"GA304/03/1189" . . "2009-01-15+01:00"^^ . "The project describes application of superparamagnetic nanoparticles based on iron oxides as MR contrast agents for cell labeling. Transplanted labeled cells and pancreatic islets have been detected in vivo in the brain, spinal cord and liver by using th"@en . . . "22"^^ . "0"^^ . . "22"^^ . . . "Pou\u017Eit\u00ED superparamagnetick\u00FDch nano\u010D\u00E1stic pro MR zobrazov\u00E1n\u00ED implantovan\u00FDch bun\u011Bk" . "1"^^ . "\u0158e\u0161en\u00ED projektu uk\u00E1zalo na \u0159adu mo\u017Enost\u00ED vyu\u017E\u00EDv\u00E1n\u00ED superparamagnetick\u00FDch nano\u010D\u00E1stic na b\u00E1zi oxid\u016F \u017Eeleza jako MR kontrastn\u00EDch l\u00E1tek pro zna\u010Den\u00ED kmenov\u00FDch bun\u011Bk. Touto technikou byla pops\u00E1na poloha t\u011Bchto transplantovan\u00FDch bun\u011Bk v\u00A0mozku, m\u00ED\u0161e a inzul\u00EDnov\u00FD"@cs . . . "http://www.isvav.cz/projectDetail.do?rowId=GA304/03/1189"^^ . "1"^^ . . . . . "Centr\u00E1ln\u00ED nervov\u00FD syst\u00E9m (CNS) m\u00E1 velmi omezenou schopnost regenerace. Dosp\u011Bl\u00E9 neurony nejsou schopny se d\u00E1le d\u011Blit a neur\u00E1ln\u00ED kmenov\u00E9 bu\u0148ky, a\u010Dkoliv byla prok\u00E1z\u00E1na jejich existence i v dosp\u011Bl\u00E9m mozku, se mohou zapojit do regenera\u010Dn\u00EDch proces\u016F jen velmiomezen\u011B. Jedn\u00EDm z mo\u017En\u00FDch zp\u016Fsob\u016F, jak regeneraci CNS vyvolat, je kultivace neur\u00E1ln\u00EDch kmenov\u00FDch bun\u011Bk in vitro a jejich implantace do CNS posti\u017Een\u00E9ho degenerativn\u00EDm onemocn\u011Bn\u00EDm, ischemi\u00ED nebo traumatem. Transplantovan\u00E9 bu\u0148ky jsou obvykle ozna\u010Denyfluorescen\u010Dn\u00EDm barvivem a jejich migrace a diferenciace je na zv\u00ED\u0159ec\u00EDch modelech sledov\u00E1na prost\u0159ednictv\u00EDm imunohistochemick\u00FDch technik. P\u0159edkl\u00E1dan\u00FD projekt nab\u00EDz\u00ED mo\u017Enost implantovat embryon\u00E1ln\u00ED kmenov\u00E9 bu\u0148ky nebo kmenov\u00E9 bu\u0148ky kostn\u00ED d\u0159en\u011B ozna\u010Den\u00E9superparamagnetick\u00FDmi nanopartikulemi zv\u00ED\u0159at\u016Fm s l\u00E9zemi CNS (nap\u0159\u00EDklad ischemick\u00E9 a fotochemick\u00E9 l\u00E9ze kortexu, transsekce m\u00EDchy). Osud implantovan\u00FDch bun\u011Bk a jejich chov\u00E1n\u00ED v \u010Dase bude n\u00E1sledn\u011B mo\u017En\u00E9 dynamicky sledovat in vivo vyu\u017Eit\u00EDm technik magnetick\u00E9" . "The use of superparamagnetic nanoparticles for MR imaging of implanted cells"@en . . . . . . . "The central nervous system, unlike many other tissues, has a limited capacity for self-repair; mature nerve cells lack the ability to regenerate, and neural stem cells, although they exist even in the adult brain, have a limited ability to generate newfunctional neurons in response to injury. For this reason there have been several effective methods developed for culturing different types of stem cells and transplanting them into animal models of degenerative disorders, ischemia or injury. Thetransplanted cells are usually labeled with fluorencent %22cell trackers%22, and the migration and differentiation of the transplanted cells are observed and evaluated on fixed tissue using immunohistochemical methods. The aim of this study is to transplantembryonic or bone marrow stem cells labeled with superparamagnetic nanoparticles into different animal models of brain and spinal cord injury (i.e., cortical photochemical and ischemic lesion, spinal cord photochemical lesion and transsection) and"@en . . "Neuvedeno."@en . . .