"1"^^ . "2006-01-01+01:00"^^ . "1"^^ . "Charakterizace protein\u016F sekretovan\u00FDch sam\u010D\u00EDm pohlavn\u00EDm traktem a jejich \u00FAloha v jednotliv\u00FDch kroc\u00EDch reproduk\u010Dn\u00EDho procesu" . . . . . "0"^^ . . . " sperm surface proteins" . . . "9"^^ . . "9"^^ . "2009-10-22+02:00"^^ . "GA303/06/0895" . . "Projekt byl zam\u011B\u0159en na proteiny produkovan\u00E9 sam\u010D\u00EDm reproduk\u010Dn\u00EDm traktem a studium jejich vazebn\u00FDch vlastnost\u00ED pro lep\u0161\u00ED pochopen\u00ED jejich mo\u017En\u00E9 \u00FAlohy v reproduk\u010Dn\u00EDm procesu. Na\u0161e v\u00FDsledky se t\u00FDkaj\u00ED p\u0159edev\u0161\u00EDm separace a charakterizace protein\u016F kan\u010D\u00ED semenn"@cs . "2008-12-31+01:00"^^ . "The project was focused on proteins secreted by the male reproductive tract and their binding properties for better understanding their role in the reproductive process. Our results deal with separation and characterization of boar seminal plasma protein"@en . . . "Characterization of proteins secreted by the male reproductive tract and their role in individual steps of the reproduction process"@en . "male reproductive tract; sperm surface proteins; fertilization; sperm oviductal reservoir"@en . "http://www.isvav.cz/projectDetail.do?rowId=GA303/06/0895"^^ . . "The project is aimed to study the role of sperm surface proteins in selected phases of the reproduction process. These events are connected with remodelling of protein coating layers, which are formed by proteins on the sperm surface. There exists only limited information about the function of sperm surface proteins during sperm epididymal maturation or modification of the sperm surface in the female reproductive tract that is related to the formation of the sperm oviductal reservoir, sperm capacitation, and primary contact of gametes. Attention will be focused on (i) characterization and identification of proteins of boar epididymal fluid and on the study of the formation and modification of the protein layer coating the epididymal sperm during passage through the epididymis; (ii) the study of the origin of sperm surface proteins secreted by the male reproductive tract (isolation of their mRNA); (iii) the study of the participation of sperm surface proteins in the formation of the sperm"@en . . . . "2008-04-25+02:00"^^ . . . " fertilization" . "male reproductive tract" . "C\u00EDlem p\u0159edkl\u00E1dan\u00E9ho projektu je \u00FAloha povrchov\u00FDch protein\u016F spermie ve vybran\u00FDch f\u00E1z\u00EDch reproduk\u010Dn\u00EDho procesu. Tyto ud\u00E1losti jsou spojeny s\u00A0p\u0159em\u011Bnou proteinov\u00E9 vrstvy, tvo\u0159enou proteiny adsorbovan\u00FDmi na povrchu spermie. Existuj\u00ED pouze omezen\u00E9 informace o funkci t\u011Bchto povrchov\u00FDch protein\u016F b\u011Bhem zr\u00E1n\u00ED spermi\u00ED v\u00A0epididymu nebo o p\u0159em\u011Bn\u00E1ch povrchu spermi\u00ED v\u00A0sami\u010D\u00EDm pohlavn\u00EDm traktu, kter\u00E9 souvis\u00ED s\u00A0vytvo\u0159en\u00EDm ovidukt\u00E1ln\u00EDho reservo\u00E1ru spermi\u00ED, kapacitac\u00ED spermi\u00ED a prim\u00E1rn\u00ED vazbou spermie na vaj\u00ED\u010Dko. Pozornost bude zam\u011B\u0159ena na (i) charakterizaci a identifikaci protein\u016F kan\u010D\u00ED epididym\u00E1ln\u00ED tekutiny a na studium tvorby a p\u0159em\u011Bny proteinov\u00E9 vrstvy pokr\u00FDvaj\u00EDc\u00ED epididym\u00E1ln\u00ED spermie b\u011Bhem jejich pr\u016Fchodu epididymem; (ii) na ur\u010Den\u00ED p\u016Fvodu povrchov\u00FDch protein\u016F spermie sekretovan\u00FDch sam\u010D\u00EDm reproduk\u010Dn\u00EDm traktem (izolace jejich mRNA); (iii) na studium \u00FA\u010Dasti povrchov\u00FDch protein\u016F spermie na vytvo\u0159en\u00ED ovidukt\u00E1ln\u00EDho rezervo\u00E1ru spermi\u00ED, p\u0159i\u00A0uvoln\u011Bn\u00ED spermi\u00ED z\u00A0oviduktu, v\u00A0pr\u016Fb\u011Bhu kapacitace spermi\u00ED a" .