. Byl rovn\u011B\u017E zji\u0161\u0165ov\u00E1n vliv jin\u00FDch toxick\u00FDch agens (cis platina). D\u00E1le byly demonstrov\u00E1ny ad"@cs . "Mezi \u010Dast\u00E9 poruchy v\u00FDvoje nefron\u016F pat\u0159\u00ED cystick\u00E9 dilatace jejich tubul\u016F (CDT). Z mnoha hypot\u00E9z o p\u0159\u00ED\u010Din\u00E1ch jejich vzniku je zat\u00EDm \u017E\u00E1dn\u00E1 \u00FApln\u011B nevysv\u011Btluje. M\u00E1me k dispozici model CDT mesonefrick\u00FDch tubul\u016F ku\u0159ec\u00EDho z\u00E1rodku, kter\u00E9 lze standardn\u011B vyvol\u00E1vat intraamni\u00E1ln\u00ED aplikac\u00ED celkov\u00E9ho histonu z telec\u00EDho thymu v obdob\u00ED diferenciace nefron\u016F. Chceme prov\u00E9st kauz\u00E1ln\u00ED anal\u00FDzu tohoto modelu a zjistit sledov\u00E1n\u00EDm distribuce zna\u010Den\u00E9ho histonu ve tk\u00E1n\u00EDch z\u00E1rodku, na kter\u00E9 bu\u0148ky histon p\u016Fsob\u00ED, a kter\u00E9 dal\u0161\u00ED faktory ovliv\u0148uj\u00ED vyvol\u00E1n\u00ED CDT (d\u00E1vka, stadium v\u00FDvoje p\u0159i aplikaci, pohlav\u00ED z\u00E1rodk\u016F, inkuba\u010Dn\u00ED podm\u00EDnky a pod.). Metodami funk\u010Dn\u00ED morfologie chceme vy\u0161et\u0159it struktur\u00E1ln\u00ED a funk\u010Dn\u00ED vlastnosti indukovan\u00FDch CDT a jejich odchylky od normy. Z v\u00FDsledk\u016F t\u011Bchto \u0161et\u0159en\u00ED hodl\u00E1me odvodit teorii o mechanizmu vzniku CDT v na\u0161em modelu a konfrontovat ji s p\u0159edstavami o vzniku polycystick\u00E9 ledviny." . . . . . "10"^^ . "10"^^ . "Cystic dilatations of the tubules ( CDT ) are frequent disorders of nephron development. None of numerous hypotheses about the causes of their origin provides full explanation. We have a model of CDT of the chick mesonephric tubules constantly inducible by intraamniotic administration of the total calf thymus histone during the period of nephron differentiation. We plan to subject this model to causal analysis and to assess the target cells with the aid of histone labeling. We want to detect further factors influencing induction of CDT in this model and to examine differences in structural and functional properties of CDT and normal tubules with the aid of methods of functional morphology. From these results we aim to infere a theory on the mechanisms of CDT formation in our model and to confront it with the recent conceptions on polycystic kidney origin."@en . "1"^^ . "Analysis of the histone model of nephron cystic dilatations in the chick embryo"@en .