. . . . "1"^^ . "Neuvedeno."@en . . "The project was designed to explore the expression of STAT 1, STAT 3 and SOCS 3 proteins in normal and cancer cells, and further to answer the question whether IFN-induced phosphorylation (activation) of the above mentioned STAT peptides at tyrosine (Tyr"@en . . . . "Proteiny rodiny STAT jsou transduk\u010Dn\u00ED a transkrip\u010Dn\u00ED faktory, kter\u00E9 jsou aktivov\u00E1ny vazbou cytokin\u016F na bun\u011B\u010Dn\u00E9 receptory a n\u00E1sledn\u011B zprost\u0159edkov\u00E1vaj\u00ED p\u0159enos sign\u00E1l\u016F do bun\u011B\u010Dn\u00E9ho j\u00E1dra. V\u00FDznamn\u011B se pod\u00EDlej\u00ED na procesech bun\u011B\u010Dn\u00E9ho r\u016Fstu, diferenciace aapopt\u00F3zy. Zat\u00EDmco existuje \u0159ada \u00FAdaj\u016F o mechanismech STAT-dependentn\u00ED sign\u00E1ln\u00ED transdukce, defekty t\u011Bchto proces\u016F v n\u00E1dorov\u00E9 bu\u0148ce nejsou doposud objasn\u011Bny. C\u00EDlem projektu je analyzovat hladiny protein\u016F STAT 1 a STAT 3 v norm\u00E1ln\u00EDch i malign\u00EDch bu\u0148k\u00E1ch, aodpov\u011Bd\u011Bt na ot\u00E1zku zda interferony a TNF-? indukov\u00E1na fosforylace t\u011Bchto peptid\u016F na Tyr 701 a Ser 727, a rovn\u011B\u017E transdukce sign\u00E1l\u016F do j\u00E1dra, jsou v n\u00E1dorov\u00E9 bu\u0148ce patologicky zm\u011Bn\u011Bny. Dal\u0161\u00EDm c\u00EDlem projektu je studovat \u00FAlohu STAT 1/STAT 3 aktivace vtranskripci genu p21WAF1/CIP1, p53, bcl-2, a bax v souvislosti s abnormalitami bun\u011B\u010Dn\u00E9ho r\u016Fstu a apopt\u00F3zy malign\u00ED bu\u0148ky a jej\u00ED rezistenc\u00ED na biologick\u00E9 \u00FA\u010Dinky interferon\u016F. V\u00FDsledky p\u0159isp\u011Bj\u00ED k bli\u017E\u0161\u00EDmu pochopen\u00ED molekul\u00E1rn\u00EDch mechanism\u016F z\u00E1visl\u00FDch na STAT" . "Studium mechanism\u016F transduk\u010Dn\u00ED a transkrip\u010Dn\u00ED aktivity STAT 1a STAT 3 protein\u016F v p\u0159enosu cytokinov\u00FDch sign\u00E1l\u016F v norm\u00E1ln\u00ED a n\u00E1dorov\u00E9 bu\u0148ce" . . . "0"^^ . . . . . . "Projekt byl zam\u011B\u0159en na studium rozd\u00EDl\u016F v expresi transduk\u010Dn\u00EDch a transkrip\u010Dn\u00EDch protein\u016F STAT 1 a STAT 3, v\u010Detn\u011B jejich endogenn\u00EDho inhibitoru SOCS 3, mezi norm\u00E1ln\u00ED a n\u00E1dorovou bu\u0148kou a na definov\u00E1n\u00ED p\u0159edpokl\u00E1dan\u00E9 patologick\u00E9 aktivace (fosforylace) STAT"@cs . . . "GA301/03/0370" . "Analysis of STAT 1 and STAT 3 mediated cytokine signaling in normal and cancer cells"@en . "STATs are multi protein transducers and transcriptional factors that are activated by attachment of cytokines to the cell surface, and mediate transport of signals into the nucleus. The molecular nature of these events and their abnormalities in cancercells have not yet been fully elucidated. The aim of the project is to analyze the STAT 1 and STAT 3 levels in normal as well as tumor cells, and to establish whether the capability of both peptides to undergo IFNs- and TNF- ? -induced phosphorylation atTyr 701 and Ser 727 residues, and to transduce signals into the nucleus, is pathologically changed in cancer. The involvement of normal and defected phosphorylation of STAT 1/STAT 3 in the transcription of p21WAF1/CIP1, p53, bcl-2, and bax genes will beexamined on the protein level. The results will be correlated with the growth patterns, apoptotic pathways and growth inhibitory effects of IFNs. The project is expected to highlight cytokine signaling cascades mediated by STAT 1/STAT 3 proteins and"@en . "http://www.isvav.cz/projectDetail.do?rowId=GA301/03/0370"^^ . "2009-01-15+01:00"^^ . . "8"^^ . "8"^^ . . "0"^^ .