"The development and regeneration of muscle fibres starts from embryonic or adult myoblasts. The resulting phenotype is a combination of genetically fixed properties of myoblast cell lineages and of extrinsic, primarily neurogenic factors.Intrastructural fibre types of muscle spindles differ from each other and from extrafusal fibres by their ultrastructure,presence of both sensory and motor innervation and by the content of specific myosin heavy chain(MHC) isoforms. Differentiation of these distinctions depends on the morphogenetic influence of primary afferent neurones. It is, howerver, not known, whether the intrafusal phenotype can be induced in any myotube regardless of its cell line origin or only in a special predetermined intrafusal lineage(s) committed to differentiate into intrafusal muscle fibres. The aim of this project is to define the contribution of intrinsic myogenic properties of muscle cell lineage and extrinsic neurogenic factors exerted by sensory and motor innervation on the diffe"@en . . . . "Myogeneze i regenerace kostern\u00EDch sval\u016F vych\u00E1z\u00ED z embryon\u00E1ln\u00EDch respektive dosp\u011Bl\u00FDch myoblast\u016F a v\u00FDsledn\u00FD fenotyp svalov\u00FDch vl\u00E1ken je d\u00E1n kombinac\u00ED genetick\u00FDch vlastnost\u00ED bun\u011B\u010Dn\u00E9 linie myoblast\u016F a vlivu vn\u011Bj\u0161\u00EDch, p\u0159edev\u0161\u00EDm neurogenn\u00EDch faktor\u016F. T\u0159i typy intrafuz\u00E1ln\u00EDch vl\u00E1ken nervosvalov\u00FDch v\u0159et\u00E9nek se odli\u0161uj\u00ED od sebe navz\u00E1jem a od extrafuz\u00E1ln\u00EDch vl\u00E1ken jak morfologick\u00FDmi charakteristikami a p\u0159\u00EDtomnost\u00ED senzorick\u00E9 i motorick\u00E9 inervace, tak i obsahem specifick\u00FDch t\u011B\u017Ek\u00FDch \u0159et\u011Bzc\u016F myozinu (T\u0158M). Jejich v\u00FDvoj je indukov\u00E1n morfogenetick\u00FDm p\u016Fsoben\u00EDm senzorick\u00FDch termin\u00E1l. Nen\u00ED v\u0161ak zn\u00E1mo, zda se intrafuz\u00E1ln\u00ED vl\u00E1kna diferencuj\u00ED ze speci\u00E1ln\u00ED predeterminovan\u00E9 bun\u011B\u010Dn\u00E9 linie myoblast\u016F nebo ze stejn\u00FDch bun\u011B\u010Dn\u00FDch lini\u00ED, jako extrafuz\u00E1ln\u00ED svalov\u00E1 vl\u00E1kna. C\u00EDlem projektu je ur\u010Dit pomoc\u00ED ultrastruktur\u00E1ln\u00ED anal\u00FDzy a imunocytochemick\u00E9 determinace izoformem T\u0158M za r\u016Fzn\u00FDch experiment\u00E1ln\u00EDch podm\u00EDnek pod\u00EDl geneticky fixovan\u00FDch vlastnost\u00ED bun\u011B\u010Dn\u00E9 linie a pod\u00EDl vn\u011Bj\u0161\u00EDch faktor\u016F, zejm\u00E9na senzorick\u00E9 a motorick\u00E9 inervace, na dif" . . . "Tato experiment\u00E1ln\u00ED pr\u00E1ce byla zam\u011B\u0159ena na studium vlivu inervace a vlivu hormon\u016F \u0161t\u00EDtn\u00E9 \u017El\u00E1zy na v\u00FDvoj fenotyp\u016F specifick\u00FDch pro intrafuz\u00E1ln\u00ED vl\u00E1kna svalov\u00FDch v\u0159et\u00E9nek. V\u00FDsledky pokus\u016F byly hodnoceny mikroskopicky v \u0159ezech zpracovan\u00FDch pro ultrastruktur"@cs . . "Anal\u00FDza vlivu myogenn\u00EDch a neurogenn\u00EDch faktor\u016F na expresi specifick\u00E9ho fenotypu intrafuz\u00E1ln\u00EDch svalov\u00FDch vl\u00E1ken" . "1"^^ . . "Analysis of the role of myogenic and neurogenic factors regulating the expression of specific phenotypes in intrafusal muscle fibres."@en . "0"^^ . "GA304/97/0516" . . . "24"^^ . . . "0"^^ . "24"^^ . . . . "http://www.isvav.cz/projectDetail.do?rowId=GA304/97/0516"^^ .