. . "C\u00EDle projektu\u00A0formulovan\u00E9 v p\u016Fvodn\u00EDm n\u00E1vrhu s modifikacemi zd\u016Fvodn\u011Bn\u00FDmi v d\u00EDl\u010D\u00EDch zpr\u00E1v\u00E1ch byly spln\u011Bny. Jako prvn\u00ED na sv\u011Bt\u011B jsme provedli komparativn\u00ED proteomickou anal\u00FDzu leukemick\u00FDch bun\u011Bk BM2 a jejich deriv\u00E1t\u016F v pr\u016Fb\u011Bhu termin\u00E1ln\u00ED diferenciace vyvola"@cs . "http://www.isvav.cz/projectDetail.do?rowId=GA301/03/1055"^^ . "We\u00A0reached the goals described in the\u00A0project\u00A0proposal\u00A0with modifications specified in the reports\u00A0for years 2003 and 2004.\u00A0We performed the first comparative proteomics analysis of leukemic monoblasts BM2 undergoing terminal differentiation upon treatme"@en . . "1"^^ . . . . "Neuvedeno."@en . . "1"^^ . "Onkogen v-myb viru pta\u010D\u00ED myeloblast\u00F3zy zp\u016Fsobuje akutn\u00ED myeloblastickou leuk\u00E9mii in vivo a transformuje myelomonocytick\u00E9 bu\u0148ky in vitro. Tento onkogen k\u00F3duje transkrip\u010Dn\u00ED faktor v-Myb, kter\u00FD je schopen zm\u011Bnou expresn\u00EDch profil\u016F sv\u00FDch c\u00EDlov\u00FDch gen\u016F a/nebointerakcemi s jin\u00FDmi proteiny vyvolat transformaci bu\u0148ky. Linie monoblast\u016F transformovan\u00FDch onkogenem v-myb (BM2) p\u0159edstavuje vhodn\u00FD model pro studium mechanismu \u00FA\u010Dinku v-Myb, proto\u017Ee p\u016Fsoben\u00EDm ur\u010Dit\u00FDch \u010Dinidel,jako nap\u0159. forbolov\u00FDch ester\u016F (TPA),trichostatinu A (TSA) a kyseliny retinov\u00E9 (RA) lze p\u0159ekonat zhoubn\u00FD \u00FA\u010Dinek transformuj\u00EDc\u00EDho onkoproteinu a vyvolat termin\u00E1ln\u00ED diferenciaci, zastaven\u00ED r\u016Fstu a/nebo apopt\u00F3zu bun\u011Bk. Krom\u011B toho m\u016F\u017Ee b\u00FDt citlivost bun\u011Bk BM2 k uveden\u00FDm induktor\u016Fm v\u00FDznamn\u011Bovlivn\u011Bna ektopickou expres\u00ED ur\u010Dit\u00FDch gen\u016F (RAR ?, RXR ?, c-myb, v-jun, c-jun, v-fos, c-fos, CBP, p300, p53, bcl-2). Tyto \u00FA\u010Dinky pravd\u011Bpodobn\u011B vypl\u00FDvaj\u00ED ze specifick\u00FDch interakc\u00ED proteinu v-Myb a/nebo produkt\u016F jeho c\u00EDlov\u00FDch gen\u016F s elementy bun\u011B\u010Dn\u00FDch" . "2009-01-15+01:00"^^ . . "The v-myb oncogene of avian myeloblastosis virus causes acute myeloblastic leukemia in vivo and transforms myelomonocytic cells in vitro. It codes for a v-Myb transcription factor that changes expression pattern of its target genes and/or interacts withother proteins thus causing transformation of the cell. The model line of v-Myb-transformed monoblasts BM2 can undergo terminal differentiation, growth arrest and/or apoptosis in response to extracellular stimuli such as phorbol esters (TPA),trichostatin A (TSA) and retinoic acid (RA). Sensitivity of BM2 cells to these agents can be significantly affected by ectopic expression of certain genes (RAR ?, RXR ?, c-myb, v-jun, c-jun, v-fos, c-fos, CBP, p300, p53, bcl-2). These effects result fromspecific interactions of v-Myb protein and/or its target gene products with cellular signaling pathways directed by individual inducers. This project is designed to investigate the network of intracellular pathways that possess decisive role in"@en . "Studium molekul\u00E1rn\u00EDch mechanism\u016F zp\u016Fsobuj\u00EDc\u00EDch supresi v-Myb s vyu\u017Eit\u00EDm p\u0159\u00EDstup\u016F proteomiky" . . . . . . "GA301/03/1055" . . . . . . "Study of molecular mechanisms causing v-Myb suppression using proteomics-based approach"@en . . "23"^^ . "0"^^ . . "23"^^ .