. . . . . . "Valn\u00E1 \u010D\u00E1st tvorby ribozomu prob\u00EDh\u00E1 v jad\u00E9rku. V posledn\u00EDch n\u011Bkolika letech do\u0161lo pou\u017Eit\u00EDm biochemick\u00FDch a molekul\u00E1rn\u011B biologick\u00FDch p\u0159\u00EDstup\u016F k z\u00E1sadn\u00EDmu prohlouben\u00ED na\u0161ich znalost\u00ED o biogenezi ribozom\u016F. Nicm\u00E9n\u011B pou\u017Eit\u00E9 in vitro p\u0159\u00EDstupy nemohou obs\u00E1hnout slo\u017Eitou molekul\u00E1rn\u00ED architekturu jad\u00E9rka, zahrnuj\u00EDc\u00ED v sob\u011B tak\u00E9 prostorov\u00E9 uspo\u0159\u00E1d\u00E1n\u00ED ribosom\u00E1ln\u00EDch gen\u016F. K prohlouben\u00ED na\u0161ich znalost\u00ED v tomto ohledu proto chceme vyu\u017E\u00EDt na\u0161i st\u00E1vaj\u00EDc\u00ED expert\u00EDzu ve sv\u011Bteln\u00E9 a elektronov\u00E9 mikroskopii, imunocytochemii ain situ hybridizaci ke studiu funk\u010Dn\u00ED architektury jad\u00E9rka v synchronizovan\u00FDch kultur\u00E1ch sav\u010D\u00EDch i lidsk\u00FDch bun\u011Bk, v\u010Detn\u011B bun\u011Bk transfektovan\u00FDch GFP konstrukty. Speci\u00E1ln\u011B se v tomto projektu chceme soust\u0159edit na \u010Dasov\u00E9 a prostorov\u00E9 uspo\u0159\u00E1d\u00E1n\u00ED transkrip\u010Dn\u011B aktivn\u00EDch a neaktivn\u00EDch ribozom\u00E1ln\u00EDch gen\u016F a souhru jejich transkrip\u010Dn\u00EDch a replika\u010Dn\u00EDch aktivit, a rovn\u011B\u017E na regula\u010Dn\u00ED proteinov\u00E9 a ribonukleoproteinov\u00E9 komplexy kontroluj\u00EDc\u00ED transkripci a replikaci ve vztahu ke struktu\u0159e jad\u00E9rka." . "0"^^ . "Funkce, struktura a dynamika jadern\u00E9 tov\u00E1rny na ribosomy" . . "1"^^ . "Function, structure and dynamics of the nuclear ribosome-producing machinery"@en . . "http://www.isvav.cz/projectDetail.do?rowId=GA304/04/0692"^^ . . . . . "0"^^ . . . . . "Most of the ribosome biogenesis takes place in nucleoli and an exciting progress of the molecular knowledge about the ribosome biogenesis was achieved during the past few years through biochemical and molecular biology approches. However, these in vitro systems cannot take into account the complex molecular architecture of the nucleous and the spatial organization of the ribosomal gene tandem repeated clusters. In order to expand our information in this respect, we wish, using our expertise in light andelectron microscopy, immunocytochemistry and in situ hybridization, to analyze the functional architecture of the nucleous in synchronized mammalian and human cells, including GFP-transfected cells. Specially in this project, we would like to focus on the temporal and spatial arrangement of transcriptionally active and inactive ribosomal genes, the interplay between their replication and transcription activities, the nucleolar transcription and replication machinery as well as the regulatory protein"@en . "The project deals with the functional organization of nucleolus, the ribosome factory. Function of nucleolus is regulated at several levels of the structural organization. The first level is represented by positioning of the chromosomes carrying the clus"@en . . "Neuvedeno."@en . "15"^^ . . "GA304/04/0692" . . "15"^^ . "2007-10-16+02:00"^^ . . "Projekt se zab\u00FDval funk\u010Dn\u00ED organizac\u00ED jad\u00E9rka, tov\u00E1rny na ribozomy. Funkce jad\u00E9rka je ovlivn\u011Bna n\u011Bkolika stupni architektur\u00E1ln\u00EDho uspo\u0159\u00E1d\u00E1n\u00ED. Prvn\u00EDm regula\u010Dn\u00EDm stupn\u011Bm je uspo\u0159\u00E1d\u00E1n\u00ED chromozom\u016F nesouc\u00EDch opakuj\u00EDc\u00ED se ribozom\u00E1ln\u00ED geny (tzv. nukleol\u00E1rn\u00ED org"@cs .