"Experimental model of colitis. Dysregulation of the immune response to the gut microflora"@en . . . "1"^^ . . . "Neuvedeno."@en . "1"^^ . "Grantov\u00FD projekt \u0159e\u0161il nesm\u00EDrn\u011B klinicky d\u016Fle\u017Eitou a m\u00E1lo prob\u00E1danou oblast nespecifick\u00FDch st\u0159evn\u00EDch z\u00E1n\u011Bt\u016F. Je sou\u010D\u00E1st\u00ED dlouhodob\u00E9 v\u00FDzkumn\u00E9 pr\u00E1ce zku\u0161en\u00E9ho a sv\u011Btov\u011B zn\u00E1m\u00E9ho kolektivu v\u011Bdc\u016F. Spoluautorem byl v\u00FDzna\u010Dn\u00FD klinick\u00FD pracovn\u00EDk - gastroenterolog"@cs . . . "GA303/00/1370" . . "The aim of the project is to determine a role CD4+ CD45RBhigh T lymphocytes subset and its interaction with intestinal microflora play in the induction of experimental colitis occuring in CD4+ CD45RBhigh - reconstituted conventional SCID mice. Effector CD4+ CD45RBhigh T lymphocytes isolated from immunocompetent syngeneic donors by high speed flow cytometric sorting will be transfered into SCID recipients kept under either conventional (CV) or germ-free (GF) conditions. Morphological and phenotypic changes in inflamed colon will be studied by histological techniques and monoclonal antibodies directed against lineage-restricted surface markers. A role of individual compounds of gut microflora in the disease pathogenesis wil be stidied in ex-germ free, CD4+ CD45RBhigh T cell reconstituted SCID mice monoassociated and oligoassociated with individual bacterial strains and their mixtures, respectively. This experimental approach will amke it possible to determine how the pathogenic action of effecto"@en . . "Experiment\u00E1ln\u00ED modely nespecifick\u00FDch st\u0159evn\u00EDch z\u00E1n\u011Bt\u016F. Dysregulace imunitn\u00ED odpov\u011Bdi ve vztahu ke st\u0159evn\u00ED mikrofl\u00F3\u0159e" . . . . . . . . . "2008-05-19+02:00"^^ . . "18"^^ . . "18"^^ . "0"^^ . "http://www.isvav.cz/projectDetail.do?rowId=GA303/00/1370"^^ . "C\u00EDlem projektu je ur\u010Dit jakou \u00FAlohu hraje subpopulace CD4+ T lymfocyt\u016F (CD45RB high) a jej\u00ED aktivace antigeny norm\u00E1ln\u00ED st\u0159evn\u00ED fl\u00F3ry i indukci experiment\u00E1ln\u00ED kolitidy. Provedeme proto p\u0159enos subpopulac\u00ED lymfocyt\u016F CD4+CD45RB high do imunodeficitn\u00ED konven\u010Dn\u00EDch a bezmikrobn\u00EDch SCID my\u0161\u00ED (s vrozen\u00FDm t\u011B\u017Ek\u00FDm kombinovan\u00FDm defektem pro T i B lymfocyty). Bu\u0148ky pro p\u0159enos budou isolov\u00E1ny ze slezin a z lymfatick\u00E9 st\u0159evn\u00ED tk\u00E1n\u011B pln\u011B imunokompetentn\u00EDch BALB/c my\u0161\u00ED. Zv\u00ED\u0159ata budou chov\u00E1na v bezmikrobn\u00EDch a konven\u010Dn\u00EDch podm\u00EDnk\u00E1ch. P\u0159i v\u00FDvoji chronick\u00E9 kolitidy budou sledov\u00E1ny zm\u011Bny st\u0159evn\u00ED sliznice morfologicky a metodou imunohistochemie (v\u010Detn\u011B produkce cytokin\u016F). Pomoc\u00ED monoklon\u00E1ln\u00EDch protil\u00E1tek FACSovou analysou bude stanoveno zastoupen\u00ED jednotliv\u00FDch bun\u011B\u010Dn\u00FDch subpopulac\u00ED v z\u00E1n\u011Btliv\u00E9 tk\u00E1ni tlust\u00E9ho st\u0159eva. St\u0159evn\u00ED trakt bezmikrobn\u00EDch d\u00E1rc\u016F i p\u0159\u00EDjemc\u016F bude os\u00EDdlen definovan\u00FDmi kmeny bakteri\u00ED a to n\u00E1m umo\u017En\u00ED ur\u010Dit bakteri\u00E1ln\u00ED komponent zodpov\u011Bdn\u00E9 za v\u00FDvoj kolitidy. Tento experiment\u00E1ln\u00ED model n\u00E1m umo\u017En\u00ED" .